植物活性成分对动物肠道屏障功能的调控作用及其机制

doi:10.11843/j.issn.0366-6964.2024.04.012

摘要/Abstract

摘要： 肠道不仅是动物机体消化、吸收营养物质的主要场所，同时也是机体发挥防御保护作用的重要组成部分之一，完整的肠道屏障可有效阻止抗原、细菌毒素和有害物质通过，对维持动物健康极为重要。近年来研究发现，天然植物活性成分(NPBCs)具有免疫调节、抑菌、抗氧化、改善肠道健康等多种生物学功能，在畜禽生产及疾病防治中有广泛的应用价值。本文就NPBCs对动物肠黏膜形态结构、肠道屏障功能以及肠道菌群结构的影响进行综述，旨在为深入了解NPBCs对动物肠道健康的调控作用及其在动物生产中的应用开发提供参考。

关键词: 天然植物活性成分, 肠道健康, 屏障功能

Abstract: Intestinal tract is not only the largest and most complex organ in the animal body, but also the component of body′s defense forefront. The integrity of the intestinal barrier is extremely important for maintaining animal health, as it can effectively prevent the passage of antigens, bacterial toxins and harmful macromolecules. In recent years, it has been found that natural plant bioactive compounds(NPBCs)have many biological functions such as immune regulation, bacteriostasis, antioxidant, improving intestinal health, etc., and have wide application value in livestock production and disease prevention and control. In this paper, the effects of NPBCs on intestinal morphology, intestinal barrier function and intestinal flora structure of animals were reviewed, aiming to provide references for further understanding of the regulatory effects of NPBCs on intestinal health and their application in animal production.

Key words: natural plant bioactive compounds, intestinal health, barrier function

中图分类号:

S816.7

牛晓雨, 邢媛媛, 李大彪. 植物活性成分对动物肠道屏障功能的调控作用及其机制[J]. 畜牧兽医学报, 2024, 55(4): 1467-1477.

NIU Xiaoyu, XING Yuanyuan, LI Dabiao. Advances in Regulation and Mechanism of Plant Bioactive Compounds on Intestinal Barrier Function in Animals[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1467-1477.

