泌乳早期奶牛瘤胃微生物与牛奶脂肪酸组成的变化

doi:10.11843/j.issn.0366-6964.2022.09.018

Abstract

Abstract: This experiment was conducted to study the changes of rumen microbe and milk fatty acid composition during early lactation. In this study, 50 Holstein cows with similar parity, lactation days and milk yield were selected from a dairy farm in Jiangsu province. Milk samples and rumen fluid samples from 7 days of lactation (d7) and 30 days of lactation (d30) were collected. Feed intake, milk yield, milk composition and milk fatty acid content were detected. Meanwhile, the composition of rumen microbes was analyzed by 16S rRNA sequencing technology. The results showed that dry matter intake (15.79 kg·d^-1) and milk yield (26.81 kg) of d7 were significantly lower than those of d30 (dry matter intake:18.87 kg·d^-1; milk yield:37.47 kg)(P<0.01). Milk fat percentage, milk protein percentage and somatic cell score of d7 were extremely significantly higher than those of d30 (P<0.01). The contents of C6:0, C8:0, C10:0, C12:0, C14:0, C14:1trans9, C14:1cis9, C15:1trans10, medium chain fatty acids (MCFA) and saturated fatty acids (SFA) of d7 were significantly lower than those of d30 (P<0.05), on the contrary, the proportion of C17:0, C17:1cis10, C18:1trans6, C18:1trans11, C18:1cis9, C18:1cis11, C19:1trans10, C22:5cis4,7,10,13,16, long chain fatty acids (LCFA), monounsaturated fatty acids (MUFA) and trans fatty acids (TRANS) were significantly higher than those of d30 (P<0.05). 16S rRNA sequencing results showed that rumen bacterial richness and diversity of dairy cows during early lactation were not significantly changed (P>0.05). At the genus level, the abundance of Ruminiclostridium_1, Ruminococcaceae_UCG_014, Ruminococcaceae_UCG_005, Clostridium_sensu_stricto_1 and Dialister in rumen of d7 were significantly lower than those of d30 (P<0.05), while the abundance of Sphaerochaeta, Campylobacter and Eubacterium_saphenum_group were significantly higher than those of d30 (P<0.05). In addition, the association analysis between milk fatty acids and rumen microbiome data showed that rumen microbes had little influence on milk MCFA content, while cellulose-degrading bacteria (Ruminiclostridium_1, Ruminococcaceae_UCG_005) were significantly negatively correlated with the contents of C17:0, C18:1trans6, C18:1trans11 and C19:1trans10 in milk. In conclusion, changes in physiology and feed intake of dairy cows in early lactation lead to changes in rumen microbial composition, which lead to changes in microbial metabolites and metabolic pathways, and ultimately lead to changes in milk production and milk composition. The current study provides a scientific basis for analyzing the mechanism of rumen microorganisms regulating milk fat metabolism, and also provides a theoretical basis for regulating postpartum dairy cow nutrition and improving the quality of raw milk.

Key words: dairy cow, early lactation, rumen microorganisms, milk fat metabolism, correlation analysis

CLC Number:

S823

HU Liping, SHEN Ziliang, WANG Quan, YU Zitong, ZHANG Qiqi, MAO Yongjiang, YANG Zhangping, ZHANG Huimin. Changes of Rumen Microbe and Milk Fatty Acid Composition during Early Lactation[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3018-3028.

