新型鹅细小病毒感染鸭的肝、胸腺、回肠转录组差异表达分析

doi:10.11843/j.issn.0366-6964.2022.02.032

Abstract

Abstract: This experiment was conducted to explore the molecular pathogenic mechanism of the interaction between novel goose parvovirus (NGPV) and host cells. In this study, transcriptome sequencing was performed on the liver, thymus and ileum of ducklings 14 days after infection with NGPV SD strain. The results showed that there were 78 up-regulated genes and 31 down-regulated genes in the ileum of the infected group; 111 up-regulated genes and 78 down-regulated genes in the thymus of the infected group; 128 up-regulated genes and 313 down-regulated genes in the liver of the infected group. The differentially expressed genes in thymus, ileum and liver of ducklings infected with NGPV are involved in many immune related biological processes, such as immune response process and lymphocyte mediated immunity. The differential genes are enriched in multiple signaling pathways such as Toll-like receptor signaling pathway, JAK-STAT signaling pathway, NF-κB signaling pathway, PI3K-Akt signaling. It is suggested that NGPV infection activates the body's immune response and the relevant antiviral signal pathways of host cells. This study provides new clues for the study of the pathogenic mechanism of NGPV.

Key words: novel goose parvovirus, short beak and dwarfism syndrome, transcriptome sequencing, immune response

CLC Number:

S858.325.3

WANG Luyao, HAO Xuepiao, LEI Baishi, ZHAO Kuan, ZHANG Wuchao, YUAN Wanzhe. Differential Expression of Transcriptome in Liver, Thymus and Ileum of Ducks Infected with Novel Goose Parvovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 654-657.

References 16

[1]	CHEN H, DOU Y G, TANG Y, et al. Isolation and genomic characterization of a duck-origin GPV-related parvovirus from Cherry Valley ducklings in China[J]. PLoS One, 2015, 10(10):e0140284.
[2]	LI P, LIN S, ZHANG R, et al. Isolation and characterization of novel goose parvovirus-related virus reveal the evolution of waterfowl parvovirus[J]. Transbound Emerg Dis, 2018, 65(2):e284-e295.
[3]	PALYA V, ZOLNAI A, BENYEDA Z, et al. Short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus[J]. Avian Pathol, 2009, 38(2):175-180.
[4]	CHEN S L, WANG S, CHENG X X, et al. Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China[J]. Arch Virol, 2016, 161(9):2407-2416.
[5]	KAPGATE S S, KUMANAN K, VIJAYARANI K, et al. Avian parvovirus:classification, phylogeny, pathogenesis and diagnosis[J]. Avian Pathol, 2018, 47(6):536-545.
[6]	刘荣昌, 黄瑜, 卢荣辉, 等. "短喙侏儒综合征"半番鸭病原学检测及病理组织学特征[J]. 中国兽医学报, 2018, 38(1):51-58.LIU R C, HUANG Y, LU R H, et al. Aetiology detection and histopathological features of "short beak dwarf syndrome" in mule ducks[J]. Chinese Journal of Veterinary Science, 2018, 38(1):51-58. (in Chinese)
[7]	LIU H M, YANG C C, LIU M M, et al. Pathological lesions in the immune organs of ducklings following experimental infection with goose parvovirus[J]. Res Vet Sci, 2019, 125:212-217.
[8]	陈兵, 徐婧, 罗启慧, 等. 感染鸭源小鹅瘟病毒QH-L01株樱桃谷雏鸭病理学研究[J]. 浙江农业学报, 2018, 30(6):932-938.CHEN B, XU J, LUO Q H, et al. Pathological study on duck-origin goose Parvovirus strain QH-L01 infection of Cherry Valley duck[J]. Acta Agriculturae Zhejiangensis, 2018, 30(6):932-938. (in Chinese)
[9]	MOGENSEN T H. Pathogen recognition and inflammatory signaling in innate immune defenses[J]. Clin Microbiol Rev, 2009, 22(2):240-273.
[10]	CHEN S L, FANG T H, XIAO S F, et al. Duckling short beak and dwarfism syndrome virus infection activates host innate immune response involving both DNA and RNA sensors[J]. Microb Pathog, 2020, 138:103816.
[11]	LIAO X Z, XIE H, LI S L, et al. 2', 5'-oligoadenylate synthetase 2(OAS2) inhibits Zika virus replication through activation of type Ι IFN signaling pathway[J]. Viruses, 2020, 12(4):418.
[12]	昝晓燕, 吴贻刚. 趋化因子配体19和趋化因子受体7在肥胖脂肪组织慢性炎症中的作用及有氧运动对其影响的研究进展[J]. 吉林大学学报:医学版, 2017, 43(3):659-662.ZAN X Y, WU Y G. Research progress in role of CCL19 and CCR7 in adipose tissue chronic inflammation and influence of aerobic exercise in CCL19-CCR signal[J]. Journal of Jilin University:Medicine Edition, 2017, 43(3):659-662.(in Chinese)
[13]	任乐豪. 甲型H1N1流感病毒激活宿主HIF-1信号通路促进病毒自身复制的机制研究[D]. 上海:上海交通大学, 2019.REN L H.Study on the mechanism of activating host HIF-1 signaling pathway to promote virus replication by H1N1 virus[D].Shanghai:Shanghai Jiaotong University,2019.(in Chinese)
[14]	DUETTE G, GERBER P P, RUBIONE J, et al. Induction of HIF-1α by HIV-1 infection in CD4+ T cells promotes viral replication and drives extracellular vesicle-mediated inflammation[J]. mBio, 2018, 9(5):e00757-18.
[15]	HSIEH Y C, CHEN Y M, LI C Y, et al. To complete its replication cycle, a shrimp virus changes the population of long chain fatty acids during infection via the PI3K-Akt-mTOR-HIF1α pathway[J]. Dev Comp Immunol, 2015, 53(1):85-95.
[16]	MENG X H, YANG D R, YU R, et al. EPSTI1 is involved in IL-28A-mediated inhibition of HCV infection[J]. Mediators Inflamm, 2015, 2015:716315.

