食品级重组乳酸乳球菌强组成型分泌猪肠道益生的pEGF-p40

doi:10.11843/j.issn.0366-6964.2025.05.023

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (5): 2243-2258.doi: 10.11843/j.issn.0366-6964.2025.05.023

食品级重组乳酸乳球菌强组成型分泌猪肠道益生的pEGF-p40

何容肖(), 吴杨博(), 张淑霞, 赵锃珏, 黄娟, 潘伟雄, 任芷欣, 黄浩滨, 吴佳辉, 吴海阳, 沈世彦, 孙崇军, 张玲华*()

华南农业大学生命科学学院广东省农业生物发育与环境适应重点实验室, 广州 510642

收稿日期:2024-05-28 出版日期:2025-05-23 发布日期:2025-05-27
通讯作者: 张玲华 E-mail:1287912247@qq.com;wybhhxx@stu.scau.edu.cn;lhzhang@scau.edu.cn
作者简介:何容肖(1997-)，女，广东茂名人，博士生，主要从事微生物与免疫研究，E-mail：1287912247@qq.com
吴杨博(2001-)，男，湖北武汉人，硕士生，主要从事微生物与免疫研究，E-mail：wybhhxx@stu.scau.edu.cn
第一联系人：
何容肖和吴杨博为同等贡献作者
基金资助:
校企合作产学研项目(h2019364；h20220334)

Intestinal Beneficial pEGF-p40 Secreted by a Food-grade Recombinant Lactococcus lactis Strong Constitutive Expression System

HE Rongxiao(), WU Yangbo(), ZHANG Shuxia, ZHAO Zengjue, HUANG Juan, PAN Weixiong, REN Zhixin, HUANG Haobin, WU Jiahui, WU Haiyang, SHEN Shiyan, SUN Chongjun, ZHANG Linghua*()

Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

Received:2024-05-28 Online:2025-05-23 Published:2025-05-27
Contact: ZHANG Linghua E-mail:1287912247@qq.com;wybhhxx@stu.scau.edu.cn;lhzhang@scau.edu.cn

摘要/Abstract

摘要：

猪表皮生长因子(pEGF)与三叶因子协同给药已被证明能有效促进猪的肠道增殖与修复。本研究旨在构建食品级重组乳酸乳球菌强组成型表达系统并将其用于融合表达pEGF以及对猪肠道有益生作用的鼠李糖乳杆菌衍生蛋白p40，以克服目前乳酸乳球菌重组表达中组成型表达量低的现状以及避免抗生素使用的问题，并初步探索一种更强效的猪肠道益生制剂。首先，通过金黄色葡萄球菌核酸酶报告系统，以常用乳酸乳球菌组成型启动子P32为对照，从乳酸乳球菌NZ9000组成型高表达蛋白质的启动子以及前人研究发现的乳酸乳球菌强组成型启动子中，可视化地筛选出乳酸乳球菌NZ9000的伸长因子Tu的启动子(P1)活性最强。进一步地，将pNZ8148的氯霉素抗性基因替换为乳链菌肽抗性基因，成功构建了具有良好的乳链菌肽耐受性且完全失去氯霉素抗性的食品级重组乳酸乳球菌强组成型表达系统NZ9000/pP1NR。同时，本研究利用该系统成功表达pEGF和融合蛋白pEGF-p40。细胞增殖试验结果显示，含有pEGF-p40的菌株培养上清更有效地促进猪小肠上皮细胞IPEC-J2的增殖。通过qRT-PCR检测了刺激后IPEC-J2细胞的SGLT-1、GLUT-2、SUC、EGFR和GLP2R，结果显示含有pEGF-p40的培养上清调节相关基因的表达情况不同于仅含有pEGF的培养上清。本研究构建了食品级重组乳酸乳球菌强组成型表达系统，成功表达了对猪肠道具有益生作用的pEGF-p40，为其他益生物质的安全生产提供借鉴，为开发一种强效的猪肠道益生制剂做了先行探究。

关键词: 乳酸乳球菌, 组成型表达, pEGF, p40, 肠道健康

Abstract:

