乙型脑炎病毒感染PK15细胞的lncRNA差异表达谱分析

doi:10.11843/j.issn.0366-6964.2022.01.027

Abstract

Abstract: The objective of this study was to analyze the differential expression profile of lncRNA in pig kidney epithelial cells PK15 infected by Japanese encephalitis virus (JEV), and to explore the potential function of host lncRNA in JEV infection. In this study, JEV was used to infect PK15 cells for 36 hours, and the cells were collected. Meanwhile, an uninfected control group was established. There were 3 biological replicates in each group. The total RNA was extracted, and the cDNA libraries were constructed and then subjected to high-throughput sequencing to identify differentially expressed lncRNA. The cis- and trans-acting target genes of lncRNA were obtained by calculating the positional relationship and sequence similarity between differentially expressed lncRNA and mRNA. Next, the predicted target genes were analyzed by GO and KEGG. Finally, 9 differentially expressed lncRNAs were randomly selected for expression verification using real-time fluorescent quantitative PCR (quantitative reverse transcription PCR, qRT-PCR) technology. The results showed that a total of 856 differentially expressed lncRNAs were identified, of which 452 lncRNAs were significantly up-regulated and 404 lncRNAs were significantly down-regulated. GO analysis results showed that the target genes of lncRNAs were mainly enriched in innate immune response; KEGG pathway analysis results showed that the target genes of lncRNA were significantly enriched in signal pathways such as tumor necrosis factor, Toll-like receptor and NF-κB. The quantitative results are the same as the sequencing results through qRT-PCR verification. This study lays a theoretical foundation for further exploring the roles of lncRNA in JEV infection.

Key words: swine, Japanese encephalitis virus, innate immune response, PK15 cells, high-throughput sequencing

CLC Number:

S855.3

ZHU Jingjing, DAI Zhenglie, WANG Han, LI Xiangchen, ZHAO Ayong, ZHOU Xiaolong, YANG Songbai. Analysis of Differential Expression Profile of LncRNA in PK15 Cells Infected with Japanese Encephalitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 272-281.

References 38

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Analysis of Differential Expression Profile of LncRNA in PK15 Cells Infected with Japanese Encephalitis Virus

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