1株鸡滑液囊支原体的分离鉴定与全基因组分析

doi:10.11843/j.issn.0366-6964.2022.08.024

摘要/Abstract

摘要： 鸡滑液囊支原体（Mycoplasma synoviae，MS）能引起家禽呼吸道疾病、传染性滑膜炎、蛋壳顶端异常及生长迟缓等，给全球养禽业带来巨大的经济损失。然而不同国家和地区MS的耐药性和致病性存在差异，因此本研究旨在挖掘福建MS菌株的特征。作者采集福建省疑似患MS的病鸡关节腔内容物，对菌株进行分离鉴定，并利用三代联合二代测序技术对其进行全基因组测序。结果显示，该分离株病原特性稳定，已保藏至中国典型培养物保藏中心，保藏编号为CCTCC No.：M 2021210，并将其命名为MS-FJ01。MS-FJ01基因组全长为795 381 bp，GC含量为28.39%；预测编码基因数为704个，占整个基因组的90.48%；并在COG、GO和KEGG数据库分别注释到476、382、425个基因。通过各数据库的分析，注释了40个耐药相关基因，4个碳水化合物相关酶基因，136个病原与宿主互作相关基因，47个毒力因子相关基因，106个膜转运蛋白相关基因，25个限制修饰相关基因。通过与MS HN01和MS 5-9基因组进一步比较发现，MS-FJ01与这两个中国菌株均存在良好的共线性关系，且与MS 5-9的亲缘关系更近。本研究阐明了福建MS菌株的基因组结构、通过相关数据库的分析，预测和注释了其基因的功能，为深入研究MS提供参考依据。

关键词: 鸡滑液囊支原体, 分离鉴定, 全基因组测序, 基因功能注释分析

Abstract: Mycoplasma synoviae (MS) can cause respiratory diseases, infectious synovitis, abnormal eggshell top and growth retardation in poultry, and bring huge economic losses to the global poultry industry. However, there are differences in drug resistance and pathogenicity of MS in different countries and regions. Therefore, this study aims to explore the characteristics of MS strains in Fujian. The contents of the articular cavity of chickens suspected of suffering from MS in Fujian were collected, the strains were isolated and identified, and the whole genome was sequenced by third-generation combined with second-generation sequencing technology. The results showed that the pathogenic characteristics of the isolate were stable and had been deposited in China center for type culture collection. The preservation number was CCTCC No.:M 2021210 and named MS-FJ01. The total length of MS-FJ01 genome was 795 381 bp and the content of GC was 28.39%; The predicted number of coding genes was 704, accounting for 90.48% of the whole genome; 476, 382 and 425 genes were annotated in COG, GO and KEGG databases respectively. Through the analysis of each data, 40 drug resistance related genes, 4 carbohydrate related enzyme genes, 136 pathogen host interaction related genes, 47 virulence factor related genes, 106 membrane transporter related genes and 25 restriction modification related genes were annotated. Through further comparison with MS HN01 and MS 5-9 genomes, it was found that MS-FJ01 had a good collinear relationship with both Chinese strains, and was closer to MS 5-9. This study clarified the genomic structure of Fujian MS strain, predicted and annotated the function of its gene through the analysis of relevant databases, to provide a reference basis for the further study of MS.

Key words: Mycoplasma synoviae, isolation and identification, whole genome sequencing, gene function annotation analysis

中图分类号:

S852.62

许李锋, 贾晨宇, 陈福再, 方梦园, 刘孝丹, 陈吉龙, 李训良. 1株鸡滑液囊支原体的分离鉴定与全基因组分析[J]. 畜牧兽医学报, 2022, 53(8): 2663-2676.

XU Lifeng, JIA Chenyu, CHEN Fuzai, FANG Mengyuan, LIU Xiaodan, CHEN Jilong, LI Xunliang. Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2663-2676.

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