牛呼吸道合胞体病毒G和F蛋白的生物学功能

doi:10.11843/j.issn.0366-6964.2024.04.013

Abstract

Abstract: Bovine respiratory syncytial virus (BRSV) is one of the viral causes of bovine respiratory diseases worldwide, mainly causing severe lower respiratory tract infections in calves under 1 year of age. BRSV encodes a total of 9 structural proteins, among which attachment protein (G protein) and fusion protein (F protein) are the main envelope glycoproteins on the surface of BRSV, which participate in the process of viral adsorption and fusion of host cells. G and F proteins contain a variety of identifying epitopes that stimulate the body′s production of neutralizing antibody responses and are often the focus of vaccine development for Bovine respiratory syncytial disease (BRSD). Exploring the mechanism of G and F proteins in BRSV infection is of great significance for the analysis of viral pathogenesis and vaccine development. This article aims to review the structural functions of BRSV G and F proteins and the development of related vaccines, in order to provide reference for the development of BRSV vaccine and the prevention and control of BRSD.

Key words: bovine respiratory syncytial virus (BRSV), bovine respiratory syncytial disease (BRSD), attachment proteins (G proteins), fusion protein (F protein), vaccine

CLC Number:

S852.659.5

GUO Xuelian, LI Yongqin, LI Ruiqian, LI Hao, JIN Shuangyuan, WANG Xueyan, DU Jiawei, XU Lihua. Biological Functions of Bovine Respiratory Syncytial Virus G and F Proteins[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1478-1487.

