鸡毒支原体MG-HY株感染鸡气管的转录组学分析

doi:10.11843/j.issn.0366-6964.2022.08.023

Abstract

Abstract: To further explore the changes of transcription level of tracheal genome after Mycoplasma gallisepticum (MG) infection in SPF chickens, the differentially expressed genes and regulatory pathways involved in inflammatory injury of tracheal mucosa after MG infection were screened; The infection model was established by infusing SPF chickens with MG-HY strain at 0.2 mL per feather through nasal and eye drops. Tracheal tissues were collected and then using RNA-Seq technology for sequencing analysis. The results of transcriptome analysis showed that a total of 3 112 significantly (P<0.01) differentially expressed genes (DEGs) were selected by RNA-seq during MG infection, including 1 646 up-regulated genes and 1 466 down-regulated genes. GO functional analysis found that differential genes mainly involve biological processes such as biological regulation, stimulus response, multicellular biological processes, cell component organization or biogenesis. KEGG-Pathway analysis showed that differential genes are involved in mucosal immune signaling pathways, such as:cytokine-cytokine receptor interactions, cell adhesion molecules (CAMs), extracellular matrix (ECM) receptor interactions, tight junctions, PPAR signaling pathways and MAPK signaling pathway, etc., overall, these results showed that these genes may be involved in MG-induced tracheal inflammatory response and injury in chicks. Using qRT-PCR to verify 13 differentially expressed genes related to mucosal immunity, the results are consistent with transcriptome analysis. This study provides a basis for further elucidating the host tracheal mucosal epithelial damage and mucosal immune mechanism caused by MG infection.

Key words: Mycoplasma gallisepticum, RNA-seq, mucosal epithelium, differentially expressed gene, immune response

CLC Number:

S852.62

WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.

