7株鸡滑液囊支原体四川分离株全基因组分析

doi:10.11843/j.issn.0366-6964.2022.02.017

摘要/Abstract

摘要： 旨在了解滑液囊支原体（Mycoplasma synoviae，MS）遗传多样性，本研究对7株MS四川分离株进行全基因组测序及生物信息学分析。采用Illumina HiSeq平台和PE文库结合方式全基因组测序，运用各数据库注释，用MEGAX软件将7株MS与NCBI收录的8株MS全基因比较。结果显示，分离自同一鸡场的4株MS分离株MS251、MS254、MS221、MS231的基因数、种类、毒力因子数相同或差异小，而分离自3个不同鸡场的MS分离株MS12H、MS1G、MSK1的基因数、毒力因子数存在较大差异；将该7株MS四川分离株与NCBI公布的8株MS分离株比较圈图，发现四川分离株与巴西MS53、美国WVU-1853、澳大利亚86079/7 NS，以及疫苗株MS-H差异较大，而与河南HN01最相近。不同场间和同场间四川分离株都存在遗传多样性。本研究为研究MS分离株的致病机制、开发MS亚单位疫苗和建立特异性检测方法提供了理论依据。

关键词: 鸡滑液囊支原体, 全基因组, 预测结果, 进化分析, 线性分析

Abstract: In order to understand the genetic diversity of Mycoplasma synoviae (MS), seven MS strains isolated from Sichuan were sequenced and analyzed by bioinformatics. Illumina HiSeq platform and PE library were combined for whole genome sequencing, and the whole genome were annotated by several database. The complete genomes of 7 MS isolates were compared with those of 8 MS strains included in NCBI by MEGAX software. The results showed that the gene numbers, species and virulence factors of 4 MS strains MS251, MS254, MS221, MS231 isolated from the same chicken farm were the same or had little difference, while the gene number and virulence factor numbers of MS isolates MS12H, MS1G and MSK1 isolated from other three different chicken farms were quite different. Comparing the 7 MS Sichuan isolates with the 8 MS strains published by NCBI, it was found that the Sichuan MS isolates were significantly different from Brazilian MS53, American WVU-1853, Australian 86079/7 NS and vaccine strain MS-H, however, most similar to Henan HN01, China. There was genetic diversity among MS isolates from different farms and within the same farm. This study provided a necessary theoretical basis for further study of the pathogenic mechanism of MS isolates, the development of MS subunit vaccine and the establishment of specific detection technology.

Key words: Mycoplasma synovium, whole genome, prediction results, evolutionary analysis, linear analysis

中图分类号:

S852.62

刘丽佳, 王誉, 张焕容, 王嘉博. 7株鸡滑液囊支原体四川分离株全基因组分析[J]. 畜牧兽医学报, 2022, 53(2): 505-519.

LIU Lijia, WANG Yu, ZHANG Huanrong, WANG Jiabo. Whole Gene Analysis of 7 Mycoplasma synoviae Strains from Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 505-519.

