植物乳杆菌X86对大鼠子代早期发育和肠道菌群的影响

doi:10.11843/j.issn.0366-6964.2025.10.049

摘要/Abstract

摘要：

旨在探讨在妊娠和哺乳期间，母鼠摄入植物乳杆菌X86(Lactiplantibacillus plantarumX86，L. plantarumX86)对子代早期发育及肠道菌群的影响。选取16只妊娠期SD大鼠，分为对照组(n=8)和L. plantarum X86组(n=8)。L. plantarum X86组大鼠从妊娠晚期至哺乳早期每日灌胃L. plantarum X86。通过多种指标评价该菌对子代生长发育、肠道屏障功能和肠道细菌群落结构的影响。结果表明，L. plantarum X86可提高子代7日龄和14日龄体重以及心脏和肝脏系数，但降低了白细胞计数和血红蛋白含量。此外，L. plantarum X86下调了子代肠道中IL-6基因mRNA的表达，并上调了Occludin基因的mRNA表达。16S rRNA菌群多样性分析显示，L. plantarum X86显著改变了子代肠道细菌群落结构，增加了乳杆菌属(Lactobacillus)和罗姆布茨菌属(Romboutsia)的丰度，而降低了埃希-志贺菌属(Escherichia-Shigella)、葡萄球菌属(Staphylococcus)及阿克曼菌属(Akkermansia)的丰度，同时KEGG代谢通路整体丰度降低。综上，在妊娠晚期和哺乳早期补充L. plantarum X86可促进大鼠子代的生长发育，并对其肠道屏障功能和细菌群落结构产生有益影响。

关键词: 植物乳杆菌X86, 妊娠和哺乳期, 大鼠子代, 生长发育, 肠道菌群

Abstract:

The aim of this study was to investigate the effects of maternal supplementation with Lactiplantibacillus plantarumX86 during pregnancy and lactation on early offspring development and gut microbiota. Sixteen gregnant SD rats were selected and divided into control group (n=8) and L. plantarumX86 group (n=8). Rats in the L. plantarumX86 group were given Lactiplantibacillus plantarumX86 gavage from late pregnancy until early lactation. The impact on offspring growth, intestinal barrier function, and gut bacterial community structure were evaluated by various indexes. Results showed that L. plantarumX86 supplementation increased body weight at 7 and 14 days of age, and elevated heart and liver coefficients of the offspring; However, it reduced the white blood cell counts and hemoglobin content. Additionally, L. plantarum X86 supplementation downregulated IL-6 mRNA expression and upregulated Occludin mRNA expression in the intestines of rat offspring. 16S rRNA sequencing analysis revealed that the maternal supplementation with L. plantarumX86 during late pregnancy and lactation significantly altered the gut microbiota in rat offspring, increasing the relative abundance of Lactobacillus and Romboutsia while reducing that of Escherichia-Shigella, Staphylococcus, and Akkermansia. A broad reduction in KEGG metabolic pathway abundance was also observed. In conclusion, consumption of L. plantarumX86 during late pregnant and early lactation promotes offspring growth and development, and exerts beneficial effects on the gut barrier function and bacterial microbiota composition.

Key words: Lactiplantibacillus plantarumX86, pregnancy and lactation period, rat offsprings, growth and development, gut microbiota

中图分类号:

S816.7

谢秀兰, 王建东, 晏仕英, 高海慧, 杨宇为, 赵建. 植物乳杆菌X86对大鼠子代早期发育和肠道菌群的影响[J]. 畜牧兽医学报, 2025, 56(10): 5315-5327.

XIE Xiulan, WANG Jiandong, YAN Shiying, GAO Haihui, YANG Yuwei, ZHAO Jian. Effects of Lactiplantibacillus plantarum X86 on the Early Development and Gut Microbiota of Offspring in Rats[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 5315-5327.

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基因Gene	引物序列 Primer sequence	扩增片段/bp Fragments size	参考基因 Reference gene
GAPDH	Forward: TCTCTGCTCCTCCCTGTTCT	107	NM_017008.4
	Reverse: CGATACGGCCAAATCCGTTC
IL-1β	Forward: AGCTTTCGACAGTGAGGAGA	138	NM_031512.2
	Reverse: TGTCGAGATGCT0GCTGTGAG
IL-6	Forward: ACTTCCAGCCAGTTGCCTTCTTG	110	NM_012589.2
	Reverse: TGGTCTGTTGTGGGTGGTATCCTC
ZO-1	Forward: GCCAGCTTTAAGCCTCCAGA	144	NM_001106266.1
	Reverse: TGGCTTCGCTTGAGGTTTCT
Occludin	Forward: GGGGCGCAGCAGGTCT	181	NM_031329.3
	Reverse: GTGCATCTCTCCGCCATACA
Claudin-1	Forward: TGCCCTACTTTCCTGCTCCTGTC	115	NM_031699.3
	Reverse: CTTCCTTCGCCGCTGTCACAC
Cldn3	Forward: GAAGTACGCACCCACCAAGA	88	NM_031700.2
	Reverse: GTCCTTACGGTCATAGGCGG

试剂Reagent	体积/μL Volume
5×FastPfu缓冲液5×FastPfu Buffer	4
2.5 mmol·L^-1 dNTPs	2
上游引物Forward Primer(5 μmol·L^-1)	0.8
下游引物Reverse Primer(5 μmol·L^-1)	0.8
TransStart FastPfu聚合酶 TransStart FastPfu Polymerase	0.4
牛血清蛋白BSA	0.2
模板DNA Template DNA	10 ng
双蒸水ddH₂O	up to 20

步骤 Step	温度/℃ Temperature	时间 Duration	循环数 Cycle
预变性Pre-denaturation	95	3 min	1
变性Denaturation	95	30 s	27
退火Annealing	55	30 s
延伸Extension	72	45 s
延伸Extension	72	10 min	1

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