基于16S rDNA分析饲喂唾液乳杆菌XP132对白羽肉种鸡肠道菌群的影响

doi:10.11843/j.issn.0366-6964.2024.09.033

摘要/Abstract

摘要：

旨在评估1株替抗专利菌株唾液乳杆菌XP132对健康白羽肉种鸡肠道菌群的影响。选取12只6月龄白羽肉种鸡, 随机平均分为试验组和对照组, 每组6只。试验组白羽肉种鸡每日饲喂活菌数为1×10⁹ CFU的唾液乳杆菌XP132发酵液, 对照组饲喂PBS, 连续饲喂4周后, 基于16S rDNA测序方法, 比较两组鸡肠道菌群发生的相对改变。结果表明, 在饲喂唾液乳杆菌XP132后, 通过α多样性分析物种丰富度, 试验组菌群丰富程度高于对照组。在菌群组成的属水平上, 饲喂唾液乳杆菌XP132的肉种鸡肠道菌群中, 螺旋体科NK4A136属、梭状芽胞杆菌属、副拟杆菌属、罗氏菌属等有益物种丰度增加; 在种水平上, 饲喂唾液乳杆菌XP132组的肉种鸡肠道菌群中乳杆菌、拟杆菌、鼠李糖乳杆菌类等有益物种丰度增加。结果提示, 本试验条件下, 饲喂唾液乳杆菌XP132改变了白羽肉种鸡肠道菌群的丰富度, 增加了有益菌菌群的物种丰度。

关键词: 肠道菌群, 唾液乳杆菌, 16S rDNA, 白羽肉种鸡

Abstract:

In this study, the effect of a patent-protected strain, Lactobacillus salivarius XP132, on the gut microbiota of healthy white feathered broiler breeders was evaluated using 16S rDNA sequencing. Twelve 6-month-old white-feathered broilor breeders were selected and randomly divided into control growp and experimeat group, with 6 broilers in each group. Lactobacillus salivarius XP132 fermentation broth with 1×10⁹ CFU was fed to broiler breeders in experiment group per day for 4 weeks, while broiler breeders in the control group were fed with PBS. Gut microbiota were determined by 16S rDNA sequencing. The results showed that after feeding Lactobacillus salivarius XP132 the species richness was higher in the experimental group than that in the control group using α diversity analysis. At the genus level of microbial composition, there was an increase in the abundance of beneficial species such as Laehnospiraceae NK4A136 spp., Clostridium spp., Paramycetes spp., and Rhodococcus spp. in the gut microbiota of the experimental group compared to the control group. While at the species level, there was a significant increase in the abundance of beneficial species such as Lactobacillus spp., Anaplasmatales spp., and Lactobacillus rhamnosus spp. in the gut microbiota of the experimental group. The results showed that feeding Lactobacillus salivarius XP132 changed the richness of intestinal microbiota and increased the species abundance of beneficial bacteria in white-feathered broilers.

Key words: gut microbiota, Lactobacillus salivarius, 16S rDNA, white-feathered broiler breeder

中图分类号:

S831.5

何塔娜, 胡馨匀, 米洁兰, 高立, 张艳萍, 祁小乐, 崔红玉, 杨桂连, 高玉龙. 基于16S rDNA分析饲喂唾液乳杆菌XP132对白羽肉种鸡肠道菌群的影响[J]. 畜牧兽医学报, 2024, 55(9): 4091-4099.

Tana HE, Xinyun HU, Jielan MI, Li GAO, Yanping ZHANG, Xiaole QI, Hongyu CUI, Guilian YANG, Yulong GAO. Effect of Feeding Lactobacillus salivarius XP132 on the Gut Microbiota of White-feathered Broiler Breeder based on 16S rDNA Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4091-4099.

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