参考文献

[1] ANDERSON R C, DALZIEL J E, GOPAL P K, et al. The role of intestinal barrier function in early life in the development of colitis[M]//FUKATA M. Colitis. Rijeka:IntechOpen, 2012:26-54.
[2] CAMILLERI M. Leaky gut:mechanisms, measurement and clinical implications in humans[J]. Gut, 2019, 68(8):1516-1526.
[3] ZHU C, WU Y P, JIANG Z Y, et al. Dietary soy isoflavone attenuated growth performance and intestinal barrier functions in weaned piglets challenged with lipopolysaccharide[J]. Int Immunopharmacol, 2015, 28(1):288-294.
[4] SU D, WEN X B, MA T, et al. Effects of hydroxytyrosol on growth performance, antioxidant capacity and intestinal inflammatory factors in broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11):4851-4859. (in Chinese)
苏丹, 文晓宾, 马腾, 等. 羟基酪醇对肉仔鸡生长性能、抗氧化能力和肠道炎性因子的影响[J]. 畜牧兽医学报, 2023, 54(11):4851-4859.
[5] VEREDIANO T A, MARTINO H S D, PAES M C D, et al. Effects of anthocyanin on intestinal health:a systematic review[J]. Nutrients, 2021, 13(4):1331.
[6] GU X H, ZHANG H F, SHE R P, et al. Effect of weaning age on small intestine morphology in piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2001, 32(4):306-313. (in Chinese)
顾宪红, 张宏福, 佘锐萍, 等. 断奶日龄对仔猪肠粘膜形态的影响[J]. 畜牧兽医学报, 2001, 32(4):306-313.
[7] YUAN D X, WANG J, XIAO D F, et al. Eucommia ulmoides flavones as potential alternatives to antibiotic growth promoters in a low-protein diet improve growth performance and intestinal health in weaning piglets[J]. Animals, 2020, 10(11):1998.
[8] CHEN J S, KANG B J, ZENG J G, et al. Effects of dietary sanguinarine on growth performance, intestinal mucosal morphology and immune function of small intestinal mucosa of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2018, 30(5):1845-1853. (in Chinese)
陈家顺, 康宝聚, 曾建国, 等. 血根碱对断奶仔猪生长性能、肠道形态结构及小肠黏膜免疫功能的影响[J]. 动物营养学报, 2018, 30(5):1845-1853.
[9] CSERNUS B, BIRÓ S, BABINSZKY L, et al. Effect of carotenoids, oligosaccharides and anthocyanins on growth performance, immunological parameters and intestinal morphology in broiler chickens challenged with Escherichia coli lipopolysaccharide[J]. Animals, 2020, 10(2):347.
[10] JIANG S Q, CHEN Z L, ZHANG S, et al. Protective effects of protocatechuic acid on growth performance, intestinal barrier and antioxidant capacity in broilers challenged with lipopolysaccharide[J]. Animal, 2023, 17(1):100693.
[11] WU Y J, JIANG H H, ZHU E P, et al. Hericium erinaceus polysaccharide facilitates restoration of injured intestinal mucosal immunity in Muscovy duck reovirus-infected Muscovy ducklings[J]. Int J Biol Macromol, 2018, 107:1151-1161.
[12] LI C Y, NIU J L, LIU Y X, et al. The effects of oregano essential oil on production performance and intestinal barrier function in growing Hyla rabbits[J]. Ital J Anim Sci, 2021, 20(1):2165-2173.
[13] ZHENG C, LI F D, LI F, et al. Effects of tannic acid addition in milk replacer on development of gastrointestinal tract of 7 to 28 days old Hu lambs[J]. Scientia Agricultura Sinica, 2019, 52(21):3924-3933. (in Chinese)
郑琛, 李发弟, 李飞, 等. 代乳粉添加单宁酸对7-28日龄湖羊羔羊胃肠道发育的影响[J]. 中国农业科学, 2019, 52(21):3924-3933.
[14] LI Y, MENG X Y, LING Y, et al. Effects of tea saponin on intestinal mucosa morphology and intestinal flora of Anhui white goat[J]. Journal of Yangzhou University:Agricultural and Life Science Edition, 2021, 42(5):25-32. (in Chinese)
李玉, 孟新月, 凌嫄, 等. 茶皂素对安徽白山羊小肠黏膜形态及肠道菌群的影响[J]. 扬州大学学报(农业与生命科学版), 2021, 42(5):25-32.
[15] ZHUANG S, MING K, MA N, et al. Portulaca oleracea L. polysaccharide ameliorates lipopolysaccharide-induced inflammatory responses and barrier dysfunction in porcine intestinal epithelial monolayers[J]. J Funct Foods, 2022, 91:104997.
[16] XU C, WANG K, DING Y H, et al. Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice[J]. World J Gastroentero, 2019, 25(5):584-599.
[17] WAN S Z, LIU C, HUANG C K, et al. Ursolic acid improves intestinal damage and bacterial dysbiosis in liver fibrosis mice[J]. Front Pharmacol, 2019, 10:1321.
[18] RAKA R N, XIAO J S, WU H, et al. Pingyin rose essential oil restores intestinal barrier integrity in DSS-induced mice colitis model[J]. Food Res Int, 2023, 164:112362.
[19] WANG Y, SUN W J, WU E Y, et al. Polysaccharides from Abrus cantoniensis hance modulate intestinal microflora and improve intestinal mucosal barrier and liver oxidative damage induced by heat stress[J]. Front Vet Sci, 2022, 9:868433.
