非洲猪瘟血清学诊断靶点的研究进展

doi:10.11843/j.issn.0366-6964.2021.05.007

Abstract

Abstract: African swine fever (ASF) is a highly fatal infectious disease caused by African swine fever virus (ASFV) in pigs. The proteins p30, p54 and p72 encoded by ASFV have high immunogenicity and some amino acid sequences are conserved, so they are often used as serological diagnostic targets to evaluate the changes of antibody levels through different stages or degrees of ASFV infection. In-depth study of ASF serological diagnostic targets would be helpful to explore its infection detection, pathogenic mechanism and immune system response. This paper systematically reviewed the progress in the detection of main candidate proteins and their targets for serological diagnosis of ASF, analyzed the antigenic epitopes (regions) of candidate targets and the characteristics of antibody binding, and discussed their application potential as new targets for serological diagnosis of ASF.

Key words: African swine fever virus, diagnostic targets, structural proteins, antigenic epitopes

CLC Number:

S852.659.1

YANG Wenbing, ZOU Yawen, JIANG Yifan, YU Wanting, YANG Yi, WU Jing, WANG Naidong. Advances Research on African Swine Fever Serological Diagnostic Targets[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1208-1217.

References 68

[1]	GAUDREAULT N N, MADDEN D W, WILSON W C, et al. African swine fever virus: An emerging DNA arbovirus[J]. Front Vet Sci, 2020, 7:215.
[2]	REVILLA Y, PÉREZ-NÚÑEZ D, RICHT J A. African swine fever virus biology and vaccine approaches[J]. Adv Virus Res, 2018, 100:41-74.
[3]	DIXON L K, ISLAM M, NASH R, et al. African swine fever virus evasion of host defences[J]. Virus Res, 2019, 266:25-33.
[4]	PENRITH M L, VOSLOO W. Review of African swine fever: transmission, spread and control[J]. J S Afr Vet Assoc, 2009, 80(2):58-62.
[5]	MONTGOMERY R E. On a form of swine fever occur-ring in British East Africa (Kenya Colony)[J]. J Comp Pathol Therapeut, 1921, 34:159-191.
[6]	张睿, 黄旖童, 鲍晨沂, 等. 非洲猪瘟流行病学及其在中国扩散的因素分析[J]. 病毒学报, 2019, 35(3):512-522.ZHANG R, HUANG Y T, BAO C Y, et al. Epidemiology of African swine fever and analysis of risk factors of its spread in China: An overview[J]. Chinese Journal of Virology, 2019, 35(3):512-522. (in Chinese)
[7]	LADDOMADA A, ROLESU S, LOI F, et al. Surveillance and control of African swine fever in free-ranging pigs in Sardinia[J]. Transbound Emerg Dis, 2019, 66(3):1114-1119.
[8]	PETRINI S, FELIZIANI F, CASCIARI C, et al. Survival of African swine fever virus (ASFV) in various traditional Italian dry-cured meat products[J]. Prev Vet Med, 2019, 162:126-130.
[9]	GE S Q, LI J M, FAN X X, et al. Molecular characterization of African swine fever virus, China, 2018[J]. Emerg Infect Dis, 2018, 24(11):2131-2133.
[10]	ZHOU X T, LI N, LUO Y Z, et al. Emergence of African swine fever in China, 2018[J]. Transbound Emerg Dis, 2018, 65(6):1482-1484.
[11]	姜睿娇, 张鹏飞, 朱光恒, 等. 非洲猪瘟检测技术进展[J]. 病毒学报, 2019, 35(3):523-532.JIANG R J, ZHANG P F, ZHU G H, et al. Diagnostic assays for African swine fever[J]. Chinese Journal of Virology, 2019, 35(3):523-532. (in Chinese)
[12]	LI L, REN Z W, WANG Q H, et al. Infection of African swine fever in wild boar, China, 2018[J]. Transbound Emerg Dis, 2019, 66(3):1395-1398.
[13]	GOATLEY L C, REIS A L, PORTUGAL R, et al. A pool of eight virally vectored African swine fever antigens protect pigs against fatal disease[J]. Vaccines, 2020, 8(2):234.
[14]	WU K K, LIU J M, WANG L X, et al. Current state of global African swine fever vaccine development under the prevalence and transmission of ASF in China[J]. Vaccines, 2020, 8(3):531.