Co-administration of porcine epidermal growth factor (pEGF) and trefoil factor 3 has been shown to be effective in promoting intestinal proliferation and repair in pigs. Herein, this study was to construct a food-grade recombinant Lactococcus lactis strong constitutive expression system for the fusion expression of pEGF as well as a porcine gut beneficial protein p40, derived from Lactobacillus rhamnosus. As such, the researchers sought to overcome the current status quo of the low levels of L. lactis recombinant constitutive expression as well as to avoid the use of antibiotics, and to preliminarily develop a more potent porcine gut beneficial agent. Firstly, using the commonly used L. lactis constitutive promoter P32 as a control and the Staphylococcus aureus nuclease reporter system, this study visually screened out the promoter of the elongation factor Tu of L. lactis NZ9000 (P1) outperformed the other promoters of L. lactis NZ9000 constitutively highly expressed proteins and the previously found strong constitutive promoters of L. lactis. Furthermore, by replacing the chloramphenicol resistance gene of pNZ8148 with the nisin resistance gene, a food-grade recombinant L. lactis strong constitutive expression system named NZ9000/pP1NR was successfully constructed, which possessed good nisin resistance and completely lost the chloramphenicol resistance. Using the system, pEGF and the fusion protein pEGF-p40 were successfully expressed. Cell proliferation assays on IPEC-J2 cells showed that the culture supernatant containing pEGF-p40 was more effectively in promoting the proliferation. SGLT-1, GLUT-2, SUC, EGFR and GLP2R were detected in IPEC-J2 cells after stimulation by qRT-PCR, indicating that the culture supernatant containing pEGF-p40 regulated the expression of the relevant genes differently from that of the culture supernatant containing pEGF alone. In this study, a food-grade recombinant L. lactis strong constitutive expression system was constructed. By which, pEGF-p40 with a beneficial effect on porcine intestines, was successfully expressed. This study provides a reference for the safe production of other probiotic substances, and preliminarily explored a potent beneficial preparation for the porcine intestine.

Key words: Lactococcus lactis, constructive secretion, pEGF, p40, intestinal health

中图分类号:

何容肖, 吴杨博, 张淑霞, 赵锃珏, 黄娟, 潘伟雄, 任芷欣, 黄浩滨, 吴佳辉, 吴海阳, 沈世彦, 孙崇军, 张玲华. 食品级重组乳酸乳球菌强组成型分泌猪肠道益生的pEGF-p40[J]. 畜牧兽医学报, 2025, 56(5): 2243-2258.

HE Rongxiao, WU Yangbo, ZHANG Shuxia, ZHAO Zengjue, HUANG Juan, PAN Weixiong, REN Zhixin, HUANG Haobin, WU Jiahui, WU Haiyang, SHEN Shiyan, SUN Chongjun, ZHANG Linghua. Intestinal Beneficial pEGF-p40 Secreted by a Food-grade Recombinant Lactococcus lactis Strong Constitutive Expression System[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2243-2258.

