镉可通过影响大鼠肠道菌群致大脑皮质氧化应激

doi:10.11843/j.issn.0366-6964.2025.07.045

摘要/Abstract

摘要：

旨在探究镉是否可通过影响大鼠肠道菌群致大脑皮质氧化应激，将20只21日龄雌性Wistar大鼠随机分成对照组(Con组)和镉组(Cd组)，预饲养1周。镉组大鼠每天每只按每千克体重10 mg的剂量灌胃氯化镉(CdCl₂)，对照组大鼠每天每只灌胃等量双蒸水。4周后取大鼠新鲜粪便，制成粪菌悬液，随后将大鼠解剖采集大脑皮质。另将20只21日龄雌性Wistar大鼠用混合抗生素灌胃1周构建伪无菌模型后，随机分成粪菌移植对照组(FMTCon组)和粪菌移植镉组(FMTCd组)，FMTCon组大鼠每天每只每千克体重灌胃10 mL上述对照组大鼠粪菌悬液，FMTCd组大鼠每天每只每千克体重灌胃10 mL上述镉组大鼠粪菌悬液，处理4周后，解剖采集大鼠大脑皮质。比色法检测丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量，总超氧化物歧化酶(T-SOD)和过氧化氢酶(CAT)活性及总抗氧化能力(T-AOC)；Western blot检测核因子-E2相关因子2(Nrf2)和NADH醌脱氢酶1(NQO-1)蛋白表达量。结果显示，与对照组相比，镉组大鼠大脑皮质MDA含量显著升高(P＜0.05)，GSH含量、CAT和T-SOD活性、T-AOC、Nrf2和NQO-1蛋白表达量显著降低(P＜0.05)；与FMTCon相比，FMTCd组大鼠大脑皮质MDA含量显著升高(P＜0.05)，GSH含量、CAT活性、Nrf2和NQO-1蛋白表达量显著降低(P＜0.05)，T-SOD活性和T-AOC降低，但无显著性差异(P＞0.05)。结果提示，镉可以通过影响大鼠肠道菌群引起大脑皮质氧化应激。

关键词: 镉, 肠道菌群, 大鼠, 大脑皮质, 氧化应激

Abstract:

To explore whether cadmium can induce oxidative stress in the cerebral cortices by affecting the intestinal flora of rats, twenty 21-day-old female Wistar rats were randomly divided into control group (Con group) and cadmium group (Cd group) and pre-fed for 1 week. The rats in the Cd group were given cadmium chloride (CdCl₂) at a dose of 10 mg per kg of body weight by intragastric administration every day, while the rats in the Con group were given double distilled water at the same dose by intragastric administration (i.g.) every day. After 4 weeks, the fresh feces of the rats were collected to make fecal bacteria suspension, and then the cerebral cortices of the rats were dissected. In addition, twenty 21-day-old female Wistar rats were given mixed antibiotics for 1 week to construct a pseudo-aseptic model, and were randomly divided into fecal microbiota transplantation control group (FMTCon group) and fecal microbiota transplantation cadmium group (FMTCd group). The rats in the FMTCon group were given the above-mentioned fecal suspension of the rats in the Con group at a dose of 10 mL per kg of body weight by i.g. every day, and the rats in the FMTCd group were given the above-mentioned fecal suspension of the rats in the Cd group at a dose of 10 mL per kg of body weight by i.g. every day. After 4 weeks of treatment, the cerebral cortices of rats were dissected and collected. The contents of malondialdehyde (MDA) and reduced glutathione (GSH), the activities of total superoxide dismutase (T-SOD) and catalase (CAT) and the total antioxidant capacity (T-AOC) were detected by colorimetry. The protein expressions of nuclear factor E2-related factor 2 (Nrf2) and NADH quinone dehydrogenase 1 (NQO-1) were detected by Western blot. The results showed that compared with the Con group, the contents of MDA were significantly increased (P < 0.05), the contents of GSH, the activities of CAT and T-SOD, and the protein expressions of T-AOC, Nrf2 and NQO-1 were significantly decreased (P < 0.05) in the cerebral cortices of Cd group rats. Compared with FMTCon, the contents of MDA were significantly increased (P < 0.05), the content of GSH, the activities of CAT, the protein expressions of Nrf2 and NQO-1 were significantly decreased (P < 0.05), and the activities of T-SOD and T-AOC were decreased, but there were no significant difference (P>0.05) in the cerebral cortices of FMTCd group rats. The results suggested that cadmium could induce oxidative stress in the cerebral cortices by affecting the intestinal flora of rats.

Key words: cadmium, intestinal flora, rat, cerebral cortex, oxidative stress

中图分类号:

S856.9

陆乐, 罗贤祖, 黄心昱, 邹辉, 顾建红, 刘学忠, 卞建春, 刘宗平, 袁燕. 镉可通过影响大鼠肠道菌群致大脑皮质氧化应激[J]. 畜牧兽医学报, 2025, 56(7): 3540-3547.

LU Le, LUO Xianzu, HUANG Xinyu, ZOU Hui, GU Jianhong, LIU Xuezhong, BIAN Jianchun, LIU Zongping, YUAN Yan. Cadmium Can Induce Oxidative Stress in the Cerebral Cortices by Affecting the Intestinal Flora of Rats[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3540-3547.

图/表 4

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