[15]	BELLINI S, RUTILI D, GUBERTI V. Preventive measures aimed at minimizing the risk of African swine fever virus spread in pig farming systems[J]. Acta Vet Scand, 2016, 58(1):82.
[16]	GALLARDO C, FERNÁNDEZ-PINERO J, ARIAS M. African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation[J]. Virus Res, 2019, 271:197676.
[17]	王西西. 非洲猪瘟病毒蛋白对cGAS-STING信号通路抑制作用研究[D]. 北京:中国农业科学院, 2019.WANG X X. Inhibition mechanisms of African swine fever virus protein on cGAS-STING-mediated signaling pathway[D]. Beijing:Chinese Academy of Agricultural Sciences, 2019. (in Chinese)
[18]	JIA N, OU Y W, PEJSAK Z, et al. Roles of African swine fever virus structural proteins in viral infection[J]. J Vet Res, 2017, 61(2):135-143.
[19]	LITHGOW P, TAKAMATSU H, WERLING D, et al. Correlation of cell surface marker expression with African swine fever virus infection[J]. Vet Microbiol, 2014, 168(2-4):413-419.
[20]	GÓMEZ-PUERTAS P, RODRÍGUEZ F, OVIEDO J M, et al. The African swine fever virus proteins p54 and p30 are involved in two distinct steps of virus attachment and both contribute to the antibody-mediated protective immune response[J]. Virology, 1998, 243(2):461-471.
[21]	BARDERAS M G, RODRÍGUEZ F, GÓMEZ-PUERTAS P, et al. Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins[J]. Arch Virol, 2001, 146(9):1681-1691.
[22]	RODRÍGUEZ J M, GARCÍA-ESCUDERO R, SALAS M L, et al. African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites[J]. J Virol, 2004, 78(8):4299-4313.
[23]	HERNÁEZ B, DÍAZ-GIL G, GARCÍA-GALLO M, et al. The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis[J]. FEBS Lett, 2004, 569(1-3):224-228.
[24]	ALCARAZ C, RODRIGUEZ F, OVIEDO J M, et al. Highly specific confirmatory western blot test for African swine fever virus antibody detection using the recombinant virus protein p54[J]. J Virol Methods, 1995, 52(1-2):111-119.
[25]	LIU Q, MA B T, QIAN N C, et al. Structure of the African swine fever virus major capsid protein p72[J]. Cell Res, 2019, 29(11):953-955.
[26]	NEILAN J G, ZSAK L, LU Z, et al. Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection[J]. Virology, 2004, 319(2):337-342.
[27]	RODRÍGUEZ J M, YÁÑEZ R J, ALMAZÁN F, et al. African swine fever virus encodes a CD2 homolog responsible for the adhesion of erythrocytes to infected cells[J]. J Virol, 1993, 67(9):5312-5320.
[28]	SANNA G, DEI GIUDICI S, BACCIU D, et al. Improved strategy for molecular characterization of African swine fever viruses from Sardinia, based on analysis of p30, CD2v and I73R/I329L variable regions[J]. Transbound Emerg Dis, 2017, 64(4):1280-1286.
[29]	RUIZ-GONZALVO F, RODRÍGUEZ F, ESCRIBANO J M. Functional and immunological properties of the baculovirus-expressed hemagglutinin of African swine fever virus[J]. Virology, 1996, 218(1):285-289.
[30]	周晓慧, 肖景景, 张鑫宇, 等. 非洲猪瘟病毒强免疫原性重组CD2v抗原的制备与初步应用[J]. 畜牧兽医学报, 2020, 51(10):2472-2480.ZHOU X H, XIAO J J, ZHANG X Y, et al. Preparation and preliminary application of highly immunogenic recombinant CD2v antigen of African swine fever virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10):2472-2480. (in Chinese)
[31]	钟秋萍, 于婉琪, 薄宗义, 等. 非洲猪瘟病毒外膜蛋白CD2v的原核表达及其免疫特性的研究[J]. 中国兽医科学, 2020, 50(6):689-695.ZHONG Q P, YU W Q, BO Z Y, et al. Prokaryotic expression and immunological characterization of African swine fever virus out membrane CD2v protein[J]. Chinese Veterinary Science, 2020, 50(6):689-695. (in Chinese)
[32]	于浩洋, 吴绍强, 王彩霞, 等. 非洲猪瘟CD2v胞外区基因片段的真核表达及其多克隆抗体的制备[J]. 中国兽医科学, 2021, 51(1):45-52.YU H Y, WU S Q, WANG C X, et al. Eukaryotic expression the CD2v extracellular gene fragment of African swine fever virus and characterization of its polyclonal antibodies[J]. Chinese Veterinary Science, 2021, 51(1):45-52. (in Chinese)
