基于转录组测序筛选鼠伤寒沙门菌hfq基因缺失菌的差异表达基因

doi:10.11843/j.issn.0366-6964.2019.12.023

Abstract

Abstract: This study aimed to analyze the adaptation to environmental changes and bacterial secretion pathways in transcriptome sequencing of hfq gene deletion strains of Salmonella typhimurium and screen related genes. Real-time fluorescence quantitative PCR was used to verify the transcriptome sequencing results of hfq gene deletion strains of S. typhimurium, so as to conduct in-depth analysis on the bacterial chemotactic pathways, bicomponent pathways and secretion pathways of the hfq gene deleted strain of S. typhimurium, and screen the relevant important regulatory genes. Results were as follows:Four co-expressed genes, including yiaD, STM3138, STM3216 and malE, were screened out in the bacterial chemotaxis pathways. Seven co-expressed genes, including spaP, invA, prgH, invE, spaS, invG and ssaV, were screened from the bacterial secretion pathways. Sixteen co-expressed genes, including ybfM, htrA, pagO, STM3138, STm3031, ttrB, hilD, pstS, STM1530, hilA, pgtC, hydH, pocR, STM3216, ttrR and fljB, were screened in the bacterial two-component pathways. Differentially expressed genes were screened out from bacterial chemotactic pathways,two-component pathways and secretion pathways in hfg gene deleted S.typhimurium strain.And hfg could regulate these genes to affect related functions such as motility and virulence. The results laid a certain foundation for the prevention and cure of Salmonella and further study of sRNA and hfg in Salmonella.

Key words: Salmonella, hfq deleted strain, sRNA chaperone Hfq

CLC Number:

YANG Yang, YANG Qi, DONG Ranran, PAN Yong, LI Chen, LIU Lijuan, WEN Ming, ZHOU Bijun, WANG Kaigong. Screening of Differentially Expressed Genes of Salmonella typhimurium hfq Gene Deletion Bacteria Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2578-2584.

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Screening of Differentially Expressed Genes of Salmonella typhimurium hfq Gene Deletion Bacteria Based on Transcriptome Sequencing

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