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参考文献 41

1	XU S , WANG D , ZHANG P , et al. Oral administration of Lactococcus lactis-expressed recombinant porcine epidermal growth factor stimulates the development and promotes the health of small intestines in early-weaned piglets[J]. J Appl Microbiol, 2015, 119 (1): 225- 235. doi: 10.1111/jam.12833
2	ZENG F H , HARRIS R C . Epidermal growth factor, from gene organization to bedside[J]. Semin Cell Dev Biol, 2014, 28, 2- 11. doi: 10.1016/j.semcdb.2014.01.011
3	ZHANG P , HOLOWATYJ A N , ROY T , et al. An SH3PX1-dependent endocytosis-autophagy network restrains intestinal stem cell proliferation by counteracting EGFR-ERK signaling[J]. Dev Cell, 2019, 49 (4): 574- 589. doi: 10.1016/j.devcel.2019.03.029
4	SHAHRAJABIAN M H , SUN W L . Mechanism of action of collagen and epidermal growth factor: A review on theory and research methods[J]. Mini Rev Med Chem, 2024, 24 (4): 453- 477. doi: 10.2174/1389557523666230816090054
5	BEDFORD A , LI Z , LI M , et al. Epidermal growth factor-expressing Lactococcus lactis enhances growth performance of early-weaned pigs fed diets devoid of blood plasma[J]. J Anim Sci, 2012, 90 (suppl_4): 4- 6. doi: 10.2527/jas.53973
6	BEDFORD A , HUYNH E , FU M L , et al. Growth performance of early-weaned pigs is enhanced by feeding epidermal growth factor-expressing Lactococcus lactis fermentation product[J]. J Biotechnol, 2014, 173, 47- 52. doi: 10.1016/j.jbiotec.2014.01.012
7	WANG D Y , XU S Y , LIN Y , et al. Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned piglets[J]. BMC Vet Res, 2014, 10, 171. doi: 10.1186/s12917-014-0171-1
8	WANG S J , GUO C H , ZHOU L , et al. Effects of dietary supplementation with epidermal growth factor-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets[J]. Br J Nutr, 2016, 115 (9): 1509- 1520. doi: 10.1017/S0007114516000738
9	HUYNH E , LI J L . Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing[J]. Appl Microbiol Biotechnol, 2015, 99, 4667- 4677. doi: 10.1007/s00253-015-6542-0
10	YANG Y Q , LIN Z Y , LIN Q Y , et al. Pathological and therapeutic roles of bioactive peptide trefoil factor 3 in diverse diseases: recent progress and perspective[J]. Cell Death Dis, 2022, 13 (1): 62. doi: 10.1038/s41419-022-04504-6
11	KAUR H , ALI S A , SHORT S P , et al. Identification of a functional peptide of a probiotic bacterium-derived protein for the sustained effect on preventing colitis[J]. Gut Microbes, 2023, 15 (2): 2264456. doi: 10.1080/19490976.2023.2264456
12	GUO M J , ZHANG C Y , ZHANG C C , et al. Lacticaseibacillus rhamnosus reduces the pathogenicity of Escherichia coli in chickens[J]. Front Microbiol, 2021, 12, 664604. doi: 10.3389/fmicb.2021.664604
13	BÄUERL C , ABITAYEVA G , SOSA-CARRILLO S , et al. P40 and P75 are singular functional muramidases present in the Lactobacillus casei /paracasei/rhamnosus taxon[J]. Front Microbiol, 2019, 10, 1420. doi: 10.3389/fmicb.2019.01420
14	张攀, 许蒙蒙, 林燕, 等. 重组乳酸乳球菌表达外源产物在养猪生产中的潜在应用[J]. 动物营养学报, 2015, 27 (12): 3677- 3682.
	ZHANG P , XU M M , LIN Y , et al. Potential application of expression of exogenous products by recombinant Lactococcus lactis in pig production[J]. Chinese Journal of Animal Nutrition, 2015, 27 (12): 3677- 3682.
15	高莹, 李淼, 孙元, 等. 乳酸乳球菌表达系统的发展现状与前景展望[J]. 微生物学报, 2022, 62 (3): 895- 905.
	GAO Y , LI M , SUN Y , et al. Development and prospects of Lactococcus lactis expression system[J]. Acta Microbiologica Sinica, 2022, 62 (3): 895- 905.
16	王慧, 劳晓, 黄琳琳, 等. 乳酸乳球菌表达系统及其启动子的研究进展[J]. 食品科学, 2022, 43 (11): 330- 336.
	WANG H , LAO X , HUANG L L , et al. Progress in research on Lactococcus lactis expression systems and their promoter regulatory elements[J]. Food Science, 2022, 43 (11): 330- 336.
17	DUONG T , MILLER M J , BARRANGOU R , et al. Construction of vectors for inducible and constitutive gene expression in Lactobacillus[J]. Microb Biotechnol, 2011, 4 (3): 357- 367. doi: 10.1111/j.1751-7915.2010.00200.x
18	ZHU D L , LIU F L , XU H J , et al. Isolation of strong constitutive promoters from Lactococcus lactis subsp. lactis N8[J]. FEMS Microbiol Lett, 2015, 362 (16): fnv107. doi: 10.1093/femsle/fnv107
19	SILVA W M , SOUSA C S , OLIVEIRA L C , et al. Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label-free quantitative proteomics[J]. Microb Biotechnol, 2019, 12 (2): 265- 274. doi: 10.1111/1751-7915.13305
20	KLEIN E Y , VAN BOECKEL T P , MARTINEZ E M , et al. Global increase and geographic convergence in antibiotic consumption between 2000 and 2015[J]. Proc Natl Acad Sci U S A, 2018, 115 (15): E3463- E3470.
21	KUMAR S B , ARNIPALLI S R , ZIOUZENKOVA O . Antibiotics in food chain: The consequences for antibiotic resistance[J]. Antibiotics(Basel), 2020, 9 (10): 688.
22	郭婷婷, 孔文涛. 乳酸乳球菌nisin抗性基因的克隆及作为筛选标记的研究[J]. 山东大学学报(理学版), 2008, 43 (7): 78- 82.
	GUO T T , KONG W T . Cloning of a nisin resistance gene from Lactococcus lactis and its application in food-grade selection marker[J]. Journal of Shandong University(Natural Science), 2008, 43 (7): 78- 82.
23	梁琰, 崔欣, 王哲, 等. 乳酸菌食品级表达载体的研究与应用[J]. 微生物学通报, 2021, 48 (3): 906- 915.
	LIANG Y , CUI X , WANG Z , et al. Research and application of food-grade expression vectors of lactic acid bacteria[J]. Microbiology China, 2021, 48 (3): 906- 915.
24	IBRAHEIM H K , FAYEZ R A , JASIM A S , et al. Role of nuc gene in Staphylococcus aureus to phagocytic activity in different cattle infections[J]. Open Vet J, 2023, 13 (8): 1021- 1026. doi: 10.5455/OVJ.2023.v13.i8.8
25	FEITO J , ARAÚJO C , ARBULU S , et al. Design of Lactococcus lactis strains producing garvicin A and/or garvicin Q, either alone or together with nisin A or nisin Z and high antimicrobial activity against Lactococcus garvieae[J]. Foods, 2023, 12 (5): 1063. doi: 10.3390/foods12051063
26	ALIAS N A R , SONG A A L , ALITHEEN N B , et al. Optimization of signal peptide via site-directed mutagenesis for enhanced secretion of heterologous proteins in Lactococcus lactis[J]. Int J Mol Sci, 2022, 23 (17): 10044. doi: 10.3390/ijms231710044
27	GROTE A , HILLER K , SCHEER M , et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host[J]. Nucleic Acids Res, 2005, 33 (suppl_2): W526- W531.
28	HOOVER D . Using DNAWorks in designing oligonucleotides for PCR-based gene synthesis[J]. Methods Mol Biol, 2012, 852, 215- 223.
29	CLAES I J J , SCHOOFS G , REGULSKI K , et al. Genetic and biochemical characterization of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG[J]. PLoS One, 2012, 7 (2): e31588. doi: 10.1371/journal.pone.0031588
30	CHEN X Y , ZARO J L , SHEN W C . Fusion protein linkers: property, design and functionality[J]. Adv Drug Deliv Rev, 2013, 65 (10): 1357- 1369. doi: 10.1016/j.addr.2012.09.039
31	VILLATORO-HERNANDEZ J , LOERA-ARIAS M J , GAMEZ-ESCOBEDO A , et al. Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by Lactococcus lactis[J]. Microb Cell Fact, 2008, 7, 22. doi: 10.1186/1475-2859-7-22
32	JIANG S Y , LIU S Q , ZHAO C J , et al. Developing protocols of tricine-SDS-PAGE for separation of polypeptides in the mass range 1-30 kDa with minigel electrophoresis system[J]. Int J Electrochem Sci, 2016, 11 (1): 640- 649. doi: 10.1016/S1452-3981(23)15870-6
33	PAN W X , ZHAO Z J , WU J H , et al. LACpG10-HL functions effectively in antibiotic-free and healthy husbandry by improving the innate immunity[J]. Int J Mol Sci, 2022, 23 (19): 11466. doi: 10.3390/ijms231911466
34	MA M P , ZHAO Z T , LIANG Q Y , et al. Overexpression of pEGF improved the gut protective function of Clostridium butyricum partly through STAT3 signal pathway[J]. Appl Microbiol Biotechnol, 2021, 105 (14-15): 5973- 5991. doi: 10.1007/s00253-021-11472-y
35	余楠楠, 陈琛. Nisin抗菌肽在食品抗菌防腐中的应用[J]. 食品研究与开发, 2020, 41 (17): 198- 204.
	YU N N , CHEN C . Application of nisin antibacterial peptide in food preservation[J]. Food Research and Development, 2020, 41 (7): 198- 204.
36	SONG A A , IN L L A , LIM S H E , et al. A review on Lactococcus lactis: from food to factory[J]. Microb Cell Fact, 2017, 16, 55. doi: 10.1186/s12934-017-0669-x
37	DUARTE S O D , MONTEIRO G A . Plasmid replicons for the production of pharmaceutical-grade pDNA, proteins and antigens by Lactococcus lactis cell factories[J]. Int J Mol Sci, 2021, 22 (3): 1379. doi: 10.3390/ijms22031379
38	VAN TILBURG A Y , CAO H J , VAN DER MEULEN S B , et al. Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories[J]. Curr Opin Biotechnol, 2019, 59, 1- 7.
39	冯瑜菲, 胡清泉, 张力国, 等. 表达猪圆环病毒3型Cap蛋白重组乳酸乳球菌的构建及免疫原性分析[J]. 中国预防兽医学报, 2022, 44 (11): 1201- 1207.
	FENG Y F , HU Q Q , ZHANG L G , et al. Construction and immunogenicity evaluation of recombinant Lactococcus lactis expressing the Cap protein of porcine circovirus virus type 3[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44 (11): 1201- 1207.
40	BEDFORD A , CHEN T , HUYNH E , et al. Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved[J]. J Biotechnol, 2015, 196-197, 9- 19. doi: 10.1016/j.jbiotec.2015.01.007
41	DOMÍNGUEZ-DÍAZ C , AVILA-ARREZOLA K E , RODRÍGUEZ J A , et al. Recombinant p40 protein promotes expression of occludin in HaCaT keratinocytes: A Brief Communication[J]. Microorganisms, 2023, 11 (12): 2913. doi: 10.3390/microorganisms11122913

