基于全基因组关联分析揭示肉鸡腿病发生的遗传机制

doi:10.11843/j.issn.0366-6964.2024.01.011

Abstract

Abstract: This study aimed to reveal the genetic mechanism of leg disease in broilers, with the objective of mitigating the incidence of leg disease in broilers. This study employed a comprehensive approach involving 2 331 white-feathered broilers of multi-strain and multi-generation of "Guangming No. 2" independently bred in China. The assessment of leg health and diseases was performed by X-ray analysis at 42 days of age. For genotyping, the "Jingxin No.1" 55K SNP microarray was used for all individuals, and the genome-wide association study (GWAS) of leg health phenotypes was performed utilizing a logistic regression model tailored for dichotomous traits via plink software. After the integration and meticulous quality control of microarray data across diverse populations, a total of 2 330 individuals (including 2 256 healthy and 74 leg disease subjects) and 30 414 SNPs loci were deemed suitable for subsequent analysis. Through the GWAS analysis, 5 potential SNPs loci, distributed on chromosomes 6 and 18, were identified. Additionally, the investigation led to the annotation of 3 functional candidate genes, namely TBCD, SIRT1, and PBLD, which are directly related to leg disease. This study results provide important genetic loci and candidate genes for the healthy leg phenotype of white-feathered broilers, and provides a genetic basis for advancing the breeding process of broiler disease resistance.

Key words: white-feathered broiler, leg disease, genome-wide association analysis, candidate genes

CLC Number:

S831.2

TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.

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Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis

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