栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果

doi:10.11843/j.issn.0366-6964.2022.01.031

Abstract

Abstract: The purpose of this study was to evaluate the adjuvant effects of cultivated Artemisia rupestris L. crude polysaccharides (CARCP) on inactivated foot-and-mouth disease vaccine (FMDV). ICR mice were subcutaneously administrated with different doses of CARCP combined with FMDV. ELISA was used for the detection of FMDV-specific antibodies. T cell subsets in spleen and lymph nodes, and the expression of dendritic cell surface molecules as well as regulatory T cells (Tregs) in spleen were detected by flow cytometry. Mice were weighed every week, and observed the growth condition of mice after immunization. Compared with FMDV alone, higher levels of FMDV-specific IgG, IgG1 and IgG2a were induced by both medium-dose and high-dose of CARCP (P<0.01). Medium-dose of CARCP not only significantly increased the percentage of CD4, CD8, and CD44 T cells in the spleen and lymph nodes of mice (P<0.05), but also promoted the expression of CD86 and MHC-II in DC (P<0.05), whereas the CD4⁺CD25⁺Foxp3⁺ T cells were significantly down-regulated (P<0.05). There was no noticeable influence on the growth and body weight of immunized mice (P>0.05). The results suggested that CARCP as an adjuvant for FMDV could augment the specific humoral and cellular immune responses via inducing DC maturation, inhibiting Tregs-level, which provided a basis for the development of polysaccharide adjuvant of FMDV.

Key words: Artemisia rupestris L., crude polysaccharide, adjuvant, foot-and-mouth disease vaccine

CLC Number:

S852.42

WENG Xiang, ZHANG Ailian, LI Quanxiao, WU Daocheng, CAO Hui. Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 315-323.

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Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route

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