畜牧兽医学报 ›› 2026, Vol. 57 ›› Issue (1): 540-552.doi: 10.11843/j.issn.0366-6964.2026.01.047

• 临床兽医 • 上一篇    下一篇

三七茎叶提取物对脂多糖攻毒下仔猪生长性能、腹泻指数及肠道健康的影响

赵琳1,5(), 邬昆富1, 车思艳1,5, 卢宇2,3(), 王梓4, 吴苗苗1,5()   

  1. 1.湖南农业大学动物科学技术学院 畜禽产品品质调控湖南省重点实验室,长沙 410128
    2.江苏省农业科学院动物免疫工程研究所,南京 210014
    3.兽用生物制品(泰州)国泰技术创新中心,泰州 225300
    4.中南大学生命科学学院 分子生物学研究中心,长沙 410083
    5.岳麓山实验室,长沙 410128
  • 收稿日期:2024-12-18 出版日期:2026-01-23 发布日期:2026-01-26
  • 通讯作者: 卢宇,吴苗苗 E-mail:1290213245@qq.com;luyu@jaas.ac.cn;miaomiaowu0316@hunau.edu.cn
  • 作者简介:赵琳,硕士生,主要从事动物营养与饲料研究,E-mail:1290213245@qq.com
  • 基金资助:
    国家兽用生物制品工程技术研究中心开放课题(GTKF(23)002);国家自然科学基金(82470126);湖湘青年英才计划(2023RC3030);湖南省自然科学基金(2022JJ30787);云南省重大科技专项计划(202202AE090032)

Effects of Panax notoginseng Stem and Leaf Extract on Growth Performance, Diarrhea Index and Intestinal Health of LPS-Challenged Piglets

ZHAO Lin1,5(), WU Kunfu1, CHE Siyan1,5, LU Yu2,3(), WANG Zi4, WU Miaomiao1,5()   

  1. 1.Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry,College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China
    2.Institute of Animal Immunology Engineering,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
    3.Guotai Technology Innovation Center of Veterinary Biological Products (Taizhou),Taizhou 225300,China
    4.Center for Molecular Biology,School of Life Sciences,Central South University,Changsha 410083,China
    5.Yuelushan Laboratory,Changsha 410128,China
  • Received:2024-12-18 Online:2026-01-23 Published:2026-01-26
  • Contact: LU Yu, WU Miaomiao E-mail:1290213245@qq.com;luyu@jaas.ac.cn;miaomiaowu0316@hunau.edu.cn

摘要:

旨在探索三七(Panax notoginseng,PN)茎叶的皂苷提取方法,并研究其对脂多糖(lipopolysaccharide,LPS)攻毒下仔猪生长性能、腹泻指数及肠道健康的影响。利用超声波提取法,通过单因素试验,优化超声时间、液料比、乙醇体积分数等三七茎叶总皂苷的提取条件。试验选用24头初始体重为(7.13±0.74) kg的“杜×长×大”三元断奶仔猪,随机分为对照组(基础日粮)、LPS组(基础日粮)、DEX(地塞米松)+LPS组(基础日粮)和PN+LPS组(基础日粮+0.4%三七茎叶提取物),每组6头猪。在试验第28天采集仔猪血清、结肠组织及结肠食糜样品,检测血清生化及结肠炎症相关指标,分析结肠微生物群构成并观察结肠组织形态。结果表明:1)三七茎叶在料液比1∶25(g∶mL)、超声时间1.5 h和乙醇体积分数60%时,总皂苷提取率最高;2)与模型组相比,PN处理的仔猪采食量明显升高(P<0.05),腹泻指数有降低趋势(P=0.06);3)添加PN能有效改善LPS诱导结肠组织形态,预防黏膜损伤;4)PN预饲能降低LPS攻毒仔猪结肠中普氏菌属(Prevotella_9)的丰度(P<0.05);5)PN处理能在mRNA水平促进结肠IL-4表达(P<0.05),并降低TNF-α表达量(P<0.05)。综上所述,饲粮中添加0.4%三七茎叶提取物可以改善仔猪生长性能,缓解LPS造成的仔猪肠道形态受损,调节肠道微生物群组成并缓解肠道炎症,从而改善仔猪腹泻。

关键词: 三七茎叶提取物, 仔猪腹泻, 生长性能, 肠道形态, 微生物, 炎症

Abstract:

This study aimed to explore the extraction method of saponins from the stems and leaves of Panax notoginseng (PN) and investigate its effects on the growth performance, diarrhea index and intestinal health of piglets challenged with lipopolysaccharide (LPS). Single-factor experiments were conducted and ultrasonic extraction method was used to optimize the extraction conditions of total saponins from Panax notoginseng stems and leaves, such as ultrasonic time, liquid-to-solid ratio, and ethanol volume fraction. The experiment selected 24 “Duroc × Landrace × Large White” piglets with an initial body weight of (7.13±0.74) kg. The piglets were randomly divided into a control group (basic diet), an LPS group (basic diet), a DEX (dexamethasone) + LPS group (basic diet) and a PN + LPS group (basic diet + 0.4% Panax notoginseng stem and leaf extract), with six piglets in each group. On the 28th day of the experiment, serum, colon tissue and colon chyme samples were collected from piglets to test serum biochemical and colon inflammation-related indicators, the composition of colon microbiota were analyzed and colon tissue morphology were observed. The results showed as follows: 1) The extraction yield of total saponins was the highest at solid-liquid ratio of 1∶25(g∶mL), ultrasonic time of 1.5 h and ethanol volume fraction of 60%. 2) Compared with the LPS group, the feed intake of piglets in PN treatment was significantly increased (P<0.05), and the diarrhea index was decreased (P=0.06). 3) Supplementation of PN can effectively improve the morphology of colon tissue induced by LPS and prevent mucosal injury; 4) PN pre-feeding reduced the abundance of Prevotella_9 in the colon of piglets challenged with LPS (P<0.05). 5) PN treatment could promote the expression of IL-4 at mRNA level (P<0.05) and reduce the expression of TNF-α at mRNA level (P<0.05). In summary, add 0.4% PN in dietary can improve the growth performance of piglets, alleviate the damage of intestinal morphology caused by LPS, regulate intestinal microorganisms and alleviate intestinal inflammation, thereby improving the diarrhea of piglets.

Key words: stem and leaf extract of Panax notoginseng, diarrhea in piglets, growth performance, intestinal morphology, microorganisms, inflammation

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