鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2022.12.038

Abstract

Abstract: The aim of this study was to investigate the differential expression characteristics of miRNA in bone marrow of chicks infected with Salmonella enterica serovar Pullorum (S. Pullorum), thus to provide a theoretical basis for an in-depth understanding of the pathogenic mechanism of S. Pullorum. Seven-day-old SPF chicks were randomly divided into two groups and orally administered Salmonella enterica serovar Pullorum and PBS, respectively. Bone marrow was collected 24 h after infection for miRNA sequencing, and differentially expressed miRNAs with differential multiplicity ≥2 and P value ≤0.05 were screened for target gene prediction, GO and KEGG enrichment analysis, while six miRNAs of which were randomly selected for qRT-PCR validation. An miRNA-mRNA target network related to the immune process was constructed. A total of 20 known differentially expressed miRNAs were obtained by miRNA sequencing, of which 11 were up-regulated and 9 were down-regulated. qRT-PCR results showed that the miRNA change trend was consistent with the sequencing results. GO analysis showed that the differentially expressed genes were mainly enriched in membrane transport, signal transduction, immune system, carbohydrate metabolism, sugar biosynthesis and metabolism, etc. The signaling pathways of KEGG were mainly enriched in Notch signaling pathway, Hedgehog signaling pathway, PPAR signaling pathway, AMPK signaling pathway, Hippo signaling pathway, etc. The miRNA-mRNA network interactions identified gga-miR-1466 and gga-miR-6643-5p as possible key miRNAs involved in immune process. This experiment resolved the bone marrow miRNA expression profiles of S. Pullorum-infected chicks, providing new insights into the complex molecular pathogenesis of the interaction between S. Pullorum and chickens, and offering new strategies for the prevention and control of S. Pullorum infection in chickens.

Key words: Salmonella enterica serovar Pullorum, infection, bone marrow, miRNA, immune response

CLC Number:

S858.315.1

YIN Lei, PAN Xiaocheng, SHEN Xuehuai, ZHANG Danjun, DAI Yin, WANG Jieru. Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4527-4534.

References 23

[1]	王小纳.鸡白痢危害与预防措施[J].中国畜禽种业,2020,16(11):166.WANG X N.The Salmonella enterica serovar Pullorum hazards and preventive measures[J].The Chinese Livestock and Poultry Breeding,2020,16(11):166.(in Chinese)
[2]	ZHANG D,ZHUANG L L,WANG C M,et al.Virulence gene distribution of Salmonella Pullorum isolates recovered from chickens in China (1953-2015)[J].Avian Dis,2018,62(4):431-436.
[3]	BENATAR T,LACAMPO S,TKALEC L,et al.Expression of immunoglobulin genes in the avian embryo bone marrow revealed by retroviral transformation[J].Eur J Immunol,1991,21(10):2529-2536.
[4]	KONDO M,WEISSMAN I L,AKASHI K.Identification of clonogenic common lymphoid progenitors in mouse bone marrow[J].Cell,1997,91(5):661-672.
[5]	CIRACI C,TUGGLE C K,WANNEMUEHLER M J,et al.Unique genome-wide transcriptome profiles of chicken macrophages exposed to Salmonella-derived endotoxin[J].BMC Genomics,2010,11:545.
[6]	刘亚娟,保雨,彭祥伟,等.宿主miRNA调节沙门氏菌感染的研究进展[J].中国预防兽医学报,2015,37(12):973-977.LIU Y J,BAO Y,PENG X W,et al.Advances in the study of host miRNAs regulating Salmonella infection[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(12):973-977.(in Chinese)
[7]	宋祥军,邱明宇,蒋胡艳,等.禽致病性大肠杆菌(APEC)感染对雏鸡脾脏miRNA表达谱的影响[J].中国兽医学报,2019,39(12):2373-2379.SONG X J,QIU M Y,JIANG H Y,et al.Effect of APEC infection on miRNA expression profile of chick spleen[J].Chinese Journal of Veterinary Science,2019,39(12):2373-2379.(in Chinese)
[8]	SUN H Y,LIU P,NOLAN L K,et al.Avian pathogenic Escherichia coli (APEC) infection alters bone marrow transcriptome in chickens[J].BMC Genomics,2015,16(1):690.
[9]	HONG Y,TRUONG A D,LEE J,et al.Exosomal miRNA profiling from H5N1 avian influenza virus-infected chickens[J].Vet Res,2021,52(1):36.
[10]	LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method[J].Methods,2001,25(4):402-408.
[11]	YONEYAMA M,KIKUCHI M,NATSUKAWA T,et al.The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses[J].Nat Immunol,2004,5(7):730-737.
[12]	陈明杰,曹梦园,曹芳,等.miRNA对细菌性脑膜炎中血脑屏障影响的研究进展[J].现代畜牧兽医,2021(12):83-86.CHEN M J,CAO M Y,CAO F,et al.Research progress on the effect of miRNA on the blood-brain barrier in bacterial meningitis[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2021(12):83-86.(in Chinese)
[13]	苏莉,竺婷婷,师红铃,等.结核分枝杆菌感染中凋亡相关microRNA的研究进展[J].中国兽医学报,2021,41(7):1425-1430.SU L,ZHU T T,SHI H L,et al.Research progress of apoptosis-related microRNAs in Mycobacterium tuberculosis infection[J].Chinese Journal of Veterinary Science,2021,41(7):1425-1430.(in Chinese)
[14]	KILPINEN L,PARMAR A,GRECO D,et al.Expansion induced microRNA changes in bone marrow mesenchymal stromal cells reveals interplay between immune regulation and cell cycle[J].Aging,2016,8(11):2799-2813.
[15]	FAN Z Y,WANG Q M,DENG H.Circ_0011460 upregulates HTRA1 expression by sponging miR-762 to suppress HTR8/SVneo cell growth,migration,and invasion[J].Am J Reprod Immunol,2021,86(5):e13485.
[16]	ZHOU Y T,TIAN W H,ZHANG M,et al.Transcriptom analysis revealed regulation of dexamethasone induced microRNAs in chicken thymus[J].J Cell Biochem,2019,120(4):6570-6579.
[17]	IRIZARRY K J L,CHAN A,KETTLE D,et al.Bioinformatics analysis of chicken miRNAs associated with monocyte to macrophage differentiation and subsequent IFNγ stimulated activation[J].MicroRNA, 2017,6(1):53-70.
[18]	SI X M,CHEN J,HUANG L.RRV-induced biliary atresia in neonatal mice involves CD8+T lymphocyte killer cells and the Notch signaling pathway[J].Genes Genomics,2021,43(11):1289-1299.
[19]	BABAGANA M,OH K S,CHAKRABORTY S,et al.Hedgehog dysregulation contributes to tissue-specific inflammaging of resident macrophages[J].Aging,2021,13(15):19207-19229.
[20]	ISALI I,MCCLELLAN P,SHANKAR E,et al.Genipin guides and sustains the polarization of macrophages to the pro-regenerative M2 subtype via activation of the pSTAT6-PPAR-gamma pathway[J].Acta Biomater,2021,131:198-2103.
[21]	SAIKIA R,JOSEPH J.AMPK:a key regulator of energy stress and calcium-induced autophagy[J].J Mol Med (Berl),2021,99(11):1539-1551.
[22]	HE T S,DANG L L,ZHANG J H,et al.The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway[J].Cell Death Differ,2022,29(1):192-205.
[23]	LIU Y Y,ZHENG M,JIAO M Z,et al.Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy[J].Biomaterials,2021,276:121036.

Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 23

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Weiye, HUANG Xuewei. Research Progress of Non-coding RNA in Infectious Bursal Disease Virus Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1488-1498.
[2]	DU Gaimei, WANG Yue, MAO Huihua, LEI Weiqiang, CHU Yuefeng, LIU Maojun. Establishment of the Mice Model Infected with Mycoplasma ovipneumoniae [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1728-1737.
[3]	TIAN Qihui, ZHANG Liang, LONG Yali. Study on the Effect of Astragalus on Proliferation of Bone Marrow Mesenchymal Stem Cells in Anoxic Microenvironment based on PI3K-AKT Signal Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 346-354.
[4]	ZHANG Qianwen, LIU Yumei, SHI Lihui, LIANG Wenjun, LI Mengyun, WANG Yuqin, ZHANG Ziqiang. Pathological Observation and Drug Sensitivity Analysis of Salmonella Infection in Female Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3510-3518.
[5]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[6]	SUI Yuzhen, CHEN Guizhen, ZUO Shoujun, WANG Haidong, SONG Pengtao, LI Liangliang, ZHANG Longxian, DONG Haiju, LIU Fang. Analysis on Infection Status of Clonorchis sinensis and Other Intestinal Parasites in Pet Cats in Henan Province [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3022-3030.
[7]	MAO Peng, WANG Zhihao, LI Jianji, CUI Luying, ZHU Guoqiang, MENG Xia, DONG Junsheng, WANG Heng. Research Progress of Ferroptosis in Bacterial Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2280-2287.
[8]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[9]	SUN Yufan, YU Panyuan, CHEN Hongyu, TAN Yiqing, CHEN Xiabing, ZHANG Tengfei, GAO Ting, ZHOU Rui, LI Lu. Evaluation of the Efficacy of Potassium Diformate in the Prevention of Salmonella Infection and the Effect on Intestinal Flora [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2101-2113.
[10]	QIN Xue, SHA Yiwen, YANG Menghao, CAI Rui, PANG Weijun. Advances in Regulation of Non-coding RNA on Mammalian Endometrial Receptivity and Decidualization [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1347-1358.
[11]	HAN Lulu, HAN Deping, ZHAO Qinan, DIAO Qiyu, CUI Kai. Research Progress of Intestinal Injury in Young Farm Animals under Stress Mediated by miRNA [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 877-888.
[12]	JIANG Huihua, LI Ning, XU Lei, GUO Kangkang. Research Progress on the Role of Neutrophil Extracellular Traps in Pathogenic Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 889-899.
[13]	LI Huawei, WANG Xuying, QIAO Hongxing, LI Xinfeng, JI Xiangbo, GUO Kewei, YANG Zhongyuan. Establishment of a Lentiviral Vector-mediated Stable Expression of eIF5A Cell Line and Its Effect on Porcine Reproductive and Respiratory Syndrome Virus Propagation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1169-1176.
[14]	WANG Lan, HE Mingyu, ZHANG Min, DING Juntao. MicroRNAs Regulate Antiviral Immunity and Viral Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 463-472.
[15]	SHANG Jinyuan, YAN Manping, YE Jingfei, CHENG Yuening, WANG Zhenjun, FENG Erkai, WANG Chunxia, ZHAO Yan, ZHU Xianpeng, LIAO Yuanjun, LUO Guoliang. Research Progress on Virus Infection and Its Anti-tumor Mechanisms of Protoparvovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4551-4559.