益生菌互作对肉鸡生长性能、肠道消化吸收及糖转运蛋白GLUT2影响的研究

doi:10.11843/j.issn.0366-6964.2020.09.014

摘要/Abstract

摘要： 旨在研究益生菌互作对肉鸡生长性能、肠道养分消化吸收及相关糖转运蛋白的影响。本研究中，200只1日龄雄性爱拔益加肉雏鸡被随机分为4组，每组5个重复，每个重复10只。将干酪乳杆菌、嗜酸乳杆菌和双歧杆菌分别按比例混合后于牛乳中发酵。对照组正常饮水（control组），益生菌Ⅰ组（混合比例为2：1：1）、Ⅱ组（混合比例为1：2：1）、Ⅲ组（混合比例为1：1：2）补充1%复合菌乳制品于饮用水中，各组皆饲喂基础日粮。整个试验饲养周期为42 d，每21 d为一个阶段。结果表明：1）与对照组相比，益生菌互作组均可显著提高肉鸡平均日增重（P<0.05），改善饲料利用率（P>0.05），在所有益生菌互作组中，益生菌Ⅰ组的肉鸡生长性能最好；2）益生菌互作显著刺激了空肠淀粉酶、脂肪酶、胰蛋白酶的特异性活性（P<0.05）；3）益生菌互作组均显著提高了小肠绒毛高度（P<0.05），降低了隐窝深度（P<0.05），上调了GLUT2 mRNA的表达（P<0.05），其中，以益生菌Ⅰ、Ⅲ组作用最佳。综上所述，益生菌互作能够增强消化酶活性、改善消化道结构、上调营养转运蛋白表达，提高养分的消化率及能量吸收有效性，从而提高肉鸡的生产性能；且当干酪乳杆菌、嗜酸乳杆菌和双歧杆菌的混合配比为2：1：1时，复合益生菌制剂对肉鸡的作用效果较佳。

关键词: 益生菌, 肠道结构, 消化吸收, GLUT2

Abstract: The aim of this study was to investigate the effects of probiotic interaction on growth performance, intestinal nutrient digestion and absorption, and related sugar transporters in broiler. Two hundred 1-day-old male Arbor Acres broilers were selected, and then randomly divided into 4 groups with 5 replicates per group and 10 for each replicate. Lactobacillus casei, Lactobacillus acidophilus and Bifidobacterium lactis were mixed in proportion, and then fermented in milk. The chicks in the control group received normal drinking water, the chicks in probiotic group Ⅰ (mixed ratio of 2:1:1), group Ⅱ (mixed ratio of 1:2:1), and group III (mixed ratio of 1:1:2) received drinking water supplemented with 1% compound probiotics. All chickens were fed with normal diet for 42 days, every 21 days as a stage. The results showed that: 1) Compared to control group, the ADG(P<0.05) and feed conversion ratio (P>0.05) of broilers in all probiotic groups were improved. Furthermore, the production performance of broilers in probiotic group Ⅰ was higher than those of probiotic group Ⅱ and Ⅲ. 2) Compared to control group, specific activities of amylase, lipase, trypsin in jejunum of probiotic groups increased significantly (P<0.05). 3) In all probiotic groups, the intestinal villus height increased significantly (P<0.05), crypt depth decreased significantly (P<0.05), and the expression of glucose transporter 2 (GLUT2), particularly in probiotic group Ⅰ and Ⅲ, was significantly upregulated (P<0.05). In conclusion, probiotics interaction can enhance digestive enzyme activity, improve the structure of digestive tract, upregulate the expression of nutrient transporter, improve the nutrient digestibility and energy absorption efficiency, and further improve production performance of broiler. When the mixing ratio of L. casei, L. acidophilus and B. lactis was 2:1:1, the compound probiotics had a better effect on broilers.