[33]	MONTEAGUDO P L, LACASTA A, LÓPEZ E, et al. BA71ΔCD2:a new recombinant live attenuated African swine fever virus with cross-protective capabilities[J]. J Virol, 2017, 91(21):e01058-17.
[34]	BORCA M V, CARRILLO C, ZSAK L, et al. Deletion of a CD2-like gene, 8-DR, from African swine fever virus affects viral infection in domestic swine[J]. J Virol, 1998, 72(4):2881-2889.
[35]	BERGERON H C, GLAS P S, SCHUMANN K R. Diagnostic specificity of the African swine fever virus antibody detection enzyme-linked immunosorbent assay in feral and domestic pigs in the United States[J]. Transbound Emerg Dis, 2017, 64(6):1665-1668.
[36]	STRIZHAKOVA O M, LYSKA V M, MALOGOLOVKIN A S, et al. Validation of an ELISA kit for detection of antibodies against:ASF virus in blood or spleen of domestic pigs and wild boars[J]. Agric Biol, 2016, 51(6):845-852.
[37]	ASAMBE A, SACKEY A K B, TEKDEK L B. Prevalence of African swine fever virus and classical swine fever virus antibodies in pigs in Benue State, Nigeria[J]. Trop Anim Health Prod, 2018, 50(3):689-692.
[38]	GALLARDO C, FERNÁNDEZ-PINERO J, PELAYO V, et al. Genetic variation among African swine fever genotype Ⅱ viruses, eastern and central Europe[J]. Emerg Infect Dis, 2014, 20(9):1544-1547.
[39]	NIETO-PELEGRÍN E, RIVERA-ARROYO B, SÁNCHEZ-VIZCAÍNO J M. First detection of antibodies against African swine fever virus in Faeces samples[J]. Transbound Emerg Dis, 2015, 62(6):594-602.
[40]	GIMÉNEZ-LIROLA L G, MUR L, RIVERA B, et al. Detection of African swine fever virus antibodies in serum and oral fluid specimens using a recombinant protein 30(p30) dual matrix indirect ELISA[J]. PLoS One, 2016, 11(9):e0161230.
[41]	张蕾, 董春娜, 李静, 等. 非洲猪瘟病毒间接ELISA抗体检测方法的建立[J]. 病毒学报, 2020, 36(4):670-674.ZHANG L, DONG C N, LI J, et al. Development of an ELISA based on synthetic peptides for detection of antibodies against the African swine fever virus[J]. Chinese Journal of Virology, 2020, 36(4):670-674. (in Chinese)
[42]	CARLSON J, ZANI L, SCHWAIGER T, et al. Simplifying sampling for African swine fever surveillance: Assessment of antibody and pathogen detection from blood swabs[J]. Transbound Emerg Dis, 2018, 65(1):e165-e172.
[43]	林彦星, 曹琛福, 张彩虹, 等. 非洲猪瘟病毒抗体量子点检测试纸条的研制[J]. 中国兽医科学, 2017, 47(10):1214-1220.LIN Y X, CAO C F, ZHANG C H, et al. Establishment of a quantum dots-based immunochromatographic strip for detection of the antibodies against African swine fever virus[J]. Chinese Veterinary Science, 2017, 47(10):1214-1220. (in Chinese)
[44]	MUR L, IGOLKIN A, VARENTSOVA A, et al. Detection of African swine fever antibodies in experimental and field samples from the Russian federation:Implications for control[J]. Transbound Emerg Dis, 2016, 63(5):e436-e440.
[45]	AIRA C, RUIZ T, DIXON L, et al. Bead-based multiplex assay for the simultaneous detection of antibodies to African swine fever virus and classical swine fever virus[J]. Front Vet Sci, 2019, 6:306.
[46]	MURGIA M V, MOGLER M, CERTOMA A, et al. Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus[J]. Arch Virol, 2019, 164(2):359-370.
[47]	GALLARDO C, BLANCO E, RODRÍGUEZ J M, et al. Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells[J]. J Clin Microbiol, 2006, 44(3):950-956.
[48]	AFAYOA M, OLAHO-MUKANI W, OKUNI J B, et al. Development and evaluation of an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) for the diagnosis of African swine fever[J]. Virol Mycol, 2015, 4(2):145.
[49]	SASTRE P, GALLARDO C, MONEDERO A, et al. Development of a novel lateral flow assay for detection of African swine fever in blood[J]. BMC Vet Res, 2016, 12:206.