Differential Expression of Transcriptome in Liver, Thymus and Ileum of Ducks Infected with Novel Goose Parvovirus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 16

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Xin, NIE Tong, LI Aqun, MA Jun. Hesperidin Alleviates High-fat-diet Induced Hepatic Oxidative Stress in Mice via Oxidative Phosphorylation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1302-1313.
[2]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[3]	HU Ting, ZHANG Yonghong, HOU Xiaolin, YAO Hua, CUI Defeng, PAN Zaozao, ZHANG Lingyu, ZHANG Jiaxi, WU Qiong. The Effects of Bisphenol A on Inflammation and Amino Acid Metabolism Pathways in Porcine Testis Sertoli Cells Based on Transcriptome Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2858-2871.
[4]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[5]	LIU Yuanyi, LI Xinyu, Bayinnamula, CUI Fang, MANG Lai, DU Ming. Single-Cell Transcriptome Sequencing Technology and its Application in Animal Reproduction [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 421-433.
[6]	LI Xiaobo, LIU Zhanfa, LIU Yue, CHEN Qian, MA Yuehui, ZHAO Qianjun, YE Shaohui. Mining Genes Related to Wool Bending of Zhongwei Goat Based on WGCNA and GSEA [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2930-2943.
[7]	WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.
[8]	MAO Yanni, CHANG Jiawei, LI Na, WANG Xin, KANG Xinyun, MA Qiang, MA Liang, WANG Guiqin. Transcriptome Differential Expression Analysis of Staphylococcus aureus in Biofilm State and Planktonic State [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2697-2707.
[9]	ZHAO Xuyang, JIN Jiaxin, LU Wenlong, ZHANG Shuai, HUANG Li, ZHANG Gaiping, SUN Aijun, ZHUANG Guoqing. Advances in the Molecular Mechanism of Immune Escape of African Swine Fever Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2074-2082.
[10]	ZHANG Na, WANG Fei, GE Ximin, ZHAO Guiping, WEN Jie, LI Qinghe. Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2396-2402.
[11]	LUO Jinhong, CHEN Xiang, SHANG Yishun, AO Ye, LI Pengcheng. Transcriptome Sequencing Screening the Genes Related to Goat Embryo Attachment in Early Pregnancy [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1465-1474.
[12]	LI Jie, ZHAO Ruipeng, CHEN Chuwen, YANG Chaowu, WU Jinbo, LI Zhixiong. Screening of the Differentially Expressed mRNA and lncRNA and the Construction of Their Competitive Regulatory Network in Embryonic Leg Muscles of Different Chicken Breeds [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4207-4220.
[13]	YIN Lei, PAN Xiaocheng, SHEN Xuehuai, ZHANG Danjun, DAI Yin, WANG Jieru. Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4527-4534.
[14]	TIAN Yaqing, CUI Lixin, HAO Haisheng, ZOU Huiying, PANG Yunwei, ZHAO Xueming, ZHU Huabin, DU Weihua. Effects of ASH1L Knockdown on mRNA Profiling in Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 151-160.
[15]	ZHU Jingjing, DAI Zhenglie, WANG Han, LI Xiangchen, ZHAO Ayong, ZHOU Xiaolong, YANG Songbai. Analysis of Differential Expression Profile of LncRNA in PK15 Cells Infected with Japanese Encephalitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 272-281.