口蹄疫灭活疫苗的免疫效果及其对鉴别诊断的干扰

doi:10.11843/j.issn.0366-6964.2020.10.016

Abstract

Abstract: Vaccination with inactivated vaccine is an important measure to prevent and control foot-and-mouth disease (FMD), however, the immune effect and antigenic purity of inactivated vaccines are two major concerns for the establishment and evaluation of FMD free zones with vaccination. In this study, four groups of FMD type O and type A bivalent inactivated vaccines from 3 FMD vaccine manufacturers (designated as A, B and C) were selected to inoculate healthy juvenile cattle of FMD free. All cattle were immunized 3 or 4 times at a 1-month interval. Serum samples were collected before and after 1 month of every vaccination to determine the level of antibody to structural protein and non-structural protein. Results：(1) The qualified rates of antibody to structural protein: in group a1 (vaccine from company A, different batches), the antibody qualified rate could reach 100% for type O and type A, respectively after each vaccination. In group a2 (vaccines from company A, same batch), the antibody qualified rates were 36.7%, 98.3% and 100% for type O, and 15%, 86.7% and 100% for type A after the first to the third vaccination, respectively. In group b (vaccine from company B, same batch), the antibody qualified rates were 18.3%, 97% and 100% for type O, and 1.7%, 45% and 53.3% for type A after the first to the third vaccination, respectively. In group c (vaccines from company C, same batch), the antibody qualified rates were 26.7%, 96.7% and 100% for type O, and 21.7%, 71.7% and 100% for type A after the first to the third vaccination, respectively. (2) Antibody positive rate to non-structural protein 3ABC (confirmed with second ELISA test): In group a1, the positive rates were 0.7%, 1.4%, 9.5% and 4.8% after the first to the fourth vaccination, respectively; In group a2 and c, no 3ABC antibody-positive animal was detected after 3 repeated vaccination; In Group b, only one animal with a positive rate of 0.6% was detected after the third vaccination. The antibody qualified rates to the structural protein of FMDV in 3 of the 4 groups were far less than 70% after the primary vaccination, however, those were increased significantly after boost and repeated vaccination. The antigen purity of vaccines in three groups (a2, b and c) can meet the requirement of OIE standard on the FMD vaccine, however, the seroconversion to 3ABC antibody was obvious in animals from group a1 after repeated vaccination, which would cause some extent of interference to differential diagnosis. Also, a combination of a primary screening test and a confirmatory ELISA test can further improve the accuracy of differential diagnosis. This study provides an important scientific basis to make a rational program for establishment and evaluation of FMD free zone with vaccination.

Key words: foot-and-mouth disease, inactivated vaccine, immune efficacy, antigen purity, differential diagnosis

CLC Number:

S852.659.6

SUN Pu, HE Wei, FU Yuanfang, LI Dong, YANG Lin, WEI Delong, CAO Yimei, LI Pinghua, BAI Xingwen, MA Xueqing, LI Kun, BAO Huifang, ZHANG Jing, ZHU Xinrong, LIU Zaixin, LU Zengjun. Immune Effect of Inactivated Vaccines against Foot-and-Mouth Disease and Their Interference on Differential Diagnosis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2481-2489.