质粒名称 Plasmids	用途 Applications	来源 Sources
pDM19-T	T载体 T vector	购自宝日医生物技术(北京)有限公司 Purchased from Takara Biomedical Technology (Beijing) Co., Ltd.
pNZ8148	乳酸乳球菌表达载体 Expression vector for L. lactis	本实验室保存 Stored by the writer’s lab
pMG36e	乳酸乳球菌表达载体 Expression vector for L. lactis	本实验室保存 Stored by the writer’s lab
pDM19-T-SP-nuc	nuc基因的T载体 T vector containing nuc gene	本研究构建 Constucted by the current study
pNZ8148-SP-nuc	启动子PnisA控制下的nuc基因报告载体 Vecter with nuc reporter under control of PnisA
pNZ8148Δ(PnisA)-P1-SP-nuc	启动子P1控制下的nuc基因报告载体 Vecter with nuc reporter under control of P1
pNZ8148Δ(PnisA)-P2-SP-nuc	启动子P2控制下的nuc基因报告载体 Vecter with nuc reporter under control of P2
pNZ8148Δ(PnisA)-P3-SP-nuc	启动子P3控制下的nuc基因报告载体 Vecter with nuc reporter under control of P3
pNZ8148Δ(PnisA)-P4-SP-nuc	启动子P4控制下的nuc基因报告载体 Vecter with nuc reporter under control of P4
pNZ8148Δ(PnisA)-P5-SP-nuc	启动子P5控制下的nuc基因报告载体 Vecter with nuc reporter under control of P5
pNZ8148Δ(PnisA)-P8-SP-nuc	启动子P8控制下的nuc基因报告载体 Vecter with nuc reporter under control of P8
pNZ8148Δ(PnisA)-SP-nuc	启动子P32控制下的nuc基因报告载体 Vecter with nuc reporter under control of P32
pNZ8148Δ(PnisA)-P1	启动子P1控制下的表达载体 Expression vector under control of P1
pNZ8148Δ(PnisA)-P1-nsr	插入nsr基因的表达载体 Expression vector with nsr insertion
pP1NR	删除CmR基因的表达载体 Expression vector with CmR deletion
pP1NR-pEGF	pEGF的表达载体 Expression vector for pEGF
pP1NR-pEGF-p40	pEGF-p40的表达载体 Expression vector for pEGF-p40