References 32

[1]	BOWER K, DJORDJEVIC S P, ANDRONICOS N M, et al. Cell surface antigens of Mycoplasma species bovine group 7 bind to and activate plasminogen[J]. Infect Immun, 2003, 71(8):4823-4827.
[2]	丁亮. 鸡毒支原体F弱毒疫苗对肉鸡生长及免疫性能影响的研究[D]. 合肥:安徽农业大学, 2011.DING L. The effect of F strain vaccine of M. gallisepticum on growth performance and immunity of broiler[D]. Hefei:Anhui Agricultural University, 2011. (in Chinese)
[3]	陈继荣. 临床分离鸡毒支原体pvpA基因的分子特征及其原核表达[D]. 广州:华南农业大学, 2008.CHEN J R. Molecular characterization of adhesin-encoding gene pvpA among isolated Mycoplasma gallisepticum and prokaryotic expression of PvpA[D]. Guangzhou:South China Agricultural University, 2008. (in Chinese)
[4]	彭秀丽. 鸡毒霉形体黏附素特性鉴定及其鸡胚组织互作蛋白的分布研究[D]. 武汉:华中农业大学, 2008.PENG X L. Characteristic identification of Mycoplasma gallisepticum adhesin protein and the distribution of its interactive proteins in chick embryo tissues[D]. Wuhan:Huazhong Agricultural University, 2008. (in Chinese)
[5]	郝卫芳. 鸡毒霉形体HS株TM-1和mgc3基因的表位预测及原核表达[D]. 武汉:华中农业大学, 2007.HAO W F. Epitope prediction and prokaryotic expression of genes of TM-1 & mgc3 of Mycoplasma gallisepticum HS strain[D]. Wuhan:Huazhong Agricultural University, 2007. (in Chinese)
[6]	JAVED M A, FRASCA S Jr, ROOD D. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow[J]. Infect Immun, 2005, 73(9):5410-5419.
[7]	MAJUMDER S, ZAPPULLA F, SILBART L K. Mycoplasma gallisepticum lipid associated membrane proteins up-regulate inflammatory genes in chicken tracheal epithelial cells via TLR-2 ligation through an NF-κB dependent pathway[J]. PLoS One, 2014, 9(11):e112796.
[8]	BURGESS D J. A space for transcriptomics[J]. Nat Rev Genet, 2016, 17(8):436-437.
[9]	林琳. 闽北地区白羽肉鸡鸡毒支原体感染的病原学调查及其流行菌株的分离鉴定[D]. 福州:福建农林大学, 2018.LIN L. Etiological investigation of M. gallisepticum in white meat-type chickens in northern Fujian province and isolation and identification of epidemic strains[D]. Fuzhou:Fujian Agricultural and Forestry University, 2018. (in Chinese)
[10]	ZHANG D C, LONG Y Q, LI M, et al. Development and evaluation of novel recombinant adenovirus-based vaccine candidates for infectious bronchitis virus and Mycoplasma gallisepticum in chickens[J]. Avian Pathol, 2018, 47(2):213-222.
[11]	ZHANG W, LIU Y H, ZHANG Q M, et al. Mycoplasma gallisepticum infection impaired the structural integrity and immune function of bursa of fabricius in chicken:implication of oxidative stress and apoptosis[J]. Front Vet Sci, 2020, 7:225.
[12]	AIRD D, ROSS M G, CHEN W S, et al. Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries[J]. Genome Biol, 2011, 12(2):R18.
[13]	WANG L K, FENG Z X, WANG X, et al. DEGseq:an R package for identifying differentially expressed genes from RNA-seq data[J]. Bioinformatics, 2010, 26(1):136-138.
[14]	ANDERS S, HUBER W. Differential expression analysis for sequence count data[J]. Genome Biol, 2010, 11(10):R106.
[15]	MORIYA Y, ITOH M, OKUDA S. KAAS:an automatic genome annotation and pathway reconstruction server[J]. Nucleic Acids Res, 2007, 35(Web Server issue):W182-W185.
[16]	施少华, 陈珍, 程龙飞, 等. 鸭源H7N9亚型禽流感病毒感染SPF鸡转录组学分析[J]. 中国人兽共患病学报, 2020, 36(11):886-893.SHI S H, CHEN Z, CHENG L F, et al. Transcriptomic expression profiles of SPF chicken infected with duck-origin H7N9 subtype avian influenza virus[J]. Chinese Journal of Zoonoses, 2020, 36(11):886-893. (in Chinese)
[17]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4):402-408.
[18]	孟冬霞, 贺东昌, 武果桃, 等. 鸡毒支原体感染病理模型复制[J]. 中国兽药杂志, 2012, 46(10):36-38.MENG D X, HE D C, WU G T, et al. Establishment of pathology model of Mycoplasma gallisepticum infection[J]. Chinese Journal of Veterinary Drug, 2012, 46(10):36-38. (in Chinese)
[19]	SID H, HARTMANN S, PETERSEN H, et al. Mycoplasma gallisepticum modifies the pathogenesis of influenza A virus in the avian tracheal epithelium[J]. Int J Med Microbiol, 2016, 306(3):174-186.
[20]	吴春琳, 黄宝钦, 蓝天韵, 等. 福建白羽肉鸡鸡毒支原体的分离鉴定及最小抑菌浓度测定[J]. 中国畜牧兽医, 2021, 48(4):1489-1497.WU C L, HUANG B Q, LAN T Y, et al. Isolation, identification and minimum inhibitory concentration determination of Mycoplasma gallisepticum from white-feathered broilers in Fujian[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(4):1489-1497. (in Chinese)
[21]	KORYTINA G F, AKHMADISHINA L Z, KOCHETOVA O V, et al. The role of serum amyloid A1, adhesion molecules, chemokines, and chemokine receptors genes in chronic obstructive pulmonary disease[J]. Russ J Genet, 2019, 55(1):105-113.
[22]	YU Y, CHEN Y, WANG Y, et al. TLR2/MyD88/NF-κB signaling pathway regulates IL-1β production in DF-1 cells exposed to Mycoplasma gallisepticum LAMPs[J]. Microb Pathog, 2018, 117:225-231.
[23]	SWART G W M. Activated leukocyte cell adhesion molecule (CD166/ALCAM):developmental and mechanistic aspects of cell clustering and cell migration[J]. Eur J Cell Biol, 2002, 81(6):313-321.
[24]	SAMANTA D, ALMO S C. Nectin family of cell-adhesion molecules:structural and molecular aspects of function and specificity[J]. Cell Mol Life Sci, 2015, 72(4):645-658.
[25]	NI L G, SONG C Y, WU X S, et al. RNA-seq transcriptome profiling of porcine lung from two pig breeds in response to Mycoplasma hyopneumoniae infection[J]. PeerJ, 2019, 7:e7900.
[26]	MOHAMMED J, FRASCA S Jr, CECCHINI K, et al. Chemokine and cytokine gene expression profiles in chickens inoculated with Mycoplasma gallisepticum strains Rlow or GT5[J]. Vaccine, 2007, 25(51):8611-8621.
[27]	JAHEJO A R, NIU S, ZHANG D. Transcriptome analysis of MAPK signaling pathway and associated genes to angiogenesis in chicken erythrocytes on response to thiram-induced tibial lesions[J]. Res Vet Sci, 2019, 127:65-75.
[28]	QU J R, WANG W, ZHANG Q J, et al. Inhibition of lipopolysaccharide-induced inflammation of chicken liver tissue by selenomethionine via TLR4-NF-κB-NLRP3 signaling pathway[J]. Biol Trace Elem Res, 2020, 195(1):205-214.
[29]	ZOU M Y, YANG W L, NIU L M, et al. Polydatin attenuates Mycoplasma gallisepticum (HS strain)-induced inflammation injury via inhibiting the TLR6/MyD88/NF-κB pathway[J]. Microb Pathog, 2020, 149:104552.
[30]	BEAUDET J, TULMAN E R, PFLAUM K, et al. Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum[J]. Infect Immun, 2019, 87(3):e00613-18.
[31]	DAI C, BASILICO P, CREMONA T P, et al. CXCL14 displays antimicrobial activity against respiratory tract bacteria and contributes to clearance of Streptococcus pneumoniae pulmonary infection[J]. J Immunol, 2015, 194(12):5980-5989.
[32]	MAERKI C, MEUTER S, LIEBI M, et al. Potent and broad-spectrum antimicrobial activity of CXCL14 suggests an immediate role in skin infections[J]. J Immunol, 2009, 182(1):507-514.

Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection

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