[20] BRUNING E E, COLLER J K, WARDILL H R, et al. Site-specific contribution of Toll-like receptor 4 to intestinal homeostasis and inflammatory disease[J]. J Cell Physiol, 2021, 236(2):877-888.
[21] TANG X P, LIU H, YANG S F, et al. Epidermal growth factor and intestinal barrier function[J]. Mediat Inflamm, 2016, 2016:1927348.
[22] REISINGER N, STEINER T, NITSCH S, et al. Effects of a blend of essential oils on broiler performance and intestinal morphology during coccidial vaccine exposure[J]. J Appl Poultry Res, 2011, 20(3):272-283.
[23] MEI C, HE S S, YIN P, et al. Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury[J]. J Zhejiang Univ-SCI B, 2016, 17(6):413-424.
[24] REN Z H, GUO C Y, YU S M, et al. Progress in mycotoxins affecting intestinal mucosal barrier function[J]. Int J Mol Sci, 2019, 20(11):2777.
[25] MEI H D, LI Y F, MA X Y, et al. Effects and regulatory mechanisms of quercetin on animal intestinal mucosal barrier function[J]. Chinese Journal of Animal Nutrition, 2022, 34(9):5475-5488. (in Chinese)
梅华迪, 李袁飞, 马现永, 等. 槲皮素对动物肠道黏膜屏障功能的影响及其调控机制[J]. 动物营养学报, 2022, 34(9):5475-5488.
[26] XIONG X J, CHEN W Y, CUI J L, et al. Effecte of Uvsolic acid on promoting bile secretion in rat[J]. Acta Chinese Medicine and Pharmacology, 2003, 31(4):54-55. (in Chinese)
熊筱娟, 陈蔚云, 崔江龙, 等. 乌索酸对大鼠胆汁的影响[J]. 中医药学报, 2003, 31(4):54-55.
[27] VOLSTATOVA T, MARCHICA A, HRONCOVA Z, et al. Effects of chlorogenic acid, epicatechin gallate, and quercetin on mucin expression and secretion in the Caco-2/HT29-MTX cell model[J]. Food Sci Nutr, 2019, 7(2):492-498.
[28] PI Y. Effects of different interventions on intestinal microbiota, host metabolism and gut barrier function in growing pigs[D]. Nanjing:Nanjing Agricultural University, 2019. (in Chinese)
皮宇. 不同方式干预对生长猪肠道微生物、机体代谢和肠道屏障功能的影响[D]. 南京:南京农业大学, 2019.
[29] LIU H W, LI K, ZHAO J S, et al. Effects of chestnut tannins on intestinal morphology, barrier function, pro-inflammatory cytokine expression, microflora and antioxidant capacity in heat-stressed broilers[J]. J Anim Physiol Anim Nutr, 2018, 102(3):717-726.
[30] SUN Y G, ZHANG X J, CHENG J, et al. Effects of two Chinese medicinal ingredients on growth performance, digestive Utilization ratios of nutrients, digestive enzyme activity and intestinal bacterial in broilers[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(4):93-98. (in Chinese)
孙耀贵, 张向杰, 程佳, 等. 2种中药成分对肉鸡生产性能、营养物质消化利用率、肠道消化酶活性和肠道菌群的影响[J]. 中国畜牧兽医, 2013, 40(4):93-98.
[31] LI H L. Study on physiological and biochemical characteristics and immune function of lactic acid bacteria and tea polyphenols on broilers[D]. Changsha:Hunan Agricultural University, 2015. (in Chinese)
李华丽. 乳酸菌与茶多酚对肉鸡生理生化性能及免疫机能的研究[D]. 长沙:湖南农业大学, 2015.
[32] CHENG Y, LIU Y R, CHEN D, et al. Dual effects of quercetin on protein digestion and absorption in the digestive tract[J]. Food Chem, 2021, 358:129891.
[33] TONG Z K, LEI F H, LIU L X, et al. Effects of Plotytarya strohilacea sieb. et zuce tannin on the growth performance, oxidation resistance, intestinal morphology and cecal microbial composition of broilers[J]. Front Vet Sci, 2022, 8:806105.
[34] DONG S, LI H, GASCO L, et al. Antioxidative activity of the polyphenols from the involucres of Castanea mollissima Blume and their mitigating effects on heat stress[J]. Poultry Sci, 2015, 94(5):1096-1104.
[35] XIONG Y, DONG S, ZHAO X, et al. Gene expressions and metabolomic research on the effects of polyphenols from the involucres of Castanea mollissima Blume on heat-stressed broilers chicks[J]. Poultry Sci, 2016, 95(8):1869-1880.
[36] LIU H J, WANG C, ZHOU B B, et al. Effects of dietary rutin supplementation on ileal morphology, immunity, antioxidant and barrier function of broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2):630-641. (in Chinese)
刘慧娟, 王超, 周斌斌, 等. 饲粮中添加芦丁对肉鸡回肠形态、免疫、抗氧化及屏障功能的影响[J]. 畜牧兽医学报, 2023, 54(2):630-641.
[37] WANG J. Screening of key proteins related to hepatic metabolism of weaned piglets after alfalfa saponins treatment[D]. Zhengzhou:Henan Agricultural University, 2015. (in Chinese)
王洁. 苜蓿皂苷调控断奶仔猪肝脏代谢的关键蛋白筛选[D]. 郑州:河南农业大学, 2015.
[38] GAN Z D, WEI W Y, WU J M, et al. Resveratrol and curcumin improve intestinal mucosal integrity and decrease m⁶A RNA methylation in the intestine of weaning piglets[J]. ACS Omega, 2019, 4(17):17438-17446.
[39] DOLARA P, LUCERI C, DE FILIPPO C, et al. Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats[J]. Mutat Res, 2005, 591(1-2):237-246.