References 40

[1]	蒋涛.瘤胃微生物重塑对围产后期奶牛采食量和采食行为的影响[D].北京:中国农业大学, 2018.JIANG T.Influence of rumen microbial reshaping on feed intake and feeding behavior of Holstein Cows during the late perinatal period[D].Beijing:China Agricultural University, 2018.(in Chinese)
[2]	樊永亮.奶牛泌乳早期乳脂肪酸变化及乳腺组织转录调控分析[D].扬州:扬州大学, 2017.FAN Y L.Fatty acids changes of milk and transcriptional regulation analysis of mammary gland tissue in the early milk of dairy cows[D].Yangzhou:Yangzhou University, 2017.(in Chinese)
[3]	JOUANY J P.Optimizing rumen functions in the close-up transition period and early lactation to drive dry matter intake and energy balance in cows[J].Anim Reprod Sci, 2006, 96(3-4):250-264.
[4]	ELOLIMY A A, ARROYO J M, BATISTEL F, et al.Association of residual feed intake with abundance of ruminal bacteria and biopolymer hydrolyzing enzyme activities during the peripartal period and early lactation in Holstein dairy cows[J].J Anim Sci Biotechnol, 2018, 9:43.
[5]	XU W, VERVOORT J, SACCENTI E, et al.Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation[J].J Dairy Sci, 2020, 103(5):4795-4805.
[6]	POULSEN N A, HEIN L, KARGO M, et al.Realization of breeding values for milk fatty acids in relation to seasonal variation in organic milk[J].J Dairy Sci, 2020, 103(3):2434-2441.
[7]	陈美庆, 张养东, 郑楠, 等.牛奶中脂肪酸的合成机理及影响因素研究进展[J].动物营养学报, 2021, 33(8):4244-4254.CHEN M Q, ZHANG Y D, ZHENG N, et al.Advance in synthesis mechanism and influencing factors of milk fatty acids[J].Chinese Journal of Animal Nutrition, 2021, 33(8):4244-4254.(in Chinese)
[8]	MANGWE M, BRYANT R, GREGORINI P.Rumen fermentation and fatty acid composition of milk of mid lactating dairy cows grazing chicory and ryegrass[J].Animals (Basel), 2020, 10(1):169.
[9]	SOULAT J, KNAPP E, MOULA N, et al.Effect of dry-period diet on the performance and metabolism of dairy cows in early lactation[J].Animals (Basel), 2020, 10(5):803.
[10]	ZANFERARI F, VENDRAMINI T H A, RENTAS M F, et al.Effects of chitosan and whole raw soybeans on ruminal fermentation and bacterial populations, and milk fatty acid profile in dairy cows[J].J Dairy Sci, 2018, 101(12):10939-10952.
[11]	KAY J K, WEBER W J, MOORE C E, et al.Effects of week of lactation and genetic selection for milk yield on milk fatty acid composition in Holstein cows[J].J Dairy Sci, 2005, 88(11):3886-3893.
[12]	樊永亮, 张成龙, 张少卿, 等.中国荷斯坦牛一个完整泌乳期中乳脂肪酸变化规律研究[J].黑龙江畜牧兽医, 2016(8):95-97, 101.FAN Y L, ZHANG C L, ZHANG S Q, et al.Study on the change regularity of milk fatty acids in southern Holstein cattle in a complete lactation period[J].Heilongjiang Animal Science and Veterinary Medicine, 2016(15):95-97, 101.(in Chinese)
[13]	陈永华, 毛永江, 常玲玲.中国荷斯坦牛初乳、常乳与乳房炎乳乳成分及理化性质的比较研究[J].饲料广角, 2011(14):35-37.CHEN Y H, MAO Y J, CHANG L L.The Comparasion of milk composition and physical characteristics among colostrum, normal milk and mastitis of Chinese Holstein Cattle[J].Feed China, 2011(14):35-37.(in Chinese)
[14]	雷晓薇, 王根林, 韩兆玉.应用体细胞计数监测奶牛隐性乳房炎[J].畜牧与兽医, 2003, 35(12):35-37.LEI X W, WANG G L, HAN Z Y.Prediction of subclinical mastitis of dairy cows using milk somatic cell count[J].Animal Husbandry & Veterinary Medicine, 2003, 35(12):35-37.(in Chinese)
[15]	WANG J X, HE Y T, PANG K, et al.Changes in milk yield and composition of colostrum and regular milk from four buffalo breeds in China during lactation[J].J Sci Food Agric, 2019, 99(13):5799-5807.
[16]	张慧敏, 王梦琦, 朱小瑞, 等.中国荷斯坦牛乳中尿素氮变化规律的研究[J].家畜生态学报, 2016, 37(11):36-41.ZHANG H M, WANG M Q, ZHU X R, et al.Study on variability of milk urea nitrogen of Chinese Holstein[J].Journal of Domestic Animal Ecology, 2016, 37(11):36-41.(in Chinese)
[17]	BIONAZ M, LOOR J.ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation[J].J Nutr, 2008, 138(6):1019-1024.
[18]	高学军, 徐诗瑶, 王小艳, 等.泌乳中期不同乳品质奶牛乳中脂肪酸含量差异分析[J].东北农业大学学报, 2013, 44(9):7-11.GAO X J, XU S Y, WANG X Y, et al.Difference analysis of fatty acid contents on milk from different qualities of cows in mid-lactation period[J].Journal of Northeast Agricultural University, 2013, 44(9):7-11.(in Chinese)
[19]	BELIDE S, SHRESTHA P, KENNEDY Y, et al.Engineering docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in Brassica juncea[J].Plant Biotechnol J, 2022, 20(1):19-21.
[20]	付力立.基于代谢组学研究奶牛初乳与常乳组分差异及补饲酵母硒的影响[D].雅安:四川农业大学, 2019.FU L L.Differential metabolome between colostrum and normal milk in dairy cows and effects of supplementary yeast selenium-based on metabonomics[D].Ya'an:Sichuan Agricultural University, 2019.(in Chinese)