Key words: probiotics, intestinal structure, digestion and absorption, GLUT2

中图分类号:

S831.4

张利环, 张若男, 贾浩, 马悦悦, 朱芷葳, 李慧锋, 陈员玉. 益生菌互作对肉鸡生长性能、肠道消化吸收及糖转运蛋白GLUT2影响的研究[J]. 畜牧兽医学报, 2020, 51(9): 2165-2176.

ZHANG Lihuan, ZHANG Ruonan, JIA Hao, MA Yueyue, ZHU Zhiwei, LI Huifeng, CHEN Yuanyu. Effects of Probiotics Interaction on Growth Performance, Intestinal Digestion and Absorption, and Sugar Transporter GLUT2 in Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2165-2176.

参考文献 40

[1]	STANTON T B.A call for antibiotic alternatives research[J].Trends Microbiol,2013,21(3):111-113.
[2]	付丽,赵巍,李立佳,等.乳酸菌培养物对肉鸡生长性能及免疫功能的影响[J].中国畜牧兽医,2015,42(9):2337-2344.FU L,ZHAO W,LI L J,et al.Effects of lactic acid bacteria cultures on growth performance and immunity function of broilers[J]. China Animal Husbandry & Veterinary Medicine,2015,42(9):2337-2344.(in Chinese)
[3]	OHIMAIN E I,OFONGO R T S.The effect of probiotic and prebiotic feed supplementation on chicken health and gut microflora:a review[J].Int J Anim Vet Adv,2012,4(2):135-143.
[4]	SHANMUGASUNDARAM R,MARKAZI A,MORTADA M,et al.Research Note:effect of synbiotic supplementation on caecal Clostridium perfringens load in broiler chickens with different necrotic enteritis challenge models[J].Poult Sci,2020, 99(5):2452-2458.
[5]	DE CESARE A,SALA C,CASTELLANI G,et al.Effect of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome[J].PLoS One,2020,15(1):e0228338.
[6]	PEREIRA R,BORTOLUZZI C,DURRER A,et al.Performance and intestinal microbiota of chickens receiving probiotic in the feed and submitted to antibiotic therapy[J].J Anim Physiol Anim Nutr (Berl),2019,103(1):72-86.
[7]	曾娟娟,赵迪,王磊,等.干酪乳杆菌对脂多糖刺激仔猪抗氧化能力的影响[C]//第七届中国饲料营养学术研讨会论文集.郑州:中国畜牧兽医学会动物营养学分会,2014:177.ZENG J J,ZHAO D,WANG L,et al.Effect of Lactobacillus casei on the antioxidant capacity of piglets stimulated by lipopolysaccharide[C]//Proceeding of the 7th China Academic Symposium of Feed Nutrition.Zhengzhou:Animal Nutrition Branch of Chinese Animal Husbandry and Veterinary Association,2014:177.(in Chinese)
[8]	RADICIONI M,KOIRALA R,FIORE W,et al.Survival of L. casei DG® (Lactobacillus paracasei CNCMI1572) in the gastrointestinal tract of a healthy paediatric population[J].Eur J Nutr,2019,58(8):3161-3170.
[9]	郭元晟,闫素梅,史彬林,等.发酵乳酸杆菌对肉鸡小肠绒毛形态的影响[J].动物营养学报,2011,23(7):1194-1200.GUO Y S,YAN S M,SHI B L,et al.Effects of Lactobacillus fermentum supplementation on the small intestinal villus structure of broilers[J].Chinese Journal of Animal Nutrition,2011,23(7):1194-1200.(in Chinese)
[10]	IRAPORDA C,ERREA A,ROMANIN D E,et al.Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells[J]. Immunobiology, 2015, 220(10):1161-1169.
[11]	HIGGINS J P,HIGGINS S E,VICENTE J L,et al.Temporal effects of lactic acid bacteria probiotic culture on Salmonella in neonatal broilers[J].Poult Sci,2007,86(8):1662-1666.
[12]	CHUKWUMA C I,ISLAM S.Effects of xylitol on carbohydrate digesting enzymes activity,intestinal glucose absorption and muscle glucose uptake:a multi-mode study[J].Food Funct,2015,6(3):955-962.
