鸡毒支原体MG-HY株感染鸡气管的转录组学分析

doi:10.11843/j.issn.0366-6964.2022.08.023

Abstract

Abstract: To further explore the changes of transcription level of tracheal genome after Mycoplasma gallisepticum (MG) infection in SPF chickens, the differentially expressed genes and regulatory pathways involved in inflammatory injury of tracheal mucosa after MG infection were screened; The infection model was established by infusing SPF chickens with MG-HY strain at 0.2 mL per feather through nasal and eye drops. Tracheal tissues were collected and then using RNA-Seq technology for sequencing analysis. The results of transcriptome analysis showed that a total of 3 112 significantly (P<0.01) differentially expressed genes (DEGs) were selected by RNA-seq during MG infection, including 1 646 up-regulated genes and 1 466 down-regulated genes. GO functional analysis found that differential genes mainly involve biological processes such as biological regulation, stimulus response, multicellular biological processes, cell component organization or biogenesis. KEGG-Pathway analysis showed that differential genes are involved in mucosal immune signaling pathways, such as:cytokine-cytokine receptor interactions, cell adhesion molecules (CAMs), extracellular matrix (ECM) receptor interactions, tight junctions, PPAR signaling pathways and MAPK signaling pathway, etc., overall, these results showed that these genes may be involved in MG-induced tracheal inflammatory response and injury in chicks. Using qRT-PCR to verify 13 differentially expressed genes related to mucosal immunity, the results are consistent with transcriptome analysis. This study provides a basis for further elucidating the host tracheal mucosal epithelial damage and mucosal immune mechanism caused by MG infection.

Key words: Mycoplasma gallisepticum, RNA-seq, mucosal epithelium, differentially expressed gene, immune response

CLC Number:

S852.62

WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.

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Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection

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