References 21

[1]	DE C BRONSVOORT B M, SØRENSEN K J, ANDERSON J, et al. Comparison of two 3ABC enzyme-linked immunosorbent assays for diagnosis of multiple-serotype foot-and-mouth disease in a cattle population in an area of endemicity[J]. J Clin Microbiol, 2004, 42(5):2108-2114.
[2]	KITCHING R P. Foot-and-mouth disease:current world situation[J]. Vaccine, 1999, 17(13-14):1772-1774.
[3]	LI D, BAI X W, SUN P, et al. Effect of the route of foot-and-mouth disease virus infection of piglets on the course of disease[J]. Acta Virol, 2010, 54(4):311-313.
[4]	刘在新. 全球口蹄疫防控技术及病原特性研究概观[J]. 中国农业科学, 2015, 48(17):3547-3564.LIU Z X. Progress and prospect of the technologies to control foot-and-mouth disease and its pathogen characteristics worldwide[J]. Scientia Agricultura Sinica, 2015, 48(17):3547-3564. (in Chinese)
[5]	中华人民共和国农业农村部. 中华人民共和国国务院办公厅关于印发《国家中长期动物疫病防治规划(2012-2020)的通知》[EB/OL]. 中华人民共和国农业部公报, 2012, 6:4-11. (2012-08-07)[2020-04-10]. http://jiuban.moa.gov.cn/zwllm/tzgg/gb/nybgg/201208/P020120807516465833069.pdf. The Ministry of Agriculture and Rural Affairs of the People's Republic of China. Circular of the General Office of the State Council of the People's Republic of China on Printing and Distributing the National Medium and Long-Term Programme for Animal Disease Control (2012-2020)[EB/OL]. Gazette of the Ministry of Agriculture of the People's Republic of China, 2012, 6:4-11. (2012-08-07)[2020-04-10]. http://jiuban.moa.gov.cn/zwllm/tzgg/gb/nybgg/201208/P02012-0807516465833069.pdf. (in Chinese)
[6]	中华人民共和国农业农村部. 农业部关于印发《国家口蹄疫防治计划(2016-2020年)》和《国家高致病性禽流感防治计划(2016-2020年)》的通知[EB/OL]. 中华人民共和国农业部公报, 2016, 9:53-56. (2012-08-07)[2020-04-10]. http://jiuban.moa.gov.cn/zwllm/tzgg/gb/nybgg/201611/P020161108589993604835.pdf. The Ministry of Agriculture and Rural Affairs of the People's Republic of China. Circular of the Ministry of Agriculture on printing and distributing the 2016-2020 National FMD Control Program and the 2016-2020 National HPAI Control Program[EB/OL]. Gazette of the Ministry of Agriculture of the People's Republic of China, 2016, 9:53-56. (2012-08-07)[2020-04-10]. http://jiuban.moa.gov.cn/zwllm/tzgg/gb/nybgg/201611/P020161108589993604835.pdf. (in Chinese)
[7]	FU Y F, LU Z J, LI P H, et al. Development of a blocking ELISA based on a monoclonal antibody against a predominant epitope in non-structural protein 3B2 of foot-and-mouth disease virus for differentiating infected from vaccinated animals[J]. PLoS One, 2014, 9(11):e111737.
[8]	FU Y F, LI P H, CAO Y M, et al. Development of a blocking ELISA using a monoclonal antibody to a dominant epitope in non-structural protein 3A of foot-and-mouth disease virus, as a matching test for a negative-marker vaccine[J]. PLoS One, 2017, 12(1):e0170560.
[9]	RADLETT P J, PAY T W, GARLAND A J. The use of BHK suspension cells for the commercial production of foot and mouth disease vaccines over a twenty year period[J]. Dev Biol Stand, 1985, 60:163-170.
[10]	谢庆阁. 口蹄疫[M]. 北京:中国农业出版社, 2004:1-15.XIE Q G. Foot-and-mouth disease[M]. Beijing:China Agriculture Press, 2004:1-15.
[11]	DE DIEGO M, BROCCHI E, MACKAY D, et al. The non-structural polyprotein 3ABC of foot-and-mouth disease virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle[J]. Arch Virol, 1997, 142(10):2021-2033.
[12]	CLAVIJO A, WRIGHT P, KITCHING P. Developments in diagnostic techniques for differentiating infection from vaccination in foot-and-mouth disease[J]. Vet J, 2004, 167(1):9-22.
[13]	LEE F, JONG M H, YANG D W. Presence of antibodies to non-structural proteins of foot-and-mouth disease virus in repeatedly vaccinated cattle[J]. Vet Microbiol, 2006, 115(1-3):14-20.
[14]	SMITSAART E, ESPINOZA A M, MARADEI E, et al. Importance of foot and mouth disease vaccine purity in interpreting serological surveys[J]. Rev Sci Tech, 2015, 34(3):755-766, 741-754.
[15]	Office International des Epizooties, OIE. Foot and mouth disease (infection with foot and mouth disease virus)[S]. OIE Terrestrial Manual, 2017.
[16]	中华人民共和国农业农村部. 农业部办公厅关于做好口蹄疫疫苗质量标准提升工作的通知[Z/OL]. (2013-04-06)[2020-04-10]. http://www.moa.gov.cn/govpublic/SYJ/201306/t20130613_3491208.htm. Ministry of Agriculture and Rural Affairs of the People's Republic of China. Notice of the General Office of the Ministry of Agriculture on improving the quality standard of foot-and-mouth Disease vaccine[Z/OL]. (2013-04-06)[2020-04-10]. http://www.moa.gov.cn/govpublic/SYJ/201306/t20130613_3491208.htm. (in Chinese)
[17]	中华人民共和国农业农村部. 中华人民共和国农业部公告第2078号[Z/OL]. (2014-03-12)[2020-04-10].http://jiuban.moa.gov.cn/zwllm/nybz/201403/t20140321_3825096.htm Ministry of Agriculture and Rural Affairs of the People's Republic of China. Announcement No. 2078 of Ministry of Agriculture of the People's Republic of China[Z/OL]. (2014-03-12)[2020-04-10].http://jiuban.moa.gov.cn/zwllm/nybz/201403/t20140321_3825096.htm (in Chinese)
[18]	魏孔福, 祁淑芸, 林密, 等. O型口蹄疫疫苗免疫牛抗体消长动态的LPB-ELISA检测[J]. 中国兽医科学, 2007, 37(9):787-790.WEI K F, QI S Y, LIN M, et al. Dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine by the liquid-phase blocking ELISA[J]. Veterinary Science in China, 2007, 37(9):787-790. (in Chinese)
[19]	赵丽霞, 关平原, 陈君彦, 等. 通过检测非结构蛋白抗体评价口蹄疫疫苗纯度的研究[J]. 中国兽药杂志, 2015, 49(4):17-20.ZHAO L X, GUAN P Y, CHEN J Y, et al. Study on the foot-and-mouth disease vaccine purity by testing for antibodies against nonstructural proteins[J]. Chinese Journal of Veterinary Drug, 2015, 49(4):17-20. (in Chinese)
[20]	王文莉, 赵俊皓, 刘华南, 等. 不同试剂盒检测接种FMDV的牛非结构蛋白抗体结果及分析[J]. 畜牧与兽医, 2018, 50(9):56-62.WANG W L, ZHAO J H, LIU H N, et al. Analysis of the results of non-structural protein antibody in cattle inoculated with FMDV using different kits[J]. Animal Husbandry & Veterinary Medicine, 2018, 50(9):56-62. (in Chinese)
[21]	BROCCHI E, BERGMANN I E, DEKKER A, et al. Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus[J]. Vaccine, 2006, 24(47-48):6966-6979.

