鸡滑液囊支原体对常用抗菌药流行病学临界值的建立

doi:10.11843/j.issn.0366-6964.2024.06.041

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (6): 2701-2715.doi: 10.11843/j.issn.0366-6964.2024.06.041

鸡滑液囊支原体对常用抗菌药流行病学临界值的建立

邹紫莹(), 黄安雄, 阮紫涵, 郝海红*()

农业微生物资源发掘与利用全国重点实验室(华中农业大学), 国家兽药残留基准实验室(华中农业大学), 农业部兽药残留检测重点实验室(华中农业大学), 国家兽药安全评价实验室(华中农业大学), 农业部畜禽产品质量安全风险评估实验室(武汉), 武汉 430070

收稿日期:2023-07-18 出版日期:2024-06-23 发布日期:2024-06-28
通讯作者: 郝海红 E-mail:zzy15179469958@163.com;haohaihong@mail.hzau.edu.cn
作者简介:邹紫莹(1999-)，女，江西抚州人，硕士生，主要从事兽医药理学和抗菌耐药性研究，E-mail: zzy15179469958@163.com
基金资助:
国家重点研发计划(2021YFD1800600);国家自然科学基金(32172914);中央高校自主创新基金(2662022DKYJC005);湖北省自然科学基金(2021CFA016);现代农业产业技术体系项目(CARS-41)

Establishment on Commonly Used Antimicrobials Epidemiological Cut-off Values of Mycoplasma synoviae

Ziying ZOU(), Anxiong HUANG, Zihan RUAN, Haihong HAO*()

State Key Laboratory of Agricultural Microbiology (HZAU), National Reference Laboratory of Veterinary Drug Residues (HZAU), The Key Laboratory for the Detection of Veterinary Drug Residues, Ministry of Agriculture(HZAU), National Laboratory for Safety Evaluation of Veterinary Drugs(HZAU), Laboratory of Quality & Safety Risk Assessment for Livestock and Poultry Products (Wuhan), Wuhan 430070, China

Received:2023-07-18 Online:2024-06-23 Published:2024-06-28
Contact: Haihong HAO E-mail:zzy15179469958@163.com;haohaihong@mail.hzau.edu.cn

摘要/Abstract

摘要：

鸡滑液囊支原体(Mycoplasma synoviae，MS)是现在规模化禽养殖业中危害最大的支原体之一，同时也出现了严重的耐药性。为了解鸡滑液囊支原体对各种药物的耐药现状。本课题参考CLSI和EUCAST中制定流行病学/野生型临界值(epidemiological/wildtype cut-off values，ECOFFs/CO_WT)的方法，通过查找国内外相关文献报道，收集汇总近5年不同地区、不同来源的MS对各种药物的最小抑菌浓度(minimum inhibitory concentration，MIC)数据，应用肉眼观察法、ECOFFinder软件分析法和正态化耐药解释法(NRI)综合分析从而得到ECOFFs，随后计算相应的耐药率。结果显示，MS对泰乐菌素、替米考星、泰万菌素、多西环素、土霉素、金霉素、泰妙菌素、林可霉素、氟苯尼考和大观霉素的ECOFF值分别为1、0.5、0.5、2、4、2、2、2、8和4 μg·mL^-1。在CLSI和EUCAST缺乏MS对各种抗菌药的敏感性折点下，本课题为临床合理用药，耐药性工作的开展，减少MS耐药性的产生以及治疗与防控提供了理论基础与科学依据。

关键词: 鸡滑液囊支原体, 流行病学临界值, 耐药性, ECOFFinder软件, 正态化耐药解释法

Abstract:

Mycoplasma synoviae (MS) is one of the most detrimental mycoplasmas in the large-scale poultry industry. Meanwhile, the development of antimicrobial resistance to MS is serious currently. In order to understand the current situation of antimicrobial resistance to MS, the research was conducted in reference to the method of establishing epidemiological/wildtype cut-off values (ECOFFs/CO_WT) in CLSI and EUCAST by collecting and summarizing MIC from different regions and sources in recent 5 years after searching relevant literature reports at home and abroad, and then the ECOFFs of various antimicrobial agents to MS were obtained through comprehensive analysis using visual estimation, ECOFFFinder software analysis and Normalized Drug Resistance Interpretation (NRI). Subsequently, the corresponding drug resistance rates were calculated. It showed that the ECOFFs of MS to tylosin, tilmicosin, tylvalosin, doxycycline, oxytetracycline, chlortetracycline, tiamulin, lincomycin, florfenicol and spectinomycin were 1, 0.5, 0.5, 2, 4, 2, 2, 2, 8 and 4 μg·mL^-1 respectively. In absence of sensitive breakpoint of MS in CLSI and ECUAST, the theoretical basis and scientific evidence are provided by this research for the rational use of drugs in clinical practice, development of antimicrobial resistance work for monitoring and reducing it, and also beneficial for the treatment and control of MS.