References

[1] İNCE Ö B, ?EVIK M, ÖZGÜR E G, et al. Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey[J]. Trop Anim Health Prod, 2021, 54(1):4.
[2] SUDARYATMA P E, MEKATA H, KUBO M, et al. Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria[J]. Vet Microbiol, 2019, 235:80-85.
[3] SANTOS-RIVERA M, WOOLUMS A R, THORESEN M, et al. Bovine respiratory syncytial virus (BRSV) infection detected in exhaled breath condensate of dairy calves by near-infrared aquaphotomics[J]. Molecules, 2022, 27(2):549.
[4] MAHMOUD A H A, SLATE J R, HONG S, et al. Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves[J]. J Anim Sci, 2020, 98(8):skaa252.
[5] JIA S, YAO X, YANG Y Q, et al. Isolation, identification, and phylogenetic analysis of subgroup Ⅲ strain of bovine respiratory syncytial virus contributed to outbreak of acute respiratory disease among cattle in Northeast China[J]. Virulence, 2021, 12(1):404-414.
[6] ZHANG J, WANG W, YANG M J, et al. Development of a one-step multiplex real-time PCR assay for the detection of viral pathogens associated with the bovine respiratory disease complex[J]. Front Vet Sci, 2022, 9:825257.
[7] CHANG Y M, YUE H, TANG C. Prevalence and molecular characteristics of bovine respiratory syncytial virus in beef cattle in China[J]. Animals, 2022, 12(24):3511.
[8] DÍAZ F E, GUERRA-MAUPOME M, MCDONALD P O, et al. A recombinant BCG vaccine is safe and immunogenic in neonatal calves and reduces the clinical disease caused by the respiratory syncytial virus[J]. Front Immunol, 2021, 12:664212.
[9] MAINA T W, GREGO E A, BRODERICK S, et al. Immunization with a mucosal, post-fusion F/G protein-based polyanhydride nanovaccine protects neonatal calves against BRSV infection[J]. Front Immunol, 2023, 14:1186184.
[10] DE JONG R, STOCKHOFE-ZURWIEDEN N, BONSING J, et al. ChAd155-RSV vaccine is immunogenic and efficacious against bovine RSV infection-induced disease in young calves[J]. Nat Commun, 2022, 13(1):6142.
[11] SACCO R E, MENA I, PALMER M V, et al. An intranasal recombinant NDV-BRSV F_opt vaccine is safe and reduces lesion severity in a colostrum-deprived calf model of RSV infection[J]. Sci Rep, 2022, 12(1):22552.
[12] FURZE J, WERTZ G, LERCH R, et al. Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus[J]. J Gen Virol, 1994, 75(Pt 2):363-370.
[13] JOBE F, SIMPSON J, HAWES P, et al. Respiratory syncytial virus sequesters NF-κB subunit p65 to cytoplasmic inclusion bodies to inhibit innate immune signaling[J]. J Virol, 2020, 94(22):e01380-20.
[14] SCHMIDT U, BEYER J, POLSTER U, et al. Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein g protects against challenge with wild-type BRSV[J]. J Virol, 2002, 76(23):12355-12359.
[15] PASTEY M K, SAMAL S K. Analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion[J]. J Gen Virol, 1997, 78(Pt 8):1885-1889.
[16] KARGER A, SCHMIDT U, BUCHHOLZ U J. Recombinant bovine respiratory syncytial virus with deletions of the G or SH genes:G and F proteins bind heparin[J]. J Gen Virol, 2001, 82(Pt 3):631-640.
[17] CUI G L, LIU H C, LI X G, et al. Preliminary functional and phylogeographic analyses of the 72 nucleotide duplication region in the emerging human respiratory syncytial virus ON1 strain attachment glycoprotein gene[J]. Biomed Pharmacother, 2020, 123:109800.
[18] TAPIA L I, SHAW C A, AIDEYAN L O, et al. Gene sequence variability of the three surface proteins of human respiratory syncytial virus (HRSV) in Texas[J]. PLoS One, 2014, 9(3):e90786.
[19] BUCHHOLZ U J, GRANZOW H, SCHULDT K, et al. Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (HRSV):effects on host range and evaluation as a live-attenuated HRSV vaccine[J]. J Virol, 2000, 74(3):1187-1199.
[20] STOPE M B, KARGER A, SCHMIDT U, et al. Chimeric bovine respiratory syncytial virus with attachment and fusion glycoproteins replaced by bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins[J]. J Virol, 2001, 75(19):9367-9377.
[21] CHANG Y M, TANG C, YUE H. Molecular epidemiological investigation of bovine respiratory syncytial virus in yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5):2030-2041. (in Chinese)
常益铭, 汤承, 岳华. 牦牛源牛呼吸道合胞体病毒的分子流行病学调查[J]. 畜牧兽医学报, 2023, 54(5):2030-2041.
[22] GARCÍA-BEATO R, MELERO J A. The C-terminal third of human respiratory syncytial virus attachment (G) protein is partially resistant to protease digestion and is glycosylated in a cell-type-specific manner[J]. J Gen Virol, 2000, 81(Pt 4):919-927.
[23] ESCRIBANO-ROMERO E, RAWLING J, GARCÍA-BARRENO B, et al. The soluble form of human respiratory syncytial virus attachment protein differs from the membrane-bound form in its oligomeric state but is still capable of binding to cell surface proteoglycans[J]. J Virol, 2004, 78(7):3524-3532.