参考文献 27

[1]	PASCUCCI S, MAESTRINI N, GOVONI S, et al. Mycoplasma synoviae in the guinea-fowl[J]. Avian Pathol, 1976, 5(4):291-297.
[2]	KLEVEN S H. Mycoplasmas in the etiology of multifactorial respiratory disease[J]. Poult Sci, 1998, 77(8):1146-1149.
[3]	MOREIRA F A, CARDOSO L, COELHO A C. Epidemiological survey on Mycoplasma synoviae infection in Portuguese broiler breeder flocks[J]. Vet Ital, 2015, 51(2):93-98.
[4]	FEBERWEE A, DE WIT J J, LANDMAN W J M. Induction of eggshell apex abnormalities by Mycoplasma synoviae:field and experimental studies[J]. Avian Pathol, 2009, 38(1):77-85.
[5]	陈秀红, 郭亚男, 司朵朵, 等. 宁夏地区鸡滑液囊支原体病流行病学调查与分析[J]. 中国预防兽医学报, 2020, 42(3):234-238.CHEN X H, GUO Y N, SI D D, et al. Epidemiological investigation and analysis of Mycoplasma synoviae in infection in chickens in Ningxia region[J]. Chinese Journal of Preventive Veterinary Medicine, 2020, 42(3):234-238. (in Chinese)
[6]	马爽, 郭莉莉, 宋新宇, 等. 鸡滑液囊支原体感染的流行病学调查与分析[J]. 中国家禽, 2016, 38(23):68-71.MA H, GUO L L, SONG X Y, et al. Epidemiological investigation and analysis of Mycoplasma synovial infection in chickens[J]. China Poultry, 2016, 38(23):68-71. (in Chinese)
[7]	SUN S K, LIN X, CHEN F, et al. Epidemiological investigation of Mycoplasma synoviae in native chicken breeds in China[J]. BMC Vet Res, 2017, 13(1):115.
[8]	VASCONCELOS A T R, FERREIRA H B, BIZARRO C V, et al. Swine and poultry pathogens:the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae[J]. J Bacteriol, 2005, 187(16):5568-5577.
[9]	MAY M A, KUTISH G F, BARBET A F, et al. Complete genome sequence of Mycoplasma synoviae strain WVU 1853T[J]. Genome Announc, 2015, 3(3):e00563-15.
[10]	ZHU L, SHAHID M A, MARKHAM J, et al. Comparative genomic analyses of Mycoplasma synoviae vaccine strain MS-H and its wild-type parent strain 86079/7 NS:implications for the identification of virulence factors and applications in diagnosis of M. synoviae[J]. Avian Pathol, 2019, 48(6):537-548.
[11]	XU B, LIU R, TAO L H, et al. Complete genome sequencing of Mycoplasma synoviae strain HN01, isolated from chicken in Henan province, China[J]. Microbiol Resour Announc, 2020, 9(6):e01480-19.
[12]	MARKHAM J F, SCOTT P C, WHITHEAR K G. Field evaluation of the safety and efficacy of a temperature-sensitive Mycoplasma synoviae live vaccine[J]. Avian Dis, 1998, 42(4):682-689.
[13]	田亚琴, 文玉康, 王豪举, 等. 鸡滑液囊支原体疫苗研究进展[J]. 中国家禽, 2020, 42(9):96-102.TIAN Y Q, WEN Y K, WANG H J, et al. Research progress in the development of Mycoplasma synoviae vaccine[J]. China Poultry, 2020, 42(9):96-102. (in Chinese)
[14]	李凡, 张焕容, 陈云霞. 川西地区鸡滑液囊支原体分离株的部分生物学特性及其VlhA基因遗传进化分析[J]. 中国预防兽医学报, 2020, 42(2):122-127.LI F, ZHANG H R, CHEN Y X. Partial biological charicteristics of Mycoplasma synoviae isolates from western Sichuan analysis of it VIhA gene[J]. Chinese Journal of Preventive Veterinary Medicine, 2020, 42(2):122-127. (in Chinese)
[15]	CATANIA S, BILATO D, GOBBO F, et al. Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection[J]. Avian Dis, 2010, 54(2):961-964.
[16]	NOORMOHAMMADI A H, HEMMATZADEH F, WHITHEAR K G. Safety and efficacy of the Mycoplasma synoviae MS-H vaccine in turkeys[J]. Avian Dis, 2007, 51(2):550-554.
[17]	石晓磊. 鸡滑液囊支原体的分离鉴定及其活疫苗免疫效果的评价[D]. 杨凌:西北农林科技大学, 2018.SHI X L. Isolation and identification of Mycoplasma synoviae and evaluation of the effect of live-vaccine[D]. Yangling:Northwest A&F University, 2018. (in Chinese)
[18]	URBAN M, CUZICK A, RUTHERFORD K, et al. PHI-base:a new interface and further additions for the multi-species pathogen-host interactions database[J]. Nucleic Acids Res, 2017, 45(D1):D604-D610.
[19]	KLEINHEINZ K A, JOENSEN K G, LARSEN M V. Applying the ResFinder and VirulenceFinder web-services for easy identification of acquired antibiotic resistance and E. coli virulence genes in bacteriophage and prophage nucleotide sequences[J]. Bacteriophage, 2014, 4(2):e27943.
[20]	NOORMOHAMMADI A H, MARKHAM P F, DUFFY M F, et al. Multigene families encoding the major hemagglutinins in phylogenetically distinct mycoplasmas[J]. Infect Immun, 1998, 66(7):3470-3475.
[21]	EL-GAZZAR M M, WETZEL A N, RAVIV Z. The genotyping potential of the Mycoplasma synoviae vlhA gene[J]. Avian Dis, 2012, 56(4):711-719.
[22]	SUYAMA M, BORK P. Evolution of prokaryotic gene order:genome rearrangements in closely related species[J]. Trends Genet, 2001, 17(1):10-13.
[23]	ZHU L, SHAHID M A, MARKHAM J, et al. Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution[J]. BMC Genomics, 2018, 19(1):117.
[24]	MONNET V. Bacterial oligopeptide-binding proteins[J]. Cell Mol Life Sci, 2003, 60(10):2100-2114.
[25]	GARDAN R, BESSET C, GUILLOT A, et al. The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9[J]. J Bacteriol, 2009, 191(14):4647-4655.
[26]	MORAES P M R O, SEYFFERT N, SILVA W M, et al. Characterization of the Opp peptide transporter of Corynebacterium pseudotuberculosis and its role in virulence and pathogenicity[J]. BioMed Res Int, 2014, 2014:489782.
[27]	SABHARWAL N, VARSHNEY K, RATH P P, et al. Biochemical and biophysical characterization of nucleotide binding domain of Trehalose transporter from Mycobacterium tuberculosis[J]. Int J Biol Macromol, 2020, 152:109-116.