共轭亚油酸影响边鸡胸肌脂类代谢的相关差异基因的筛选

doi:10.11843/j.issn.0366-6964.2022.05.016

摘要/Abstract

摘要： 旨在通过转录组测序技术筛选出共轭亚油酸(conjugated linoleic acids，CLA)影响边鸡胸肌脂类代谢的相关基因，为日粮中CLA调控边鸡脂类代谢的作用机制提供理论依据。本试验选取90日龄健康边鸡180只，随机分为5组，每组3个重复，每组日粮中分别添加不同比例的CLA 0%(对照组)、0.5%、1.0%、1.5%和2.0%，预饲期1周，正饲期6周。饲养试验结束后采集胸肌组织进行转录组测序，使用DESeq2软件对测序数据进行差异表达分析，对差异表达基因进行GO功能和KEGG通路富集分析，筛选出与胸肌脂类代谢相关的差异表达基因，利用qPCR对差异表达基因进行验证。通过转录组测序数据分析，共筛选出1 229个差异表达基因，其中594个基因上调，635个基因下调。随机选取9个差异表达基因进行qPCR验证，其相对表达量变化趋势与测序结果一致。GO功能分析发现，差异表达基因主要集中在生物学过程中的细胞过程、单一生物过程、生物调节和代谢过程。KEGG通路富集分析发现，各试验组中差异表达基因显著富集在不同的信号通路中，主要富集在ECM-受体相互作用、黏着斑、吞噬体、细胞凋亡、肌动蛋白细胞骨架调节和细胞因子-细胞因子受体相互作用等通路中。通过GO和KEGG功能注释分析，共筛选出MCAT、APOA1、PTGDS、ALDH3A2、PLTP、FABP3、FOXO1、UCP3和FABP4等18个主要的参与脂类代谢相关的差异表达基因，可能在调控边鸡胸肌脂类代谢中发挥重要的作用。本研究通过转录组测序筛选出CLA影响边鸡胸肌脂类代谢过程中的主要调控基因，发现了日粮中CLA影响差异表达基因作用的主要生物学过程和信号通路，为今后研究CLA影响边鸡胸肌脂类代谢的分子作用机制奠定了基础。

关键词: 边鸡, 共轭亚油酸, 转录组, 脂类代谢

Abstract: The study aimed to screen out the genes related to lipid metabolism in breast muscle of Bian chickens affected by conjugated linoleic acids(CLA) through transcriptome sequencing technology, and provide the theoretical basis for the mechanism of dietary CLA regulating the lipid metabolism. In this experiment, 180 90-day-old healthy Bian chickens were randomly divided into 5 gourps with 3 replicates in each group, and chickens in different groups were fed diets added different proportions CLA 0% (control group), 0.5%, 1.0%, 1.5% and 2.0%, respectively, the pre-feeding period lasted for 1 week followed by an experimental period of 6 weeks. After the feeding experiment, breast muscles were collected for transcriptome sequencing. DESeq2 software was used for differential expression analysis of the sequencing data, the differentially expressed genes were detected by GO function and KEGG pathway enrichment analysis, and differentially expressed genes related to lipid metabolism in breast muscle were screened out. the differentially expressed genes were verified by qPCR.Through analyzing transcriptome sequencing data, a total of 1 229 differentially expressed genes were screened out, 594 genes were up-regulated and 635 genes were down-regulated. The results of 9 randomly selected differentially expressed genes by qPCR verification showed that the relative expression trend was consistent with the sequencing results. GO functional analysis found that differentially expressed genes were mainly concentrated in the biological process, including cellular process, single-organism process, biological regulation and metabolic process. KEGG pathway enrichment analysis showed that the differentially expressed genes in each experimental group were significantly enriched in different signaling pathways, mainly enriched in ECM-receptor interaction, focal adhesion, phagosome, apoptosis, regulation of actin cytoskeleton and cytokine-cytokine receptor interaction. Through GO and KEGG function annotation analysis, a total of 18 main differentially expressed genes involved in lipid metabolism were screened out, including MCAT, APOA1, PTGDS, ALDH3A2, PLTP, FABP3, FOXO1, UCP3 and FABP4, etc., which may play an important role in regulating the lipid metabolism in breast muscle of Bian chickens. In this study, transcriptome sequencing was used to screen the main regulatory genes in the process of CLA affecting lipid metabolism in breast muscles of Bian chicken, and the main biological processes and signaling pathways were found, which lays the foundation for the future study of the molecular mechanism of CLA affecting the lipid metabolism in breast muscle of Bian chickens.

Key words: Bian chickens, conjugated linoleic acids, transcriptome, lipid metabolism

中图分类号:

张俊珍, 张蒙, 李亚莉, 常强强, 孙天原. 共轭亚油酸影响边鸡胸肌脂类代谢的相关差异基因的筛选[J]. 畜牧兽医学报, 2022, 53(5): 1486-1499.

ZHANG Junzhen, ZHANG Meng, LI Yali, CHANG Qiangqiang, SUN Tianyuan. Screening of Differentially Expressed Genes Related to Lipid Metabolism in Breast Muscle of Bian Chickens by Adding Conjugated Linoleic Acids in Diet[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1486-1499.

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