肾复康颗粒对人工诱发鸡肾肿病理模型的炎性细胞因子及IL-1R/NF-κB信号通路的影响

doi:10.11843/j.issn.0366-6964.2020.11.027

Abstract

Abstract: The objective of the current study was to explore the anti-inflammatory mechanism of Shenfukang granule on chicken kidney swelling. Ninety healthy chickens were divided into blank group (n=15) and test group (n=75), the test group was replicated by the chicken kidney swelling model through feeding high-calcium and high-protein diet. After successful modeling, the test group was randomly divided into model group, positive drug group, Shenfukang high, medium and low dose group (n=15), Shenfukang granule 2.0, 1.0 and 0.5 g·L^-1, positive drug group, drinking water to Canglan oral liquid 1.0 mL·L^-1, The drinking water of the model group was given 1.0 mL·L^-1 of normal saline for 2 times·d^-1 and 5 days. Subpteral venous blood was collected before modeling, after modeling and 2 days after the last administration to isolate the serum. The content changes of serum IL-1, TNF-α, MCP-1 and TGF-β1 content were detected by ELISA. RT-PCR was used to detect renal IL-1R, IκBα, NF-κB mRNA transcription. Results were as follows: Compared with the blank group, the serum contents of IL-1, TNF-1, MCP-1 and TGF-β1 were significantly increased in the model group (P<0.05), the transcription of IL-1R, NF-κBp65 mRNA in kidney was significantly increased (P<0.01), and IκBα mRNA in kidney was significantly decreased (P<0.01). After drinking water treatment, the serum IL-1, TNF-α, MCP-1 and TGF-β1 contents of Shenfukang granule were significantly decreased in the high and low dose group and the positive drug group (P<0.01). The results of the current study suggested that Shenfukang granule played an anti-inflammatory role in inhibiting the IL-1R/NF-κB signaling pathway by reducing the contents of IL-1, TNF-α, MCP-1 and TGF-β1 in serum of kidney swelling chicken.

Key words: Shenfukang granule, renal swelling, anti-inflammatory, transcription, IL-1R/NF-κB signaling pathway

CLC Number:

S853.9

LUO Yichen, YANG Qingchang, LIN Chunfa, YE Ruiling, SHANG Yangfei, HU Yu, LIU Juan. The Effect of Shenfukang Granule on Content of Inflammatory Cytokines and the Signaling Pathway of IL-1R/NF-κB Induced by the Artificial Pathological Model of Chicken Kidney Swelling[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2886-2894.

References 35

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The Effect of Shenfukang Granule on Content of Inflammatory Cytokines and the Signaling Pathway of IL-1R/NF-κB Induced by the Artificial Pathological Model of Chicken Kidney Swelling

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