[50]	吴海涛, 成大荣, 吴萌, 等. 非洲猪瘟病毒胶体金免疫层析试纸条的研制[J]. 黑龙江畜牧兽医, 2018(17):126-128, 238.WU H T, CHENG D R, WU M, et al. Preparation of colloidal gold immunochromatographic test strip of African swine fever virus[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(17):126-128, 238. (in Chinese)
[51]	SZEREDI L, BAKCSA E, ZÁDORI Z, et al. Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody[J]. J Virol Methods, 2020, 282:113886.
[52]	PETROVAN V, YUAN F F, LI Y H, et al. Development and characterization of monoclonal antibodies against p30 protein of African swine fever virus[J]. Virus Res, 2019, 269:197632.
[53]	WU P, LOWE A D, RODRÍGUEZ Y Y, et al. Antigenic regions of African swine fever virus phosphoprotein P30[J/OL]. Transboundary and Emerging Diseases, 2020, doi:10. 1111/tbed. 13533.
[54]	VLAD P. Antigenic characterization of African swine fever virus (ASFV) p30 and p54 proteins[D]. Manhattan: Kansas State University, 2019.
[55]	PETROVAN V, MURGIA M V, WU P, et al. Epitope mapping of African swine fever virus (ASFV) structural protein, p54[J]. Virus Res, 2020, 279:197871.
[56]	HEIMERMAN M E, MURGIA M V, WU P, et al. Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen[J]. J Vet Diagn Invest, 2018, 30(3):406-412.
[57]	QUEMBO C J, JORI F, VOSLOO W, et al. Genetic characterization of African swine fever virus isolates from soft ticks at the wildlife/domestic interface in Mozambique and identification of a novel genotype[J]. Transbound Emerg Dis, 2018, 65(2):420-431.
[58]	BLOME S, FRANZKE K, BEER M. African swine fever-A review of current knowledge[J]. Virus Res, 2020, 287:198099.
[59]	张洪亮, 金铭, 赵越, 等. 非洲猪瘟病毒免疫学及疫苗研究进展[J]. 病毒学报, 2019, 35(3):533-541.ZHANG H L, JIN M, ZHAO Y, et al. Research progress in the immunology and vaccine of the African swine fever virus[J]. Chinese Journal of Virology, 2019, 35(3):533-541. (in Chinese)
[60]	白晨雨, 王同燕, 赵少若, 等. 非洲猪瘟病毒p62蛋白单克隆抗体的制备及初步应用[J]. 畜牧兽医学报, 2020, 51(5): 1074-1082.BAI C Y, WANG T Y, ZHAO S R, et al. Preparation of monoclonal antibodies against recombinant p62 protein of African swine fever virus and its preliminary application[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1074-1082. (in Chinese)
[61]	ADENAIKE E A, TEKDEK L B, KAZEEM H M, et al. Antibody detection in suspected carriers of African swine fever virus in tarka local government area of Benue State, Nigeria[J]. Int J Prod Res, 2017, 29(2):289-293.
[62]	ANANDARAO R, SWAMINATHAN S, FERNANDO S, et al. Recombinant multiepitope protein for early detection of dengue infections[J]. Clin Vaccine Immunol, 2006, 13(1):59-67.
[63]	HE J, XIU B S, WANG G H, et al. Double-antigen sandwich ELISA for the detection of anti-hepatitis C virus antibodies[J]. J Virol Methods, 2011, 171(1):163-168.
[64]	SU Q D, GUO M Z, JIA Z Y, et al. Epitope-based recombinant diagnostic antigen to distinguish natural infection from vaccination with hepatitis A virus vaccines[J]. J Virol Methods, 2016, 233:41-45.
[65]	LAMPE K, GOTTSTEIN B, BECKER T, et al. Immunization of rhesus macaques with Echinococcus multilocularis recombinant 14-3-3 antigen leads to specific antibody response[J]. Parasitol Res, 2017, 116(1):435-439.
[66]	MIRZAPOUR A, SEYYED TABAEI S J, BANDEHPOUR M, et al. Designing a recombinant multi-epitope antigen of Echinococcus granulosus to diagnose human cystic echinococcosis[J]. Iran J Parasitol, 2020, 15(1):1-10.
[67]	MOHAN C M, DEY S, RAI A, et al. Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus[J]. J Virol Methods, 2006, 138(1-2):117-122.
[68]	ZHANG W B, WEN H, LI J, et al. Immunology and Immunodiagnosis of cystic echinococcosis: an update[J]. Clin Dev Immunol, 2012, 2012:101895.

Advances Research on African Swine Fever Serological Diagnostic Targets

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