外源胰岛素和能量限饲对鸡PPP1R3C表达的效应

doi:10.11843/j.issn.0366-6964.2021.09.013

摘要/Abstract

摘要： 旨在检测PPP1R3C在爱拔益加肉鸡不同组织中的表达情况，探究外源胰岛素和能量限饲对鸡体胰岛素敏感组织中PPP1R3C表达的影响。试验一，取不同发育阶段的雌性肉鸡（E14、E19、D7和D21，n=10）组织样，通过qRT-PCR技术检测PPP1R3C在不同时期胸肌中的表达；试验二，腹腔注射胰岛素（INS）或PBS，取两组注射后不同时间点（0、15、120和240 min，n=5）的D24雄性肉鸡组织样，检测PPP1R3C在肉鸡不同组织中的表达，探究外源胰岛素处理对肉鸡胰岛素敏感组织中PPP1R3C表达的影响；试验三，取D18雌性肉鸡，一组饲喂常规日粮（n=20），另一组饲喂限制30%能量的日粮（n=20），饲喂至48天屠宰取样，探究30%能量限饲对肉鸡PPP1R3C表达的影响；试验四，D7雌性肉鸡随机分为3组，对照组、15%能量限饲组和15%蛋白限饲组（n=10），分别饲养至D21屠宰取样，探究能量限饲是否具有剂量依赖性。结果表明：1）PPP1R3C在胸肌组织中高表达，其次是心和腿肌组织（P<0.05）。2）PPP1R3C表达量呈现了明显随鸡发育而升高的趋势。3） INS注射显著下调了胸肌中PPP1R3C的表达，在注射后120 min时PPP1R3C的表达显著低于0和15 min （P<0.05）；PBS注射后胸肌中PPP1R3C的表达没有显著差异，在注射后120和240 min时，INS组PPP1R3C表达显著低于PBS组（P<0.05）。INS注射也降低了肝中PPP1R3C的表达，在INS处理15 min时PPP1R3C的表达已显著低于0 min （P<0.05）；PBS注射后肝中PPP1R3C表达处于动态平衡，在注射后120 min时，INS组PPP1R3C表达显著低于PBS组（P<0.05）。胰岛素注射后在腹脂中出现了与胸肌和肝相反的结果，在胰岛素注射后15 min时PPP1R3C的表达显著高于0、120、和240 min （P<0.05），而0、120、和240 min之间没有显著差异；PBS注射后腹脂中PPP1R3C表达没有显著变化，在注射后15 min时，INS组PPP1R3C表达量显著高于PBS组（P<0.05）。4）30%能量限饲导致PPP1R3C在胸肌和肝中的表达均显著下调（P<0.05）。5）15%能量限饲和15%蛋白限饲不能显著影响胸肌中PPP1R3C的表达。以上研究表明，PPP1R3C在胸肌中表达最高，并随着个体发育表达上升，且外源胰岛素对PPP1R3C的表达效应具有明显的组织特异性。30%能量限饲可产生类似于外源胰岛素的效应，且能量限饲存在一定的剂量依赖性，本研究结果为进一步揭示鸡PPP1R3C功能奠定了基础。

关键词: PPP1R3C, 鸡, 能量限饲, 胰岛素

Abstract: The purpose of this study was to detect the expression of PPP1R3C in different tissues of AA broilers and to explore the effects of exogenous insulin and energy restriction on the expression of PPP1R3C in insulin sensitive tissues of chickens. In experiment 1, tissue samples of female broilers at different developmental stages (E14, E19, D7 and D21, n=10) were taken to detect the expression of PPP1R3C in chest muscle by qRT-PCR technique; In experiment 2, the tissue samples of D24 male broilers at different time points (0, 15, 120 and 240 min, n=5) after intraperitoneal injection of insulin or PBS were taken, to detect the expression of PPP1R3C in different tissues of broilers, and to explore the effect of exogenous insulin treatment on the expression of PPP1R3C in insulin sensitive tissues of broilers; In experiment 3, one group of D18 female broilers was fed with conventional diet (n=20) and the other group was fed diet with 30% energy restriction (n=20). After feeding for 48 days, slaughtering samples were taken to explore the effect of 30% energy restriction on the expression of PPP1R3C in broilers; In experiment 4, D7 female broilers were randomly divided into 3 groups:control group, 15% energy restriction group and 15% protein restriction group (n=10). Slaughtering samples were taken from D21 female broilers to explore whether energy restriction was dose-dependent. The results showed that:1) PPP1R3C was highly expressed in chest muscle, followed by heart and leg muscle(P<0.05). 2) The expression of PPP1R3C showed a increasing trend with the growth and development of chicken. 3) Insulin injection significantly down-regulated the expression of PPP1R3C in chest muscle, and the expression of PPP1R3C at 120 min after insulin injection was significantly lower than that at 0 and 15 min(P<0.05); There was no significant change in the expression of PPP1R3C in chest muscle after PBS injection, the expression of PPP1R3C in INS group was significantly lower than that in PBS group at 120 and 240 min after injection(P<0.05). Insulin injection also decreased the expression of PPP1R3C in liver, and the expression of PPP1R3C after insulin treatment for 15 min was significantly lower than at 0 min (P<0.05); The expression of PPP1R3C in liver was in dynamic balance after PBS injection, at 120 min after injection, the expression of PPP1R3C in INS group was significantly lower than that in PBS group(P<0.05). The expression of PPP1R3C in abdominal fat was opposite to that in chest muscle and liver after insulin injection, the expression of PPP1R3C at 15 min after insulin injection was significantly higher than that at 0,120 and 240 min(P<0.05), but there was no significant difference among 0, 120, 240 min; There was no significant change in the expression of PPP1R3C in abdominal fat after PBS injection, the expression of PPP1R3C in INS group was significantly higher than that in PBS group at 15 min after injection (P<0.05). 4) The expression of PPP1R3C in chest muscle and liver was significantly down-regulated by 30% energy restriction (P<0.05). 5) 15% energy restriction and 15% protein restriction did not significantly affect the expression of PPP1R3C in chest muscle. The study results showed that the expression of PPP1R3C was the highest in the chest muscle, and increased with the development of broilers, and the effect of exogenous insulin on the expression of PPP1R3C had obvious tissue specificity. At the same time, the results of energy restriction showed that 30% energy restriction could produce an effect similar to that of exogenous insulin, and the energy restriction had a certain dose dependence, which laid a foundation for further revealing the function of chicken PPP1R3C.

Key words: PPP1R3C, chicken, energy restriction, insulin

中图分类号:

S831.4

高林歌, 邵冰豪, 朱星浩, 陈博, 郭钰君, 黄艳群, 陈文. 外源胰岛素和能量限饲对鸡PPP1R3C表达的效应[J]. 畜牧兽医学报, 2021, 52(9): 2500-2509.

GAO Lin'ge, SHAO Binghao, ZHU Xinghao, CHEN Bo, GUO Yujun, HUANG Yanqun, CHEN Wen. Effects of Exogenous Insulin and Energy Restriction on PPP1R3C Expression in Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2500-2509.

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