[24] RUSSELL C J, HURWITZ J L. Sendai virus-vectored vaccines that express envelope glycoproteins of respiratory viruses[J]. Viruses, 2021, 13(6):1023.
[25] STINE L C, HOPPE D K, KELLING C L. Sequence conservation in the attachement glycoprotein and antigenic diversity among bovine respiratory syncytial virus isolates[J]. Vet Microbiol, 54(3/4):201-221.
[26] DORELEIJERS J F, LANGEDIJK J P M, HÅRD K, et al. Solution structure of the immunodominant region of protein g of bovine respiratory syncytial virus[J]. Biochemistry, 1996, 35(47):14684-14688.
[27] LEME R A, DALL AGNOL A M, BALBO L C, et al. Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus[J]. Vet Quart, 2020, 40(1):83-96.
[28] FURZE J M, ROBERTS S R, WERTZ G W, et al. Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity[J]. Virology, 1997, 231(1):48-58.
[29] LANGEDIJK J P M, DE GROOT B L, BERENDSEN H J C, et al. Structural homology of the central conserved region of the attachment protein g of respiratory syncytial virus with the fourth subdomain of 55-kDa tumor necrosis factor receptor[J]. Virology, 1998, 243(2):293-302.
[30] GORMAN J J, MCKIMM-BRESCHKIN J L, NORTON R S, et al. Antiviral activity and structural characteristics of the nonglycosylated central subdomain of human respiratory syncytial virus attachment (G) glycoprotein[J]. J Biol Chem, 2001, 276(42):38988-38994.
[31] HOTARD A L, LAIKHTER E, BROOKS K, et al. Functional analysis of the 60-nucleotide duplication in the respiratory syncytial virus Buenos Aires strain attachment glycoprotein[J]. J Virol, 2015, 89(16):8258-8266.
[32] DE WAAL L, YÜKSEL S, BRANDENBURG A H, et al. Identification of a common HLA-DP4-restricted T-cell epitope in the conserved region of the respiratory syncytial virus G protein[J]. J Virol, 2004, 78(4):1775-1781.
[33] VARGA S M, WISSINGER E L, BRACIALE T J. The attachment (G) glycoprotein of respiratory syncytial virus contains a single immunodominant epitope that elicits both Th1 and Th2 CD4⁺ T cell responses[J]. J Immunol, 2000, 165(11):6487-6495.
[34] LANGEDIJK J P, MELOEN R H, TAYLOR G, et al. Antigenic structure of the central conserved region of protein G of bovine respiratory syncytial virus[J]. J Virol, 1997, 71(5):4055-4061.
[35] YOLITZ J, SCHWING C, CHANG J L, et al. Signal peptide of HIV envelope protein impacts glycosylation and antigenicity of gp120[J]. Proc Natl Acad Sci U S A, 2018, 115(10):2443-2448.
[36] OOMENS A G P, WERTZ G W. trans-complementation allows recovery of human respiratory syncytial viruses that are infectious but deficient in cell-to-cell transmission[J]. J Virol, 2004, 78(17):9064-9072.
[37] SCHLENDER J, ZIMMER G, HERRLER G, et al. Respiratory syncytial virus (RSV) fusion protein subunit F2, not attachment protein G, determines the specificity of RSV infection[J]. J Virol, 2003, 77(8):4609-4616.
[38] LAWLOR H A, SCHICKLI J H, TANG R S. A single amino acid in the F₂ subunit of respiratory syncytial virus fusion protein alters growth and fusogenicity[J]. J Gen Virol, 2013, 94(Pt 12):2627-2635.
[39] LÓPEZ J A, PEÑAS C, GARCÍA-BARRENO B, et al. Location of a highly conserved neutralizing epitope in the F glycoprotein of human respiratory syncytial virus[J]. J Virol, 1990, 64(2):927-930.
[40] CALDER L J, GONZÁLEZ-REYES L, GARCÍA-BARRENO B, et al. Electron microscopy of the human respiratory syncytial virus fusion protein and complexes that it forms with monoclonal antibodies[J]. Virology, 2000, 271(1):122-131.
[41] COLLINS P L, HUANG Y T, WERTZ G W. Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus[J]. Proc Natl Acad Sci U S A, 1984, 81(24):7683-7687.
[42] SUN Z W, PAN Y B, JIANG S B, et al. Respiratory syncytial virus entry inhibitors targeting the F protein[J]. Viruses, 2013, 5(1):211-225.
[43] MAGRO M, ANDREU D, GÓMEZ-PUERTAS P, et al. Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein[J]. J Virol, 2010, 84(16):7970-7982.
[44] BLAIS N, GAGNÉ M, HAMURO Y, et al. Characterization of Pre-F-GCN4t, a modified human respiratory syncytial virus fusion protein stabilized in a noncleaved prefusion conformation[J]. J Virol, 2017, 91(13):e02437-16.
[45] OOMENS A G P, MEGAW A G, WERTZ G W. Infectivity of a human respiratory syncytial virus lacking the SH, G, and F proteins is efficiently mediated by the vesicular stomatitis virus G protein[J]. J Virol, 2003, 77(6):3785-3798.
[46] OOMENS A G P, BEVIS K P, WERTZ G W. The cytoplasmic tail of the human respiratory syncytial virus F protein plays critical roles in cellular localization of the F protein and infectious progeny production[J]. J Virol, 2006, 80(21):10465-10477.
[47] MESHRAM C D, BAVISKAR P S, OGNIBENE C M, et al. The respiratory syncytial virus phosphoprotein, matrix protein, and fusion protein carboxy-terminal domain drive efficient filamentous virus-like particle formation[J]. J Virol, 2016, 90(23):10612-10628.