基因 Gene	引物序列(5′→3′) Primer sequences	参考序列 References
β-actin	F: CACGCCATCCTGCGTCTGGA	XM_021086047.1
β-actin	R: AGCACCGTGTTGGCGTAGAG	XM_021086047.1
EGFR	F: GCCTTAGCCGTCTTATCCAA	NM_214007.1
EGFR	R: TGGGCACAGATGACTTTGGT	NM_214007.1
SGLT-1	F: CCACTTTCCCTATAAAACCTCAC	NM_001164021.1
SGLT-1	R: CTCCATCAAACTTCCATCCTCAG	NM_001164021.1
GLUT2	F: TTGCCTTGGATGAGTTATGTGA	NM_001097417.1
GLUT2	R: GCGTGGTCCTTGACTGAAAA	NM_001097417.1
SUC	F: TGGCCATCCAGTCATGCC	XM_047755123.1
SUC	R: CCACCACTCTGCTGTGGA	XM_047755123.1
GLP2R	F: CCCTGCTGTTTCTGGTTTCC	NM_001246266.1
GLP2R	R: GGCAGGGAACAGAAACGTTT	NM_001246266.1

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食品级重组乳酸乳球菌强组成型分泌猪肠道益生的pEGF-p40

Intestinal Beneficial pEGF-p40 Secreted by a Food-grade Recombinant Lactococcus lactis Strong Constitutive Expression System

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