HMGB1与日本血吸虫病肝炎症和纤维化的相关性分析

doi:10.11843/j.issn.0366-6964.2022.10.032

Abstract

Abstract: To investigate the correlation of high mobility group box 1 (HMGB1) with liver inflammatory fibrosis induced by Schistosoma japonicum infection, in the present study, BALB/c mice were randomly divided into control group (n=10), 4 weeks' infection group, 6 weeks' infection group and HMGB1 inhibited group (n=5). Blood routine tests and biochemical tests were performed as well as liver pathology observation and Masson staining. qRT-PCR was used to detect the transcription levels of HMGB1,IL-1β,TNF-α,IL-6,IFN-γ,α-SMA,Col1α1,TGF-β1,Smad2 and Smad3 in the liver. Results showed that 4 weeks after infection, serum alanine aminotransferase (ALT) and the transcription levels of HMGB1, α-SMA, TGF-β1, IL-6 and TNF-α were increased significantly (P<0.05 or P<0.01); 6 weeks after infection, WBC, Neu, Mon, Eos, aspartate aminotransferase and ALT were significantly increased (P<0.01), the transcription levels of HMGB1, IL-1β, TNF-α, IL-6, IFN-γ, α-SMA, Col1α1, TGF-β1, Smad2 and Smad3 were significantly increased as well (P<0.05 or P<0.01); 6 weeks after infection, large number of eggs were deposited in the liver accompanied with neutrophils infiltration and collagen fiber accumulation. The HMGB1 inhibitor could significantly reduce the ALT level and transcription levels of HMGB1, α-SMA, TGF-β1, IL-6 and TNF-α in the liver (P<0.05). In conclusion, there was a correlation between HMGB1 and liver inflammatory fibrosis induced by S. japonicum infection.

Key words: Schistosoma japonicum, liver fibrosis, inflammation, high mobility group box 1

CLC Number:

ZHONG Haoran, HOU Ling, GUI Xiang, LÜ Rongxue, LIU Jinming, GU Shaopeng, JIN Yamei. The Correlation of HMGB1 with Liver Inflammatory Fibrosis Induced by Schistosoma japonicum[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3609-3620.

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The Correlation of HMGB1 with Liver Inflammatory Fibrosis Induced by Schistosoma japonicum

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