[48] ZIMMER G, TROTZ I, HERRLER G. N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus[J]. J Virol, 2001, 75(10):4744-4751.
[49] LOPES B R P, DA SILVA G S, DE LIMA MENEZES G, et al. Serine proteases in neutrophil extracellular traps exhibit anti-respiratory syncytial virus activity[J]. Int Immunopharmacol, 2022, 106:108573.
[50] LEEMANS A, BOEREN M, VAN DER GUCHT W, et al. Removal of the N-glycosylation sequon at position N116 located in p27 of the respiratory syncytial virus fusion protein elicits enhanced antibody responses after DNA immunization[J]. Viruses, 2018, 10(8):426.
[51] MAS V, NAIR H, CAMPBELL H, et al. Antigenic and sequence variability of the human respiratory syncytial virus F glycoprotein compared to related viruses in a comprehensive dataset[J]. Vaccine, 2018, 36(45):6660-6673.
[52] CRUNKHORN S. Viral diseases:zeroing in on RSV vaccine design[J]. Nat Rev Drug Discov, 2014, 13(1):17.
[53] LEVELY M E, BANNOW C A, SMITH C W, et al. Immunodominant T-cell epitope on the F protein of respiratory syncytial virus recognized by human lymphocytes[J]. J Virol, 1991, 65(7):3789-3796.
[54] VAN BLEEK G M, POELEN M C, VAN DER MOST R, et al. Identification of immunodominant epitopes derived from the respiratory syncytial virus fusion protein that are recognized by human CD4 T cells[J]. J Virol, 2003, 77(2):980-988.
[55] KAPLAN B S, HOFSTETTER A R, MCGILL J L, et al. Identification of a DRB3*011:01-restricted CD4⁺ T cell response against bovine respiratory syncytial virus fusion protein[J]. Front Immunol, 2023, 14:1040075.
[56] TAYLOR G, RIJSEWIJK F A M, THOMAS L T, et al. Resistance to bovine respiratory syncytial virus (BRSV) induced in calves by a recombinant bovine herpesvirus-1 expressing the attachment glycoprotein of BRSV[J]. J Gen Virol, 1998, 79(Pt 7):1759-1767.
[57] MARTÍNEZ I, MELERO J A. Enhanced neutralization of human respiratory syncytial virus by mixtures of monoclonal antibodies to the attachment (G) glycoprotein[J]. J Gen Virol, 1998, 79(Pt 9):2215-2220.
[58] JONES B G, SEALY R E, SURMAN S L, et al. Sendai virus-based RSV vaccine protects against RSV challenge in an in vivo maternal antibody model[J]. Vaccine, 2014, 32(26):3264-3273.
[59] ZHAN X Y, SLOBOD K S, JONES B G, et al. Sendai virus recombinant vaccine expressing a secreted, unconstrained respiratory syncytial virus fusion protein protects against RSV in cotton rats[J]. Int Immunol, 2015, 27(5):229-236.
[60] GRIEVES J L, YIN Z W, GARCIA-SASTRE A, et al. A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats[J]. Vaccine, 2018, 36(26):3842-3852.
[61] OLMEDILLAS E, CANO O, MARTÍNEZ I, et al. Chimeric Pneumoviridae fusion proteins as immunogens to induce cross-neutralizing antibody responses[J]. EMBO Mol Med, 2018, 10(2):175-187.
[62] LI H, REN H, ZHANG Y, et al. Intranasal vaccination with a recombinant protein CTA1-DD-RBF protects mice against hRSV infection[J]. Sci Rep, 2021, 11(1):18641.
[63] REN H, LI H, CAO L, et al. Intranasal immunization with HRSV prefusion F protein and CpG adjuvant elicits robust protective effects in mice[J]. Vaccine, 2022, 40(47):6830-6838.
[64] LI H, REN H, CAO L, et al. Comparison of the efficacy and safety of different immunization routes induced by human respiratory syncytial virus F protein with CpG adjuvant in mice[J]. Biochem Biophys Res Commun, 2022, 618:54-60.
[65] LI H, REN H, ZHOU Y Z, et al. HRSV prefusion-F protein with Adju-Phos adjuvant induces long-lasting Th2-biased immunity in mice[J]. PLoS One, 2022, 17(1):e0262231.
[66] MCGILL J L, KELLY S M, KUMAR P, et al. Efficacy of mucosal polyanhydride nanovaccine against respiratory syncytial virus infection in the neonatal calf[J]. Sci Rep, 2018, 8(1):3021.
[67] MADHI S A, POLACK F P, PIEDRA P A, et al. Respiratory syncytial virus vaccination during pregnancy and effects in infants[J]. N Engl J Med, 2020, 383(5):426-439.
[68] YAZICI Z, OZAN E, TAMER C, et al. Circulation of indigenous bovine respiratory syncytial virus strains in turkish cattle:the first isolation and molecular characterization[J]. Animals, 2020, 10(9):1700.
[69] KUMAGAI A, KAWAUCHI K, ANDOH K, et al. Sequence and unique phylogeny of G genes of bovine respiratory syncytial viruses circulating in Japan[J]. J Vet Diagn Invest, 2021, 33(1):162-166.
[70] HOPPE I B A L, DE MEDEIROS A S R, ARNS C W, et al. Bovine respiratory syncytial virus seroprevalence and risk factors in non-vaccinated dairy cattle herds in Brazil[J]. BMC Vet Res, 2018, 14(1):208.
[71] KLEM T B, RIMSTAD E, STOKSTAD M. Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway[J]. BMC Vet Res, 2014, 10:15.

Biological Functions of Bovine Respiratory Syncytial Virus G and F Proteins

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