Key words: Mycoplasma synoviae, epidemiological cut-off values, drug resistance, ECOFFinder software, normalized drug resistance interpretation (NRI)

中图分类号:

S852.62

邹紫莹, 黄安雄, 阮紫涵, 郝海红. 鸡滑液囊支原体对常用抗菌药流行病学临界值的建立[J]. 畜牧兽医学报, 2024, 55(6): 2701-2715.

Ziying ZOU, Anxiong HUANG, Zihan RUAN, Haihong HAO. Establishment on Commonly Used Antimicrobials Epidemiological Cut-off Values of Mycoplasma synoviae[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2701-2715.

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药物种类 Drug classification	药物名称 Drugs	菌株总数 Number	来源地区 Data source	相关文献 Related literature
大环内酯类	泰乐菌素Tylosin	453	欧洲、亚洲	[26-34]
Macrolides	替米考星Tilmicosin	448	欧洲、亚洲、非洲	[26-34]
	泰万菌素Tylvalosin	238	欧洲	[26, 28, 32-34]
四环素类	多西环素Doxycycline	198	欧洲、亚洲	[27-29, 31]
Tetracyclines	土霉素Oxytetracycline	301	欧洲、亚洲、大洋洲、非洲	[26-29, 31-32, 34]
	金霉素Chlortetracycline	133	欧洲、亚洲、大洋洲、非洲	[26, 32, 34]
氟喹诺酮类	恩诺沙星Enrofloxacin	217	欧洲、亚洲	[28-31]
Fluoroquinolones
截短侧耳素类	泰妙菌素Tiamulin	245	欧洲、亚洲	[26-31]
Pleuromutilins
林可酰胺类	林可霉素Lincomycin	305	欧洲、亚洲、非洲、大洋洲	[26, 31-34]
Lincomamides
氯霉素类	氟苯尼考Florfenicol	321	欧洲、亚洲、非洲、大洋洲	[26, 28, 31-32, 34]
Chloramphenicols
氨基糖苷类	大观霉素Spectinomycin	139	欧洲、亚洲、非洲、大洋洲	[26, 32, 34]
Aminoglycosides	新霉素Neomycin	118	欧洲、亚洲、非洲、大洋洲	[32, 34]

药物 Drugs	MIC₅₀/(μg·mL^-1)	MIC₉₀/(μg·mL^-1)	ECOFF _V_isual /(μg·mL^-1)	ECOFF _ECOFF_inder/(μg·mL^-1)	ECOFF _NRI/(μg·mL^-1)	ECOFF _Final/(μg·mL^-1)	耐药率/% DR
泰乐菌素TYL	0.25	2	1	1	0.25	1	11.0
替米考星TIL	0.5	128	0.5	0.5	4	0.5	46.9
泰万菌素TVL	0.25	0.5	0.5	0.5	0.5	0.5	4.2
多西环素DOX	0.5	2	2	2	2	2	5.6
土霉素OTC	1	2	4	4	8	4	1.4
金霉素CTC	1	4	2	2	2	2	17.3
恩诺沙星ENR	32	32	/	/	/	/	/
泰妙菌素TML	0.25	1	2	2	0.125	2	0.4
林可霉素LCM	1	128	2	2	4	2	18.0
氟苯尼考FFC	2	8	8	8	16	8	0
大观霉素SPT	2	4	4	4	8	4	2.2
新霉素NEM	32	128	128	128	128	/	/

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鸡滑液囊支原体对常用抗菌药流行病学临界值的建立

Establishment on Commonly Used Antimicrobials Epidemiological Cut-off Values of Mycoplasma synoviae

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MIC/(μg·mL^-1)	抗菌药物Antimicrobial agents
MIC/(μg·mL^-1)	TYL	TIL	TVL	DOX	OTC	CTC	ENR	TML	LCM	FFC	SPT	NEM
0.004	3^a							3^a
0.008								9
0.016	14	7						26
0.031	43	7						14
0.063	50	10	6	4^a	1			14^a
0.125	28	14	2	2	2			15
0.25	201^a	145^a	200^a	20	45^a	22^a		57	40^a	24^a	3^a
0.5	45	55	20	89	57	40		78	69	7	10
1	19	56	1	59	108	31	4	20	120	88	39
2	32	27		13	64	17	11	8	21	97	67
4	4	19	8	8	19	10	17	0	12	62	17	2
8	3	19	1	3	5	4	20	0	0	43	3	9
16	3	13				9^b	36	0	0			23
32	4	12					129^b	1	0			52
64	2	15^b							2^b			17
128	2^b	48^b							41			15^b
256		1^b
总计Total	453	448	238	198	348	133	217	245	305	321	139	118