[13]	BRÖER S.Amino acid transport across mammalian intestinal and renal epithelia[J].Physiol Rev,2008,88(1):249-286.
[14]	MUECKLER M,THORENS B.The SLC2 (GLUT) family of membrane transporters[J].Mol Aspects Med,2013,34(2-3):121-138.
[15]	SALEH A A,EL-FAR A H,ABDEL-LATIF M A,et al.Exogenous dietary enzyme formulations improve growth performance of broiler chickens fed a low-energy diet targeting the intestinal nutrient transporter genes[J].PLoS One,2018,13(5):e0198085.
[16]	MANGIAN H F,TAPPENDEN K A.Butyrate increases GLUT2 mRNA abundance by initiating transcription in Caco2-BBe cells[J].JPEN J Parenter Enteral Nutr,2009,33(6):607-617.
[17]	RUHNKE I,RÖHE I,GOODARZI BOROOJENI F,et al.Feed supplemented with organic acids does not affect starch digestibility,nor intestinal absorptive or secretory function in broiler chickens[J].J Anim Physiol Anim Nutr (Berl),2015, 99(S1):29-35.
[18]	王冬群,丁雪梅,白世平,等.不同非淀粉多糖复合酶在肉鸡玉米-豆粕型饲粮中的应用效果[J].动物营养学报, 2013, 25(10):2459-2473.WANG D Q,DING X M,BAI S P,et al.Application effects of different non-starch polysaccharide complex enzymes on corn-soybean meal diets for broilers[J].Chinese Journal of Animal Nutrition,2013,25(10):2459-2473.(in Chinese)
[19]	董佳楠,易晓菲,张维刚,等.乳酸菌和酵母菌复合培养物对肉仔鸡生长性能的影响[J].饲料工业,2019,40(6):17-22.DONG J N,YI X F,ZHANG W G,et al.Effect of compound culture of lactic acid bacteria and yeast on growth performance of broilers[J]. Feed Industry,2019,40(6):17-22.(in Chinese)
[20]	郝志敏,蔡晶晶,潘晓花,等.益生菌对快大黄羽肉鸡生产性能的影响[J].中国饲料添加剂,2011(11):22-27.HAO Z M,CAI J J,PAN X H,et al.Effects of probiotics on the production performance of fast-large-scale yellow-feathered chicken[J].China Feed Additive,2011(11):22-27.(in Chinese)
[21]	刘淑娇,张东旭,刘丽敏.复合益生菌制剂对肉仔鸡生产性能、血清生化指标和免疫功能的影响[J].中国饲料,2019(2):34-38.LIU S J,ZHANG D X,LIU L M.Effects of compound probiotics on growth performance,serum biochemical indexes and immunity function of broilers[J].China Feed,2019(2):34-38.(in Chinese)
[22]	江琳琳,姚学强,张毅峰,等.添加乳酸杆菌和链球菌培养物对肉鸡生产性能影响[J].北方牧业,2019(1):28,27.JIANG L L,YAO X Q,ZHANG Y F,et al.Effect of adding Lactobacillus and Streptococcus cultures on broiler performance[J]. Beifang Muye,2019(1):28,27.(in Chinese)
[23]	MAKLED M N,ABOUELEZZ K F M,GAD-ELKAREEM A E G,et al.Comparative influence of dietary probiotic,yoghurt,and sodium butyrate on growth performance,intestinal microbiota,blood hematology,and immune response of meat-type chickens[J]. Trop Anim Health Prod,2019,51(8):2333-2342.
[24]	LEE K W,LEE S H,LILLEHOJ H S,et al.Effects of direct-fed microbials on growth performance,gut morphometry,and immune characteristics in broiler chickens[J].Poult Sci,2010,89(2):203-216.
[25]	GONG L,WANG B K,MEI X Q,et al.Effects of three probiotic Bacillus on growth performance,digestive enzyme activities,antioxidative capacity,serum immunity,and biochemical parameters in broilers[J].Anim Sci J,2018,89(11):1561-1571.
[26]	WANG Y B,GU Q.Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers[J].Res Vet Sci, 2010,89(2):163-167.
