五味子多糖纳米硒的体外抗氧化和肠道菌群调节作用研究

doi:10.11843/j.issn.0366-6964.2024.07.041

摘要/Abstract

摘要：

旨在探究五味子多糖纳米硒(SCP-Selenium nanoparticles，SCP-SeNPs)的体外抗氧化活性和肠道菌群调节作用。本研究通过ABTS⁺、DPPH自由基、OH^-和O₂^-体外清除以及还原力检测试验系统评价了SCP-SeNPs的体外抗氧化活性；进一步通过16S rRNA高通量测序技术探究其对小鼠肠道菌群的影响。结果显示，SCP-SeNPs具有良好的体外抗氧化活性，且对OH^-和O₂^-的清除活性优于五味子多糖(Schisandra Chinensis polysaccharide，SCP)。16S rRNA检测结果显示，SCP-SeNPs组小鼠盲肠菌群的α多样性指数高于空白对照组(BC组)(P>0.05)；β多样性指数表明SCP-SeNPs组与BC组菌群结构存在明显差异。肠道菌群结构分析显示，拟杆菌门、厚壁菌门是小鼠肠道菌群的主要构成。菌群结构差异分析显示，属水平上，SCP-SeNPs组普雷沃氏菌属、Muribaculum、杜波西氏菌属、栖粪杆菌属和梭菌属_UCG_014等有益菌的相对丰度显著增加(P < 0.05)。PICRUSt 2功能预测分析显示，蛋白酶体、过氧化物酶体以及苯丙氨酸代谢和托烷、哌啶、吡啶生物碱的生物合成这4个通路在SCP-SeNPs组显著富集(P < 0.05)。综上，SCP-SeNPs具有一定的抗氧化能力，可能通过提高肠道有益菌的丰度和调节肠道微生物的物质代谢发挥肠道保护作用，提示SCP-SeNPs可以作为一种新型功能性微量元素添加剂。

关键词: 五味子多糖, 纳米硒, 抗氧化, 肠道菌群

Abstract:

The aim of this study was to explore the in vitro antioxidant property and intestinal microbiota modulation effect of Schisandra Chinensis polysaccharide-selenium nanoparticles (SCP-SeNPs). The in vitro antioxidant property of SCP-SeNPs was evaluated using ABTS⁺, DPPH radicals, OH^- and O₂^- scavenging assays, and reducing power assays. Furthermore, the impact of SCP-SeNPs on the intestinal microbiota of mice was investigated using 16S rRNA high-throughput sequencing technology. The results showed that, SCP-SeNPs exhibited significant in vitro antioxidant property, with better scavenging effects on OH^- and O₂^- radicals compared to SCP. The 16S rRNA analysis indicated that the α-diversity index of cecal microbiota in the SCP-SeNPs group was higher than that of the Blank control (BC) group (P>0.05). The β-diversity index suggested notable differences in microbial composition between the SCP-SeNPs and BC groups. Intestinal microbiota structure analysis revealed that Bacteroidota and Firmicutes were the major constituents of the mouse intestinal microbiota. Differential analysis of microbial composition indicated that at the genus level, beneficial bacteria such as Prevotellaceae, Muribaculum, Dubosiella, and Faecalibaculum and Clostridia_UCG_014 were significantly enriched in the SCP-SeNPs group (P < 0.05). PICRUSt 2 functional prediction analysis revealed that the pathways of Proteasome, Peroxisome, Phenylalanine metabolism, and biosynthesis of Tropane, piperidine, and pyridine alkaloids were significantly enriched in the SCP-SeNPs group (P < 0.05). In conclusion, SCP-SeNPs has certain antioxidant capacity and may have the potential to regulate intestinal microbiota composition and impact microbial metabolism through the enhancement of beneficial bacteria. These results suggest that SCP-SeNPs can serve as a novel functional trace element supplement.

Key words: Schisandra Chinensis polysaccharide, selenium nanoparticles, antioxidant, intestinal microbiota

中图分类号:

S816.7

杜红旭, 苏利娟, 何政科, 谭晓燕, 张旭, 马琪, 曹立亭, 陈红伟, 甘玲. 五味子多糖纳米硒的体外抗氧化和肠道菌群调节作用研究[J]. 畜牧兽医学报, 2024, 55(7): 3234-3245.

Hongxu DU, Lijuan SU, Zhengke HE, Xiaoyan TAN, Xu ZHANG, Qi MA, Liting CAO, Hongwei CHEN, Ling GAN. Study on the in vitro Antioxidant and Intestinal Flora Modulating Effects of Schisandra Chinensis Polysaccharides-selenium Nanoparticles[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3234-3245.

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项目 Item	BC组 BC group	SCP组 SCP group	SCP-SeNPs组 SCP-SeNPs group	P值 P-value
初重/g IBW	25.95±0.533	25.50±1.513	25.37±1.080	0.930
末重/g FBW	31.18±0.875	32.55±1.277	32.03±0.789	0.334
平均日增重/(g·d^-1) ADG	0.45±0.039	0.55±0.067	0.51±0.023	0.390

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