副猪嗜血杆菌qseB、qseC双基因缺失株的构建及生物学特性

doi:10.11843/j.issn.0366-6964.2022.02.019

摘要/Abstract

摘要： QseBC双组分系统（QseBC two-component regulatory systern）与细菌中的群体感应相关，是肠杆菌科和巴斯德氏菌科中的全局性毒力调控因子，但在副猪嗜血杆菌（Glaesserella parasuis）中的作用还不清楚。本研究利用自然转化法构建SC1401的双基因缺失株（ΔqseBC）。比较缺失株（ΔqseBC）与野毒株（SC1401）的抗血清杀菌能力、生物膜形成、抗生素耐药性以及压力耐受能力。结果表明：ΔqseBC的生物被膜形成能力明显弱于SC1401。缺失株ΔqseBC的在猪血清中的存活率为55.12%，低于野毒株SC1401（83.76%）。ΔqseBC对恩诺沙星、庆大霉素、头孢噻呋和青霉素的最小抑制浓度（MIC）和最小杀菌浓度（MBC）明显下降。ΔqseBC对39和42℃高温，1、2、4、8和16 mmol·L^-1 H₂O₂，以及50、100和150 mmol·L^-1 NaCl表现出更高的敏感性。综上表明，QseBC双组分系统在副猪嗜血杆菌的血清抗性、抗生素抗性和胁迫耐受性中发挥了重要作用。QseBC可能与副猪嗜血杆菌的致病能力密切有关，但其具体机制还需要进一步研究。

关键词: QseBC, 抗血清杀菌, 生物被膜, 抗生素耐药性, 压力耐受

Abstract: The QseBC two-component system (TCS) is related to quorum sensing of bacteria. As a global regulator of virulence in Enterobacteriaceae and Pasteurellaceae, its role still unclear in Glaesserella (Haemophilus) parasuis. In this study, the double gene deletion mutant ΔqseBC of SC1401 was constructed by natural transformation method. The serum resistance ability, biofilm formation, antibiotic resistance and stress tolerance of ΔqseBC mutant strain and wild strain SC1401 were measured. Results were as follows:the biofilm-forming ability of ΔqseBC was significantly weaker than that of the wild strain SC1401. The survival rate of the ΔqseBC mutant in porcine serum was 55.12%, which was lower than that of the wild strain SC1401 (83.76%). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ΔqseBC mutant strain to enrofloxacin, gentamicin, ceftiofur and penicillin were significantly decreased. The ΔqseBC mutant strain showed high sensitivity to high temperature (39 and 42℃), hydrogen peroxide (1, 2, 4, 8 and 16 mmol·L^-1) and NaCl (50, 100 and 150 mmol·L^-1). The results showed that the TCS QseBC plays an important role in the serum resistance, antibiotic resistance and stress tolerance of Glaesserella parasuis. The above findings revealed that QseBC might be closely related to the pathogenicity of Glaesserella parasuis, but further studies are needed to determine the specific mechanism.

Key words: QseBC, serum resistance, biofilm, antibiotic resistance, stress tolerance

中图分类号:

S852.61

何绿琴, 闫雪锋, 文心田, 曹三杰, 黄小波, 伍锐, 赵勤, 文翼平. 副猪嗜血杆菌qseB、qseC双基因缺失株的构建及生物学特性[J]. 畜牧兽医学报, 2022, 53(2): 529-537.

HE Lüqin, YAN Xuefeng, WEN Xintian, CAO Sanjie, HUANG Xiaobo, WU Rui, ZHAO Qin, WEN Yiping. Construction and Biological Characteristic of Glaesserella parasuis Strain with qseB, qseC Double Gene Deletion[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 529-537.

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