[21]	DERAKHSHANI H, TUN H M, CARDOSO F C, et al.Linking peripartal dynamics of ruminal microbiota to dietary changes and production parameters[J].Front Microbiol, 2017, 7:2143.
[22]	高凤.奶牛肠道微生物群落结构与多样性研究[D].邯郸:河北工程大学, 2017.GAO F.The study on intestinal microbial community structure and diversity of dairy cows[D].Handan:Hebei University of Engineering, 2017.(in Chinese)
[23]	BACH A, LÓPEZ-GARCÍA A, GONZÁLEZ-RECIO O, et al.Changes in the rumen and colon microbiota and effects of live yeast dietary supplementation during the transition from the dry period to lactation of dairy cows[J].J Dairy Sci, 2019, 102(7):6180-6198.
[24]	TONG J J, ZHANG H, YANG D L, et al.Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows[J].PLoS One, 2018, 13(11):e0198225.
[25]	JAMI E, WHITE B A, MIZRAHI I.Potential role of the bovine rumen microbiome in modulating milk composition and feed efficiency[J].PLoS One, 2014, 9(1):e85423.
[26]	WANG Y P, NAN X M, ZHAO Y G, et al.Ruminal degradation of rumen-protected glucose influences the ruminal microbiota and metabolites in early-lactation dairy cows[J].Appl Environ Microbiol, 2021, 87(2):e01908-20.
[27]	刘可园.奇数和支链脂肪酸与奶牛瘤胃微生物及其发酵指标的关系[D].哈尔滨:东北农业大学, 2016.LIU K Y.Odd-and Branched-Chain Fatty Acids profilesin relation to rumen microbial populations and fermentation patterns in dairy cows[D].Harbin:Northeast Agricultural University, 2016.(in Chinese)
[28]	SAWANON S, KOIKE S, KOBAYASHI Y.Evidence for the possible involvement of Selenomonas ruminantium in rumen fiber digestion[J].FEMS Microbiol Lett, 2011, 325(2):170-179.
[29]	王晓旭, 赵晨旭, 陈灰, 等.围产期奶牛瘤胃微生物区系的变化及其对瘤胃挥发性脂酸含量的影响[C]//中国畜牧兽医学会——第三届中国兽医临床大会论文集.兰州:中国畜牧兽医学会, 2012:106-110.WANG X X, ZHAO C X, CHEN H, et al.Correlation between composition of the bacterial community and concentration of volatile fatty acids in the rumen during the transition period and ketosis in dairy cows[C]//Proceedings of the 3rd Chinese Veterinary Clinical Congress of Chinese Association of Animal Husbandry and Veterinary Medicine.Lanzhou:Chinese Association of Animal Science and Veterinary Medicine, 2012:106-110.(in Chinese)
[30]	CERSOSIMO L M, BAINBRIDGE M L, KRAFT J, et al.Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows[J].BMC Microbiol, 2016, 16:78.
[31]	ZHAO L P, MENG Q X, REN L P, et al.Effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance[J].Asian-Australas J Anim Sci, 2015, 28(10):1433-1441.
[32]	AUFFRET M D, STEWART R D, DEWHURST R J, et al.Identification of microbial genetic capacities and potential mechanisms within the rumen microbiome explaining differences in beef cattle feed efficiency[J].Front Microbiol, 2020, 11:1229.
[33]	HUANG S, JI S K, WANG F R, et al.Dynamic changes of the fecal bacterial community in dairy cows during early lactation[J].AMB Expr, 2020, 10(1):167.
[34]	BUITENHUIS B, LASSEN J, NOEL S J, et al.Impact of the rumen microbiome on milk fatty acid composition of Holstein cattle[J].Genet Sel Evol, 2019, 51(1):23.
[35]	VLAEMINCK B, FIEVEZ V, CABRITA A R J, et al.Factors affecting odd- and branched-chain fatty acids in milk:a review[J].Anim Feed Sci Technol, 2006, 131(3-4):389-417.
[36]	LIU C, WU H, LIU S J, et al.Dynamic alterations in yak rumen bacteria community and metabolome characteristics in response to feed type[J].Front Microbiol, 2019, 10:1116.
[37]	LIU K Y, LI Y, LUO G B, et al.Relations of ruminal fermentation parameters and microbial matters to odd- and branched-chain fatty acids in rumen fluid of dairy cows at different milk stages[J].Animals (Basel), 2019, 9(12):1019.
[38]	KENNELLY J J, ROBINSON B, KHORASANI G R.Influence of carbohydrate source and buffer on rumen fermentation characteristics, milk yield, and milk composition in early-lactation Holstein cows[J].J Dairy Sci, 1999, 82(11):2486-2496.
[39]	DEWANCKELE L, TORAL P G, VLAEMINCK B, et al.Invited review:role of rumen biohydrogenation intermediates and rumen microbes in diet-induced milk fat depression:an update[J].J Dairy Sci, 2020, 103(9):7655-7681.
[40]	HUWS S A, KIM E J, LEE M R F, et al.As yet uncultured bacteria phylogenetically classified as Prevotella, Lachnospiraceae incertae sedis and unclassified Bacteroidales, Clostridiales and Ruminococcaceae may play a predominant role in ruminal biohydrogenation[J].Environ Microbiol, 2011, 13(6):1500-1512.

Changes of Rumen Microbe and Milk Fatty Acid Composition during Early Lactation

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