栽培一枝蒿粗多糖混合口蹄疫疫苗乳化方法及稳定性分析

doi:10.11843/j.issn.0366-6964.2023.04.024

Abstract

Abstract: The study aimed to explore the efficacy of the different emulsification methods and stability of foot-and-mouth disease (FMD) vaccine adjuvanted with cultivated Artemisia rupestris L. crude polysaccharides (CARCP). ICR mice were subcutaneously immunized with FMD-Ag (inactivated foot-and-mouth disease viral antigen) adjuvanted with ISA-206 or ISA-206+CARCP prepared by stirred emulsification and ultrasonic emulsification. FMD-specific antibodies and T cell subsets from spleen were detected. After FMD-Ag+ISA-206 and FMD-Ag+ISA-206+CARCP by stirred emulsification were placed at 4℃ and 25℃ for 30 and 180 d, respectively, ICR mice were immunized with these vaccine formulations and tested humoral and cellular immunity for the analysis of stability. Results were as follows:Compared with commercial FMD vaccine, the effects of different FMD vaccine formulations prepared by stirred emulsification and ultrasonic emulsification had no significant difference on FMD-specific IgG and CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells (P>0.05). After placing at 4℃ for 30 and 180 d, compared with commercial FMD vaccine, there was no significant difference in IgG levels and CD4⁺, CD8⁺, CD4⁺CD44⁺, CD8⁺CD44⁺T cells in FMD-Ag+ISA-206 and FMD-Ag+ISA-206+CARCP group (P>0.05). After placing at 25℃ for 30 and 180 d, compared with commercial FMD vaccine, the levels of antibodies and T-cell subsets in FMD-Ag+ISA-206 group were significantly reduced (P<0.05), while there was no significant difference between antibody levels and T-cell subsets in FMD-Ag+ISA-206+CARCP group (P>0.05). Stirred and ultrasonic emulsification have similar immune effects on FMD vaccine. Under different storage conditions, CARCP enhanced the stability of FMD vaccine. These data provided some experimental thoughts for CARCP polysaccharide adjuvant in the development of FMD vaccine adjuvant.

Key words: cultivated Artemisia rupestris L., crude polysaccharides, adjuvant, foot-and-mouth disease vaccine, stability

CLC Number:

S852.4

LI Rang, WENG Xiang, LI Quanxiao, WU Daocheng, CAO Hui, ZHANG Ailian. Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L.[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1608-1615.

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Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L.

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