Immune Effect of Inactivated Vaccines against Foot-and-Mouth Disease and Their Interference on Differential Diagnosis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 21

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHOU Guangqing, LIU Xiaoqing, SHI Xijuan, YANG Dapeng, YUAN Ligang, CHANG Huiyun. Establishment of a Rapid Detection SA-ELISA Method for Anti Foot-and-Mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2020-2029.
[2]	LI Shuo, ZHANG Yun, BAI Manyuan, ZHAO Ruichong, SONG Hetao, MU Suyu, TENG Zhidong, DONG Hu, MA E'ning, SUN Shiqi, GUO Huichen, YIN Shuanghui. Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1598-1607.
[3]	LI Rang, WENG Xiang, LI Quanxiao, WU Daocheng, CAO Hui, ZHANG Ailian. Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L. [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1608-1615.
[4]	GUO Zijing, CHEN Fei, ZHANG Zhixiong, BAI Ling, ZHANG Zhidong, LI Yanmin. Effects of Interleukin-10 on T Cell Proliferation and Its Expression of TNF-α, IFN-γ and IL-2 in Mice Infected with Foot-and-Mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 694-705.
[5]	MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.
[6]	HE Chenxiang, GAO Shandian, TIAN Zhancheng, DU Junzheng, WANG Jinming, GUAN Guiquan, YIN Hong. Development of a Differential Diagnostic ELISA Based on G_NS Protein to Distinguish BEFV Infected and Vaccinated Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4320-4326.
[7]	CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361.
[8]	HAO Jianwei, XUE Chunyi, CAO Yongchang. The Dose-titration Study of Inactivated Vaccine of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1536-1543.
[9]	HAN Weijian, ZHANG Junjuan, ZHANG Yiming, WANG Jiaxin, LI Limin. Cytokine Responses of Bone Marrow-derived Mast Cells to FMDV-VLPs via Mannose Receptors [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3917-3926.
[10]	YIMAMU·Ruziwanguli, ZHANG Ailian, WENG Xiang, XIAO Peng, CAO Hui, WU Daocheng. Comparison of Adjuvant Activity Between Xinjiang Cultivated/Wild Artemisia rupestris L. Crude Polysaccharides on Foot-and-mouth Disease Vaccine in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4089-4096.
[11]	WENG Xiang, ZHANG Ailian, LI Quanxiao, WU Daocheng, CAO Hui. Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 315-323.
[12]	ZHANG Zhidong, RYAN Eoin, LI Yanmin. Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2626-2632.
[13]	LI Xin, SUN Yanyan, LIN Mi, CHEN Xiahui, LI Fengsong, BAO Yanfang, YANG Guang, JIANG Tao. Establishment of Colloidal Gold Immunochromatographic Assay for Quantitative Detection of Foot-and-mouth Disease Virus Serotype O, A and Asia 1 [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1042-1052.
[14]	YAN Minghao, HAO Junhong, ZHANG Dajun, SHEN Chaochao, XU Guowei, HOU Jing, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. TPL2 Gene Knockout in PK-15 Cells Facilitates Replication of Foot-and-mouth Disease Virus and Seneca Valley Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1060-1073.
[15]	WANG Zhifang, ZHANG Wei, YANG Fan, WEI Ting, ZHENG Haixue. Gα12 Suppresses the Replication of Foot-and-mouth Disease Virus in PK-15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 556-564.