O、A、Asia 1型口蹄疫病毒胶体金定量检测免疫层析方法的建立

doi:10.11843/j.issn.0366-6964.2021.04.019

Abstract

Abstract: To establish a rapid genotyping and quantitative detection method for the foot-and-mouth disease virus (FMDV), the rabbit anti-FMDV and guinea pig anti-FMDV antibodies were used for labeling colloidal gold and NC membrane with strips by double antibody sandwich method. And three types chromatographic strips for detecting serotype O, A and Asia 1 were prepared. A quantitative standard curve was fitted by detecting the 146S of the calibrated reference materials. The quality verification of the immunochromatographic method was evaluated by specificity, sensitivity，reproducibility and correlation with sucrose density gradient. The quantitative detection method established in this study was distinctive, and there was no cross-reactivity among three serotypes of FMDV. This method had no cross reaction with Senecavirus A(SVA), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV). The limits of quantitative detection of the serotype O, A and Asia 1 viruses were 0.567, 0.693 and 0.219 μg·mL^-1 respectively. The assay also showed good linearity with a linear fitting coefficient of determination (R²) higher than 0.97. The correlation coefficient between the newly established method with 3 types lateral flow assay and sucrose density gradient was higher than 0.9. The variation coefficients of the three kinds of lateral flow assay are less than 10%.The results indicated that colloidal gold immunochromatographic quantitative assays for serotype O, A and Asia 1 FMDV could be used for identification of antigen, serotyping and quantitative detection of 146S.

Key words: foot-and-mouth disease virus, colloidal gold immunochromatographic assay, quantitative detection, 146S

CLC Number:

S854.43

LI Xin, SUN Yanyan, LIN Mi, CHEN Xiahui, LI Fengsong, BAO Yanfang, YANG Guang, JIANG Tao. Establishment of Colloidal Gold Immunochromatographic Assay for Quantitative Detection of Foot-and-mouth Disease Virus Serotype O, A and Asia 1[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1042-1052.

References 33

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Establishment of Colloidal Gold Immunochromatographic Assay for Quantitative Detection of Foot-and-mouth Disease Virus Serotype O, A and Asia 1

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