75株蛋鸡源沙门菌的MLST分型与耐药性分析

doi:10.11843/j.issn.0366-6964.2022.05.030

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (5): 1626-1631.doi: 10.11843/j.issn.0366-6964.2022.05.030

75株蛋鸡源沙门菌的MLST分型与耐药性分析

王喜^1,2, 李珂¹, 李廷翠^1,2, 严红亚¹, 赵蓉¹, 常志顺¹, 廖明³, 孙敏华^3*, 信爱国^1*

1. 云南省畜牧兽医科学院养禽与禽病研究所, 昆明 650224;
2. 云南农业大学动物医学院, 昆明 650201;
3. 广东省农业科学院动物卫生研究所, 广州 510642

收稿日期:2021-06-10 出版日期:2022-05-23 发布日期:2022-05-25
通讯作者: 信爱国,主要从事动物传染病应用基础研究,E-mail:aiguo_xin@hotmail.com;孙敏华,主要从事家禽疫病防控研究,E-mail:hql562713@163.com
作者简介:王喜(1994-),男,贵州兴义人,硕士生,主要从事兽医公共卫生学研究,E-mail:1510895059@qq.com;李珂(1987-),男,云南开远人,硕士,主要从事家禽疫病诊断及防治研究,E-mail:johnsonlike@163.com。
基金资助:
云南省廖明专家工作站(202105AF150077)；云南省“万人计划”产业技术领军人才专项(YNWR-CYJS-2018-047)

MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens

WANG Xi^1,2, LI Ke¹, LI Tingcui^1,2, YAN Hongya¹, ZHAO Rong¹, CHANG Zhishun¹, LIAO Ming³, SUN Minhua^3*, XIN Aiguo^1*

1. Department of Poultry Husbandry and Disease Research, Yunnan Animal Science and Veterinary Institute, Kunming 650224, China;
2. College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China;
3. Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China

Received:2021-06-10 Online:2022-05-23 Published:2022-05-25

摘要/Abstract

摘要： 旨在掌握2017年7月至2019年5月期间云南地区蛋鸡源沙门菌血清型、药物敏感性及毒力基因携带等基本情况。无菌采集发病鸡肝组织，共分离到沙门菌75株，对分离株进行MLST分型、药物敏感性及相关耐药基因和毒力基因检测。结果显示，MLST鉴定到ST78序列型鸡伤寒沙门菌54株(72.00%)、ST92序列型鸡白痢沙门菌21株(28.00%)；分离株对青霉素的耐药率为100%，对其它抗生素的耐药率分别为: 四环素26.67%、强力霉素26.67%、复方新诺明22.67%、阿莫西林18.67%、氨苄西林16.00%、恩诺沙星14.67%、链霉素8.00%、环丙沙星2.67%、庆大霉素1.33%，共存在7种耐药谱型，28.00%的菌株表现为多重耐药，且集中于鸡伤寒沙门菌；耐药基因tetA、sul2和blaTEM的检出率分别为26.67%、10.67%和8.00%；毒力基因mogA、mgtC、bcfA、araB、stn和spvC的检出率均高达100%，而spvB的检出率为 89.33%。结果表明，鸡伤寒沙门菌和鸡白痢沙门菌为云南地区蛋鸡源沙门菌主要流行血清型，多重耐药情况严重，耐药基因与毒力基因检出率较高。

关键词: 蛋鸡源沙门菌, MLST, 耐药性, 毒力基因, 云南

Abstract: The aim of this study was to investigate the serotypes, drug sensitivity and virulence genes of Salmonella isolated from laying hens in Yunnan between July 2017 and May 2019. A total of 75 Salmonella strains were isolated from the liver tissues of sick chickens. MLST serotyping, drug sensitivity, related resistance genes and virulence genes were detected. A total of 54 strains (72.00%) and 21 strains (28.00%) were determined to be ST78 and ST92, respectively, by MLST. The resistance rate to penicillin was 100%, and the resistance rates were 26.67% to tetracycline, 26.67% to doxycycline, 22.67% to sulfamethoxazole, 18.67% to amoxicillin, 16.00% to ampicillin, 14.67% to enrofloxacin, 8.00% to streptomycin, 2.67% to ciprofloxacin, and 1.33% to gentamicin. There were seven profiles of drug resistance spectrum, with 28.00% of the strains (mainly S. Gallinarum) showing multiple resistance. The detection rates of resistance genes tetA, sul2 and blaTEM were 26.67%, 10.67% and 8.00%, respectively. The virulence genes mogA, mgtC, bcfA, araB, stn and spvC were detected in all 75 strains, while the detection rate of spvB was 89.33%. The present study showed that S. Gallinarum and S. Pullorum were the main epidemic serotypes of Salmonella in laying hens in Yunnan, and that the multiple drug resistance was predominant due to the high prevalence of drug resistance genes and virulence genes harbored in the Salmonella strains.

Key words: Salmonella isolated from laying hens, MLST, drug resistance, virulence gene, Yunnan

中图分类号:

王喜, 李珂, 李廷翠, 严红亚, 赵蓉, 常志顺, 廖明, 孙敏华, 信爱国. 75株蛋鸡源沙门菌的MLST分型与耐药性分析[J]. 畜牧兽医学报, 2022, 53(5): 1626-1631.

WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.

