山楂叶黄酮对产蛋后期种鸡蛋壳品质的影响

doi:10.11843/j.issn.0366-6964.2021.04.016

摘要/Abstract

摘要： 山楂叶黄酮（HF）是山楂叶提取物，具有抗氧化、抗炎和降血脂的作用。本试验旨在研究日粮HF对产蛋后期种母鸡蛋壳品质的影响。选取体重相近的60周龄健康种母鸡（栖岭草鸡）270只，随机分为3组，每组6个重复，每个重复15只鸡。对照组（CON）饲喂玉米-豆粕型基础日粮（BD），低剂量组（LHF）饲喂BD+30 mg·kg^-1 HF日粮，高剂量组（HHF）饲喂BD+60 mg·kg^-1 HF日粮。试验预饲2周，正式饲喂8周，最后，采集种蛋测定蛋壳品质，采集血样测定钙和磷含量，采集壳腺组织观察组织形态学、细胞凋亡和抗氧化蛋白的变化。结果显示：1）与CON组相比，HF处理显著提高了蛋壳厚度和蛋壳强度（P<0.05），对蛋黄颜色、蛋白高度、哈氏单位和壳腺的器官指数没有显著影响（P>0.05）。2）与CON组相比，HF处理显著提高了血清钙的含量（P<0.05），显著上调了壳腺部CaBP-D28K、OPN和OC-116基因mRNA的表达水平（P<0.05），HHF组壳腺部Ca²⁺ATP酶的活性显著提高（P<0.05）。3）组织学切片分析表明，HF处理显著降低了壳腺细胞的凋亡率（P<0.05），并显著提高了壳腺部绒毛的长度和腺体的密度（P<0.05）。4）与CON组相比，HF处理组壳腺部MDA的含量显著降低（P<0.05），HHF处理显著提高了GSH-Px酶的活性（P<0.05）。5）HHF组壳腺部KEAP1、p-NFκB/NFκB和Bax/Bcl2蛋白表达水平较CON组显著降低（P<0.05）；Nrf2、HO-1和PCNA蛋白的表达量则显著提高（P<0.05）。综上，日粮中添加60 mg·kg^-1的HF通过激活Nrf2/HO-1信号通路增强壳腺的抗氧化能力，改善细胞凋亡进程和钙离子转运能力，从而提高产蛋后期种鸡的蛋壳品质。

关键词: 山楂叶黄酮, 产蛋后期, 蛋壳品质, 抗氧化

Abstract: Hawthorn-leaves flavonoids (HF), extracted from hawthorn leaves, were reported to exert antioxidant, anti-inflammatory and hypolipidemic properties. The aim of this study was to investigate the effects of dietary HF on the eggshell quality of aged breeder hens. Qiling breeder hens (60-week-old) of 270 were randomly divided into 3 treatments(6 replicates per treatment, 15 hens per replicate): basic corn-soybean diet (CON); basic corn-soybean diet supplemented with 30 mg·kg^-1 HF (LHF); basic corn-soybean diet supplemented with 60 mg·kg^-1 HF (HHF). Pre-experiment period was 2 weeks，and formal experiment lasted 8 weeks. Eggs were obtained for quality determination, blood samples were taken for determining the content of Ca and P, and shell gland tissues were collected to observe the changes of histopathology, cell apoptosis and antioxidant proteins. The results showed that: 1) In comparison with the CON group, supplemental HF significantly improved the thickness and strength of the eggshell (P<0.05), but had no significant effect on the hgt, yolk color, haugh unit and relative shell gland weight (P>0.05). 2)In comparison with the CON group, serum levels of calcium were elevated and the mRNA expressions of CaBP-D28K, OPN and OC-116 genes in the shell gland of HF treated groups was significantly up-regulated (P<0.05). In the HHF group, the activity of Ca²⁺ ATP enzyme was significantly increased in comparison with the CON group (P<0.05). 3) Histological section analysis showed, HF treatment reduced the apoptosis rate of cells in the shell gland (P<0.05), and significantly increased the length of villi in the shell gland and the density of glands (P<0.05). 4) In comparison with CON group, dietary supplementation with HF significantly reduced the content of MDA in the shell gland (P<0.05), the activity of GSH-Px was significantly increased in HHF group (P<0.05). 5) In comparison with the CON group, HHF treatment significantly down-regulated the protein expression of KEAP1, p-NFκB /NFκB and Bax/Bcl2 (P<0.05), and significantly up-regulated the protein expression of Nrf2, HO-1 and PCNA (P<0.05). In summary, 60 mg·kg^-1HF treatment group could enhance the antioxidant capacity of the shell gland by activating Nrf2/ HO-1 signaling pathway, thus reducing the rate of apoptosis and improving the ability of calcium transport, so as to improve the eggshell quality of aged breeder hens.

Key words: hawthorn-leaves flavonoids, late egg-laying period, eggshell quality, antioxidant

中图分类号:

S831.4

戴宏健, 吕增鹏, 黄振吾, 李思梦, 魏全伟, 孙涛, 石放雄. 山楂叶黄酮对产蛋后期种鸡蛋壳品质的影响[J]. 畜牧兽医学报, 2021, 52(4): 1010-1019.

DAI Hongjian, Lü Zengpeng, HUANG Zhenwu, LI Simeng, WEI Quanwei, SUN Tao, SHI Fangxiong. Effects of Hawthorn-leaves Flavonoids on the Quality of Eggshells of Breeder Hens in Late Egg-laying Period[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1010-1019.

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