细粒棘球绦虫泛素结合酶基因家族的生物信息学及表达分析

doi:10.11843/j.issn.0366-6964.2023.06.037

Abstract

Abstract: To further explore the gene function and construct the dominant epitope vaccine, sequences identification, bioinformatics and transcription analysis of ubiquitin-conjugating enzyme gene family of Echinococcus granulosus sensu lato (EgE2s) were conducted. Based on the genomic data of Echinococcus granulosus published before, the EgE2s gene family was cloned and analyzed by bioinformatics. In addition, the transcriptional levels of EgE2s in protoscolex (PSCs) and 28-day strobilated worms were detected by qRT-PCR. The results showed that, 19 EgE2s genes were cloned and sequenced successfully. CDS sequences among two of them were originally wrong had been corrected and submitted to NCBI to obtain accession number (EgE2L3: MZ277618; EgE2J1: MZ277619). The prediction of tertiary structures and conservative motifs indicated that EgE2s were highly conserved. qRT-PCR showed that the transcriptional levels of EgE2s in PSCs differed from 28-day strobilated worms, EgE2A (P<0.001) and EgE2J1 (P<0.01) were highly expressed in PSCs. The prediction of B cell epitopes showed that the 139-157 region of EgE2N was located outside the conserved motifs of EgE2s. Meanwhile, the T cell epitopes prediction showed that EgE2N had two strong binding sites with human and canine MHC I molecules, respectively, including a common epitope region 104-113. In conclusion, EgE2A and EgE2J1 may play important roles in the growth and development of PSCs. The 139-157 and 104-113 regions of EgE2N may be the target sequences for the development of drugs and vaccines.

Key words: Echinococcus granulosus, ubiquitin-conjugating enzyme gene family, bioinformatics, transcription level analysis

CLC Number:

S852.734

DU Xiaodi, HOU Wei, SU Zhonghua, MA Qingmei, HE Xue, HUA Ruiqi, YANG Aiguo, YANG Guangyou. Bioinformatics and Expression Analysis of Ubiquitin-conjugating Enzyme Gene Family of Echinococcus granulosus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2605-2618.

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Bioinformatics and Expression Analysis of Ubiquitin-conjugating Enzyme Gene Family of Echinococcus granulosus

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