Bta-miR-677靶向线粒体抗病毒信号蛋白影响Ⅰ型干扰素的生成

doi:10.11843/j.issn.0366-6964.2020.01.017

Abstract

Abstract: This study was conducted to investigate the molecular mechanism of bta-miR-677 regulating the expression of type Ⅰ interferon (IFN). Bta-miR-677 was transfected into MDBK cells to detect the transcription level of type Ⅰ IFN and interferon stimulating genes (ISGs). Then we predicted the target gene of bta-miR-677 by TargetScan, and the target gene of bta-miR-677 was verified by dual-luciferase report gene system, qRT-PCR and Western blot assay. The effect of the target gene on IFN transcription was verified by siRNA knockdown. The results showed that the transcription of IFN-α/β in bta-miR-677 group were 2-4 times higher than that of the control group (P<0.001), moreover, the transcription of six ISGs(IFI6, OAS1Y, OAS1Z, RSAD2, MX1 and MX2) were up-regulated by 2-16 times (P<0.01 or P<0.001). By contrast, the transcription of IFN-α/β in bta-miR-677 inhibitor group were down-regulated (P<0.01 or P<0.05), then the transcription of IFI6, OAS1Y, OAS1Z, RSAD2, MX1 and MX2 were down-regulated (P<0.05 or P<0.01). Subsequently, we demonstrated that bta-miR-677 could target the 3'-UTR of MAVS (mitochondria antiviral signaling protein) and the expression of MAVS is inhibited. Meanwhile, silencing of MAVS up-regulated the expression of IFN-α/β and the six ISGs. The results showed that bta-miR-677 up-regulated the transcription level of IFN-α/β by targeting MAVS, and then increased the transcription of ISGs. This study reveals that bta-miR-677 up-regulated type Ⅰ IFN by targeting MAVS to increase the expression of ISGs, which provided important data for the development of antiviral drugs based on bta-miR-677.

Key words: bta-miR-677, typeⅠ interferon, interferon stimulating genes, mitochondria antiviral signaling protein

CLC Number:

S852.659.5

LIAO Zheng, JI Xinqin, LI Jizong, XIAO Fang, LIU Maojun, MAO Li, LI Wenliang, SUN Min. Bta-miR-677 Up-regulated the Expression of Type Ⅰ Interferon by Targeting Mitochondria Antiviral Signaling Protein[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 150-158.

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Bta-miR-677 Up-regulated the Expression of Type Ⅰ Interferon by Targeting Mitochondria Antiviral Signaling Protein

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