吡啶甲酸铬对热应激肉鸡淋巴细胞钙离子浓度及免疫功能的影响

畜牧兽医学报

吡啶甲酸铬对热应激肉鸡淋巴细胞钙离子浓度及免疫功能的影响

韩爱云^1，2，张敏红^1*，冯京海¹

（1.中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室，北京 100193；2. 石家庄学院化工学院，石家庄 050035）

收稿日期:2012-05-15 出版日期:2013-02-23 发布日期:2013-02-23
通讯作者: 张敏红，博士，研究员，博士生导师，Tel：010-62815990，E-mail: minhong.zhang@yahoo.com
作者简介:韩爱云(1981-)，女，河北石家庄人，博士，讲师，主要从事营养与安全方面研究，E-mail: aiyunhan@yahoo.com.cn
基金资助:
国家“十二五”科技支撑计划项目（2012BAD39B02）；动物营养学国家重点实验室自主研究课题［2004DA125184(团)0807］；博士科研启动基金（10BJ002）

Effect of Chromium Picolinate on Ca²⁺ Concentration of Lymphocyte and Immune Response from Heat Stress Broiler Chickens

HAN Ai-yun¹^，2, ZHANG Min-hong^1*, FENG Jing-hai¹

（1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. School of Chemical Engineering,Shijiazhuang University,Shijiazhuang 050035, China）

Received:2012-05-15 Online:2013-02-23 Published:2013-02-23

摘要/Abstract

摘要：

旨在研究吡啶甲酸铬(CrPic)对热应激条件下肉鸡淋巴细胞钙离子浓度及免疫功能指标的影响。本研究选择1日龄AA肉公鸡300只，随机分为5个组（Ⅰ~Ⅴ），每组5个重复，每个重复12只鸡。在15日龄时，试验Ⅰ～Ⅳ组肉鸡分别饲喂含0、400、800、1 200 μg·kg^-1 Cr的日粮2周后，再进行日循环高温（27 ℃—35 ℃—27 ℃）处理2周，试验Ⅴ组肉鸡则维持恒温22 ℃作为适温对照组。结果显示，日粮补铬（800～1 200 μg·kg^-1）可显著降低热应激肉鸡血浆中的胰岛素水平，抑制淋巴细胞内钙离子浓度和IL-2浓度升高，提高淋巴细胞转化率。表明高温条件下，日粮添加CrPic可缓解热应激对肉鸡细胞免疫功能造成的负面影响。

Abstract:

The experiment was conducted to investigate the effect of chromium picolinate (CrPic) on Ca²⁺concentration of lymphocyte and immune response from heat stress broiler chickens. Three hundred 1-day-old Arbor Acres male broiler chickens were randomly allotted to 5 treatments (Ⅰ-Ⅴ), each treatment represented by 5 replicates of 12 birds each. At 15-day-old，the broilers in treatmentⅠ-Ⅳ were pre-fed 0, 400，800，1 200 μg·kg^-1 Cr of diet for 2 weeks, then they were housed in 3 controlled environment chambers with daily high cyclic temperature (27 ℃—35 ℃—27 ℃) for 14 d, the broilers in treatment Ⅴ were kept in 22 ℃ as the control group. The result shows that supplemental chromium (800-1 200 μg·kg^-1 Cr) decreased serum insulin concentration, supressed the increasement of the lymphocytes ［Ca²⁺］_iand IL-2 concentration, increased the lymphocyte proliferation. The results sugget that the CrPic could alleviate the negative effect of heat stress on the cellular immune response of heat stress broiler chickens.

中图分类号:

S831.4

韩爱云，张敏红，冯京海. 吡啶甲酸铬对热应激肉鸡淋巴细胞钙离子浓度及免疫功能的影响[J]. 畜牧兽医学报, doi: .

HAN Ai-yun, ZHANG Min-hong, FENG Jing-hai. Effect of Chromium Picolinate on Ca²⁺ Concentration of Lymphocyte and Immune Response from Heat Stress Broiler Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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参考文献

［1］DEEB N, CAHANER A. The effects of naked neck genotypes, ambient temperature, and feeding status and their interactions on body temperature and performance of broilers ［J］.Poult Sci, 1999, 78(10):1341-1346.

［2］AHMAD T, KHALID T, MUSHTAQ T, et al. Effect of potassium chloride supplementation in drinking water on broiler performance under heat stress conditions ［J］.Poult Sci, 2008, 87(7):1276-1280.

