口蹄疫病毒受体整联蛋白在绵羊不同发育阶段组织中mRNA转录水平的分析

doi:10.11843/j.issn.0366-6964.2021.09.026

Abstract

Abstract: Field strains of foot-and-mouth disease virus (FMDV) utilize integrin heterodimers αvβ1, αvβ3, αvβ6 and αvβ8 as receptors to gain entry to cells. Using novel real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assays, levels of mRNA of the integrin subunits αv, β3, β6 and β8 were quantified in tissue samples taken from ovine foetuses at 45, 75 and 110 days of gestation, neonatal lambs and adult ewes. The results showed that in the coronary band that the level of β6 mRNA of adult ewes and neonatal lambs was lower than that of foetuses at 75 and 110 days of gestation; In the heart, the level of β6 mRNA of adult ewes, neonatal lambs and foetuses at 110 days of gestation was higher than that of foetuses at 45 and 75 days of gestation; In the muscle, the level of β6 and β8 mRNA of foetuses and neonatal lambs was higher than that of foetuses at 45 and 75 days of gestation. In the tongue epithelium, the level of β3 mRNA in adult ewes was lower than that in other stages. In the soft palate and tonsil, there was little variation in the level of integrin mRNA between stages. In this study, the quantitative changes in integrin subunit mRNA distribution in sheep across different developmental stages have been assessed for the first time, which would provide a useful insight into the role of integrins in the pathogenesis of FMD in vivo.

Key words: foot-and-mouth disease virus, integrins, qRT-PCR, sheep

CLC Number:

S852.16

ZHANG Zhidong, RYAN Eoin, LI Yanmin. Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2626-2632.

References 20

[1]	ALEXANDERSEN S, ZHANG Z, DONALDSON A I, et al. The pathogenesis and diagnosis of foot-and-mouth disease[J]. J Comp Pathol, 2003, 129(1):1-36.
[2]	BELSHAM G J. Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family;aspects of virus protein synthesis, protein processing and structure[J]. Prog Biophys Mol Biol, 1993, 60(3):241-260.
[3]	RYAN E, ZHANG Z, BROOKS H W, et al. Foot-and-mouth disease virus crosses the placenta and causes death in fetal lambs[J]. J Comp Pathol, 2007, 136(4):256-265.
[4]	RYAN E, HORSINGTON J, BROWNLIE J, et al. Foot-and-mouth disease virus infection in fetal lambs:tissue tropism and cytokine response[J]. J Comp Pathol, 2008, 138(2-3):108-120.
[5]	RYAN E, HORSINGTON J, DURAND S, et al. Foot-and-mouth disease virus infection in young lambs:pathogenesis and tissue tropism[J]. Vet Microbiol, 2008, 127(3-4):258-274.
[6]	STENFELDT C, PACHECO J M, BORCA M V, et al. Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A[J]. Acta Vet Scand, 2014, 56(1):42.
[7]	DONALDSON A I, FERRIS N P, WELLS G A. Experimental foot-and-mouth disease in fattening pigs, sows and piglets in relation to outbreaks in the field[J]. Vet Rec, 1984, 115(20):509-512.
[8]	ZHANG Z D, WATERS R, LI Y M. Pathogenesis of non-epithelial foot-and-mouth disease in neonatal animals[J]. Vet Microbiol, 2021, 254:108961.
[9]	BERINSTEIN A, ROIVAINEN M, HOVI T, et al. Antibodies to the vitronectin receptor (integrin alpha V beta 3) inhibit binding and infection of foot-and-mouth disease virus to cultured cells[J]. J Virol, 1995, 69(4):2664-2666.
[10]	JACKSON T, SHARMA A, GHAZALEH R A, et al. Arginine-glycine-aspartic acid-specific binding by foot-and-mouth disease viruses to the purified integrin αvβ3 in vitro[J]. J Virol, 1997, 71(11):8357-8361.
[11]	JACKSON T, SHEPPARD D, DENYER M, et al. The epithelial integrin αvβ6 is a receptor for foot-and-mouth disease virus[J]. J Virol, 2000, 74(11):4949-4956.
[12]	JACKSON T, MOULD A P, SHEPPARD D, et al. Integrin αvβ1 is a receptor for foot-and-mouth disease virus[J]. J Virol, 2002, 76(3):935-941.
[13]	JACKSON T, CLARK S, BERRYMAN S, et al. Integrin alphavbeta8 functions as a receptor for foot-and-mouth disease virus:role of the beta-chain cytodomain in integrin-mediated infection[J]. J Virol, 2004, 78(9):4533-4540.
[14]	FJELLBIRKELAND L, CAMBIER S, BROADDU V C, et al. Integrin αvβ8-mediated activation of transforming growth factor-β inhibits human airway epithelial proliferation in intact bronchial tissue[J]. Am J Pathol, 2003, 163(2):533-542.
[15]	MONAGHAN P, GOLD S, SIMPSON J, et al. The αvβ6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle[J]. J Gen Virol, 2005, 86(Pt 10):2769-2780.
[16]	QUAN M, MURPHY C M, ZHANG Z, et al. Determinants of early foot-and-mouth disease virus dynamics in pigs[J]. J Comp Pathol, 2004, 131(4):294-307.
[17]	RYAN E D. The pathogenesis of foot-and-mouth disease in foetal and neonatal lambs[D]. London:Royal Veterinary College, 2007.
[18]	WANG G X, WANG Y H, SHANG Y J, et al. How foot-and-mouth disease virus receptor mediates foot-and-mouth disease virus infection[J]. Virol J, 2015, 12(1):9.
[19]	BROWN J K, MCALEESE S M, THORNTON E M, et al. Integrin-αvβ6, a putative receptor for foot-and-mouth disease virus, is constitutively expressed in ruminant airways[J]. J Histochem Cytochem, 2006, 54(7):807-816.
[20]	CLARK R A, ASHCROFT G S, SPENCER M J, et al. Re-epithelialization of normal human excisional wounds is associated with a switch from αvβ5 to αvβ6 integrins[J]. Br J Dermatol, 1996, 135(1):46-51.

Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[3]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[4]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[5]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[6]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[7]	LI Yuexin, LIU Aiju, MA Xiaofei, ZHENG Zhong, HU Boxin, ZHI Yunxia, TIAN Shujun. Effect of TGFβR1 on the Function of Sheep Granulosa Cells Mediated by TGF-β/Smad Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3335-3347.
[8]	MA Youji, CHEN Pengfei, MA Qing, WU Yi. Effects of Different Exercise Amounts on Rumen Microflora Diversity of Tan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3393-3405.
[9]	LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.
[10]	ZHANG Ying, JIN Hua, HAN Yang, SUI Dan, XIAO Xin, HAO Xiujing, LI Min. Analysis of Microbial Diversity in Nasal Cavity of Sheep with Respiratory Disease and Their Environment [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3455-3465.
[11]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.
[12]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[13]	ZHAO Donghao, YUAN Meng, MA Kaiteng, DUAN Zhuo, ZHU Yixin, TANG Fang, HAN Keguang, HUO Nairui. Chelating Role of Sheep Bone Collagen Peptide to Cadmium and Its Protection Role against Liver Injuries Induced by Cadmium in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2641-2652.
[14]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[15]	HAN Xiuyuan, ZHAO Liang, WANG Chuang, QI Meiyu, YAO Yuchang. Nicotinic Acid Enhances Low Temperature Preservation of Sheep Sperm by Reducing Oxidative Stress Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1979-1989.