[40] ZHANG J Q, XIONG W, LI Z H, et al. Effects of rutin on growth, intestinal antioxidant capacity and lipid metabolism in weaned mice[J]. Journal of Nanjing Agricultural University, 2022, 45(2):368-376. (in Chinese)
张佳琦, 熊玮, 黎智华, 等. 芦丁对断奶仔鼠生长、肠道抗氧化及脂代谢的影响[J]. 南京农业大学学报, 2022, 45(2):368-376.
[41] SINGH R, CHANDRASHEKHARAPPA S, BODDULURI S R, et al. Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway[J]. Nat Commun, 2019, 10(1):89.
[42] HEIDEBRECHT H J, KULOZIK U. Fractionation of casein micelles and minor proteins by microfiltration in diafiltration mode. Study of the transmission and yield of the immunoglobulins IgG, IgA and IgM[J]. Int Dairy J, 2019, 93:1-10.
[43] WANG Y H, BAI C X, YANG K K, et al. Mechanism of green tea powder improving intestinal barrier of immune stressed mice[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(S1):136-142. (in Chinese)
王元红, 白彩霞, 杨侃侃, 等. 绿茶粉改善免疫应激小鼠肠道屏障的作用机制[J]. 华北农学报, 2019, 34(S1):136-142.
[44] LU Y, LI X R, LIU S S, et al. Toll-like receptors and inflammatory bowel disease[J]. Front Immunol, 2018, 9:72.
[45] SHAO X, SUN C Z, TANG X, et al. Anti-inflammatory and intestinal microbiota modulation properties of Jinxiang garlic (Allium sativum L.) polysaccharides toward dextran sodium sulfate-induced colitis[J]. J Agric Food Chem, 2020, 68(44):12295-12309.
[46] NIU J H, YUAN J, WEI R, et al. Protective effect and mechanism study of jujube polysaccharides on intestinal immune barrier in mice[J]. Science and Technology of Food Industry, 2021, 42(4):295-300, 306. (in Chinese)
牛佳卉, 袁静, 魏然, 等. 红枣多糖对小鼠肠道免疫屏障的保护作用及机制研究[J]. 食品工业科技, 2021, 42(4):295-300, 306.
[47] QU J L, HUANG P, ZHANG L, et al. Hepatoprotective effect of plant polysaccharides from natural resources:a review of the mechanisms and structure-activity relationship[J]. Int J Biol Macromol, 2020, 161:24-34.
[48] MAO S H, FENG D D, WANG X, et al. Magnolol protects against acute gastrointestinal injury in sepsis by down-regulating regulated on activation, normal T-cell expressed and secreted[J]. World J Clin Cases, 2021, 9(34):10451-10463.
[49] YE J, YE C Q, HUANG Y Y, et al. Ginkgo biloba sarcotesta polysaccharide inhibits inflammatory responses through suppressing both NF-κB and MAPK signaling pathway[J]. J Sci Food Agr, 2019, 99(5):2329-2339.
[50] SUN L, XU G Q, DONG Y Y Y, et al. Quercetin protects against lipopolysaccharide-induced intestinal oxidative stress in broiler chickens through activation of nrf2 pathway[J]. Molecules, 2020, 25(5):1053.
[51] MA G X, KIMATU B M, YANG W J, et al. Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential[J]. J Food Sci, 2020, 85(9):2822-2831.
[52] SOKOL H. Probiotics and antibiotics in IBD[J]. Dig Dis, 2014, 32(S1):10-17.
[53] KRAIMI N, LORMANT F, CALANDREAU L, et al. Microbiota and stress:a loop that impacts memory[J]. Psychoneuroendocrinology, 2022, 136:105594.
[54] DING S J, JIANG H M, FANG J, et al. Regulatory effect of resveratrol on inflammation induced by lipopolysaccharides via reprograming intestinal microbes and ameliorating serum metabolism profiles[J]. Front Immunol, 2021, 12:777159.
[55] LAI P K, ROY J. Antimicrobial and chemopreventive properties of herbs and spices[J]. Curr Med Chem, 2004, 11(11):1451-1460.
[56] SICHERT A, CORZETT C H, SCHECHTER M S, et al. Verrucomicrobia use hundreds of enzymes to digest the algal polysaccharide fucoidan[J]. Nat Microbiol, 2020, 5(8):1026-1039.
[57] LEE H C, JENNER A M, LOW C S, et al. Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota[J]. Res Microbiol, 2006, 157(9):876-884.
[58] THU T V, LOH T C, FOO H L, et al. Effects of liquid metabolite combinations produced by Lactobacillus plantarum on growth performance, faeces characteristics, intestinal morphology and diarrhoea incidence in postweaning piglets[J]. Trop Anim Health Prod, 2011, 43(1):69-75.
[59] TREMAROLI V, BÄCKHED F. Functional interactions between the gut microbiota and host metabolism[J]. Nature, 2012, 489(7415):242-249.
[60] MEI H D, LI Y F, TIAN Q, et al. Effects of magnolol on nutrient apparent digestibility, serum biochemical indices, main fecal microorganism numbers and their metabolite contents of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2022, 34(8):4919-4931. (in Chinese)
梅华迪, 李袁飞, 田琦, 等. 厚朴酚对断奶仔猪营养物质表观消化率、血清生化指标、粪便主要微生物数量及其代谢产物含量的影响[J]. 动物营养学报, 2022, 34(8):4919-4931.