［3］MUSHTAQ T, SARWAR M, NAWAZ H, et al. Effect and interactions of dietary sodium and chloride on broiler starter performance (hatching to twenty-eight days of age) under subtropical summer conditions ［J］.Poult Sci, 2005, 84(11):1716-1722.

［4］RAO D S, GLICK B. Immunosuppressive action of heat in chickens ［J］.Proc Soc Exp Biol Med, 1970, 133(2):445-448.

［5］THAXTON P. Influence of temperature on the immune response of birds ［J］.Poult Sci, 1978, 57(5):1430-1440.

［6］SAMANTA S, HALDAR S, BAHADUR V, et al. Chromium picolinate can ameliorate the negative effects of heat stress and enhance performance, carcass and meat traits in broiler chickens by reducing the circulatory cortisol level ［J］.J Sci Food Agric, 2008, 88(5):787-796.

［7］张敏红，王丹莉，杜荣，等．高温下日粮铬水平对肉鸡生长性能和血液生化指标的影响［J］．动物营养学报，2002，14(1)：51-54.

［8］MOORE J W, MAHER M A, BANZ W J, et al. Chromium picolinate modulates rat vascular smooth muscle cell intracellular calcium metabolism ［J］.J Nutr, 1998, 128(2):180-184.

［9］DONG F, YANG X, SREEJAYAN N, et al. Chromium (D-phenylalanine)3 improves obesityinduced cardiac contractile defect in ob/ob mice ［J］.Obesity, 2007, 15(11):2699-2711.

［10］GENTRY L R, FERNANDEZ J M, WARD T L, et al. Dietary protein and chromium tripicolinate in Suffolk wether lambs: effects on production characteristics, metabolic and hormonal responses, and immune status ［J］.J Anim Sci, 1999, 77(5):1284-1294.

［11］PECHOVA A, PAVLATE L. Chromium as an essential nutrient: a review ［J］.Vet Med, 2007, 52(1):1-18.

［12］KEGLEY E B, SPEARS J W. Immune response, glucose metabolism, and performance of stressed feeder calves fed inorganic or organic chromium ［J］.J Anim Sci, 1995, 73(9):2721-2726.

［13］MOONSIE-SHAGEER S, MOWAT D N. Effect of level of supplemental chromium on performance, serum constituents, and immune status of stressed feeder calves ［J］.J Anim Sci, 1993, 71(1):232-238.

［14］BORGS P, MALLARD B A. Immune-endocrine interactions in agricultural species: chromium and its effect on health and performance ［J］.Domest Anim Endocrinol, 1998, 15(5):431-438.

［15］ANDERSON R A, POLANSKY M M, BRYDEN N A, et al. Effects of supplemental chromium on patients with symptoms of reactive hypoglycemia ［J］.Metabolism, 1987, 36(4):351-355.

［16］VINCENT J B. The biochemistry of chromium ［J］.J Nutr, 2000, 130(4):715-718.

［17］张敏红，张卫红，杜荣，等．补铬对高温环境下猪的铬代谢、生理生化反应和生产性能的影响［J］．畜牧兽医学报，2000，31（1）：1-8.

［18］ZHA L, ZENG J, SUN S, et al. Chromium (III) nanoparticles affect hormone and immune responses in heat-stressed rats ［J］.Biol Trace Elem Res, 2009, 129(1-3):157-169.

［19］BURTON J L, MALLARD B A, MOWAT D N. Effects of supplemental chromium on antibody responses of newly weaned feedlot calves to immunization with infectious bovine rhinotracheitis and parainfluenza 3 virus ［J］.Can J Vet Res, 1994, 58(2):148-151.

［20］MYERS M J, FARRELL D E, EVOCK-CLOVER C M, et al. Effect of recombinant growth hormone and chromium picolinate on cytokine production and growth performance in swine ［J］.Pathobiology, 1995, 63(5):283-287.

［21］BURTON J L, MALLARD B A, MOWAT D N. Effects of supplemental chromium on immune responses of periparturient and early lactation dairy cows ［J］.J Anim Sci, 1993, 71(6):1532-1539.

［22］LEE D N, YEN H T, SHEN T F, et al. Chromium-induced glucose uptake, superoxide anion production, and phagocytosis in cultured pulmonary alveolar macrophages of weanling pigs ［J］.Biol Trace Elem Res, 2000, 77(1):53-64.

［23］韩爱云，张敏红，左晓磊，等. 日循环高温对肉鸡淋巴细胞钙离子浓度、白介素-2水平及细胞周期的影响［J］.动物营养学报，2010，22(5):1243-1250.

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