[27]	RAJPUT I R,LI Y L,XU X,et al.Supplementary effects of Saccharomyces boulardii and Bacillus subtilis B10 on digestive enzyme activities, antioxidation capacity and blood homeostasis in broiler[J].Int J Agric Biol,2013,15:231-237.
[28]	ZHANG L,ZHANG L L,ZHAN X A,et al.Effects of dietary supplementation of probiotic,Clostridium butyricum,on growth performance,immune response,intestinal barrier function,and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88[J].J Anim Sci Biotechnol,2016,7:3.
[29]	王凯英,鲍坤,徐超,等.饲料酸化剂对水貂小肠绒毛形态、营养物质消化率和N、P环境排放的影响[J].畜牧兽医学报, 2015,46(4):665-671.WANG K Y,BAO K,XU C,et al.Effect of acidifier on intestinal villus morphology,nutrient digestibility,N,P excretion of growing mink[J].Acta Veterinaria et Zootechnica Sinica,2015,46(4):665-671.(in Chinese)
[30]	BROOM L J.Organic acids for improving intestinal health of poultry[J].World Poultry Sci J,2015,71(4):630-642.
[31]	ABDEL-MONEIM A M E,ELBAZ A M,KHIDR R E S,et al.Effect of in ovo inoculation of Bifidobacterium spp. on growth performance,thyroid activity,ileum histomorphometry,and microbial enumeration of broilers[J].Probiotics Antimicrob Prot,2019, doi:10.1007/s12602-019-09613-x.
[32]	BOGUCKA J,RIBEIRO D M,BOGUSŁAWSKA-TRYK M,et al.Microstructure of the small intestine in broiler chickens fed a diet with probiotic or synbiotic supplementation[J].J Anim Physiol Anim Nutr (Berl),2019,103(6):1785-1791.
[33]	HE T F,LONG S F,MAHFUZ S,et al.Effects of probiotics as antibiotics substitutes on growth performance,serum biochemical parameters,intestinal morphology,and barrier function of broilers[J].Animals,2019,9(11):985.
[34]	SINGH A K,TIWARI U P,BERROCOSO J D,et al.Effects of a combination of xylanase,amylase and protease,and probiotics on major nutrients including amino acids and non-starch polysaccharides utilization in broilers fed different level of fibers[J].Poult Sci,2019,98(11):5571-5581.
[35]	WU Y P,XU H,CAO X F,et al.Bacillus amyloliquefaciens ameliorates H2O2-induced oxidative damage by regulating transporters,tight junctions,and apoptosis gene expression in cell line IPEC-1[J].Probiotics Antimicrob Prot,2020,12(2):649-656.
[36]	KAMINSKI N A,WONG E A.Differential mRNA expression of nutrient transporters in male and female chickens[J].Poult Sci,2018,97(1):313-318.
[37]	AWAD W A,GHAREEB K,ZENTEK J.Mechanisms underlying the inhibitory effect of the feed contaminant deoxynivalenol on glucose absorption in broiler chickens[J].Vet J,2014,202(1):188-190.
[38]	WANG H T,YU C,HSIEH Y H,et al.Effects of albusin B (a bacteriocin) of Ruminococcus albus 7 expressed by yeast on growth performance and intestinal absorption of broiler chickens-its potential role as an alternative to feed antibiotics[J].J Sci Food Agric, 2011,91(13):2338-2343.
[39]	GILBERT E R,LI H F,EMMERSON D A,et al.Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks[J].J Nutr,2008,138(2):262-271.
[40]	HAYAT K,BODINGA B M,HAN D,et al.Effects of dietary inclusion of chromium propionate on growth performance,intestinal health,immune response and nutrient transporter gene expression in broilers[J].Sci Total Environ,2020,705:135869.