参考文献 17

[1]	BENNASAR A,DE LUNA G,CABRER B,et al.Rapid identification of Salmonella typhimurium,S.enteritidis and S.virchow isolates by polymerase chain reaction based fingerprinting methods[J].Int Microbiol,2000,3(1):31-38.
[2]	ZHANG J L,WEI L J,KELLY P,et al.Detection of Salmonella spp.using a generic and differential FRET-PCR[J].PLoS One,2013,8(10):e76053.
[3]	张珍,施开创,王孝德,等.2015-2017年广西鸡源沙门氏菌耐药性与致病性的相关性分析[J].南方农业学报,2019,50(10):2350-2358.ZHANG Z,SHI K C,WANG X D,et al.Correlation between antimicrobial resistance and pathogenicity of Salmonella from chicken in Guangxi during 2015-2017[J].Journal of Southern Agriculture,2019,50(10):2350-2358.(in Chinese)
[4]	TARABEES R,ELSAYED M S A,SHAWISH R,et al.Isolation and characterization of Salmonella enteritidis and Salmonella typhimurium from chicken meat in Egypt[J].J Infect Dev Ctries,2017,11(4):314-319.
[5]	CHUANCHUEN R,PATHANASOPHON P,KHEMTONG S,et al.Susceptibilities to antimicrobials and disinfectants in Salmonella isolates obtained from poultry and swine in Thailand[J].J Vet Med Sci,2008,70(6):595-601.
[6]	CHUANCHUEN R,PADUNGTOD P.Antimicrobial resistance genes in Salmonella enterica isolates from poultry and swine in Thailand[J].J Vet Med Sci,2009,71(10):1349-1355.
[7]	王晶钰,董睿,王利勤,等.市售鲜鸡蛋中沙门氏菌的分离鉴定及毒力岛基因检测[J].食品科学,2012,33(16):154-158.WANG J Y,DONG R,WANG L Q,et al.Isolation,identification and pathogenicity island gene detection of Salmonella in commercial eggs[J].Food Science,2012,33(16):154-158.(in Chinese)
[8]	王德宁.鸡源沙门氏菌耐药性、致病性与毒力基因相关性分析[D].哈尔滨:东北农业大学,2014.WANG D N.Correlation analysis among drug-resistance,pathogenicity and virulence genes of Salmonella isolated from chickens[D].Harbin:Northeast Agricultural University,2014.(in Chinese)
[9]	程琼,庞瑞亮,王若晨,等.不同源沙门氏菌对小鼠致病力的比较与毒力基因检测[J].中国人兽共患病学报,2013,29(5):460-465.CHENG Q,PANG R L,WANG R C,et al.Comparative study on pathogenicity of Salmonella isolates from different sources of laboratory mice and the detection of their virulence genes[J].Chinese Journal of Zoonoses,2013,29(5):460-465.(in Chinese)
[10]	SHIVAPRASAD H L.Fowl typhoid and pullorum disease[J].Rev Sci Tech,2000,19(2):405-424.
[11]	郑之北,郑伟,汪皓秋,等.杭州地区多重耐药沙门氏菌的耐药特征[J].微生物学通报,2021,48(2):536-544.ZHENG Z B,ZHENG W,WANG H Q,et al.Characteristics and molecular subtyping of multidrug resistant Salmonella in Hangzhou[J].Microbiology China,2021,48(2):536-544.(in Chinese)
[12]	HAQUE A K M Z,AKTER M R,ISLAM S S,et al.Salmonella Gallinarum in small-scale commercial layer flocks:occurrence,molecular diversity and antibiogram[J].Vet Sci,2021,8(5):71.
[13]	LAW A,SOLANO O,BROWN C J,et al.Biosolids as a source of antibiotic resistance plasmids for commensal and pathogenic bacteria[J].Front Microbiol,2021,12:606409.
[14]	孙朋浩,邱荣超,李书宁,等.广州市生鲜鸡中肠炎沙门菌的监测及耐药基因的分子鉴定[J].畜牧兽医学报,2018,49(2):388-395.SUN P H,QIU R C,LI S N,et al.Monitoring of Salmonella enteritidis and molecular identification of antibiotic resistance gene in fresh chicken of Guangzhou city[J].Acta Veterinaria et Zootechnica Sinica,2018,49(2):388-395.(in Chinese)
[15]	AHMED A M,SHIMAMOTO T.Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt[J].Microbiol Immunol,2012,56(4):254-261.
[16]	徐耀辉,邓同炜,齐亚如,等.三黄种鸡场孵化死胚中沙门菌的分离鉴定与耐药性分析[J].畜牧兽医学报,2018,49(5):1081-1088.XU Y H,DENG T W,QI Y R,et al.Isolation,Identification and antimicrobial resistance of Salmonella from dead chicken embryos in Sanhuang breeder farms[J].Acta Veterinaria et Zootechnica Sinica,2018,49(5):1081-1088.(in Chinese)
[17]	王月,陈晓,王若南,等.浙江省临床分离沙门菌血清型、药物敏感性和毒力基因的研究[J].中华传染病杂志,2020,38(10):646-650.WANG Y,CHEN X,WANG R N,et al.study on the serotype,drug sensitivity and virulence genes of clinical isolates of Salmonella in Zhejiang province[J].Chinese Journal of Infectious Diseases,2020,38(10):646-650.(in Chinese)

75株蛋鸡源沙门菌的MLST分型与耐药性分析

MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens

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