芦丁对围产期湖羊瘤胃发酵、瘤胃菌群结构及抗氧化性能的影响

doi:10.11843/j.issn.0366-6964.2023.07.021

摘要/Abstract

摘要： 旨在研究饲粮中添加芦丁对围产期湖羊瘤胃发酵、瘤胃菌群结构及抗氧化性能的影响。选择30只围产期相近、体重[（62.90±2.80） kg]相似的24月龄围产期湖羊，随机分为3组，每组10个重复。芦丁在基础饲粮中的添加剂量分别为0 mg·kg^-1 BW （CON组）、50 mg·kg^-1 BW （Ⅰ组）和100 mg·kg^-1 BW （Ⅱ组）。预试期7 d，正式试验期56 d。产后28 d晨饲前，每组随机选取5只羊屠宰，检测瘤胃组织形态、瘤胃发酵参数、抗氧化性能及瘤胃菌群结构。结果发现：1）各组围产期湖羊日粗饲料采食量、日干物质采食量和日中性洗涤纤维采食量差异均不显著（P>0.05）；2个试验组围产期湖羊的产后体重、末体重（FBW）和平均日增重（ADG）均高于CON组，但差异不显著（P>0.05），各组产羔数量及羔羊体重无显著变化（P>0.05）；2）Ⅱ组围产期湖羊瘤胃乳头长度显著高于CON组（P<0.05）；3）Ⅰ组瘤胃液中谷胱甘肽过氧化酶（GSH-Px）和过氧化氢酶（CAT）活性显著高于CON组（P<0.05）；4）与CON组相比，Ⅱ组围产期湖羊瘤胃液中菌体蛋白、原虫蛋白、乙酸、丙酸、戊酸和总挥发性脂肪酸（TVFA）浓度显著升高（P<0.05），微生物蛋白浓度极显著升高（P<0.01）；5） CON组和Ⅰ组围产期湖羊瘤胃细菌第一优势菌门为厚壁菌门，第二优势菌门为拟杆菌门；Ⅱ组第一优势菌门为拟杆菌门，第二优势菌门为厚壁菌门。与CON组相比，Ⅱ组厚壁菌门和螺旋体门相对丰度均显著降低（P<0.05），拟杆菌门相对丰度显著升高（P<0.05）。各组围产期湖羊瘤胃细菌的优势菌属均为普雷沃菌属。与CON组相比，Ⅰ组普雷沃菌属相对丰度显著升高（P<0.05）；Ⅱ组普雷沃菌属相对丰度极显著升高（P<0.01），Ⅱ组密螺旋体属相对丰度显著降低（P<0.05）。综上表明，芦丁对围产期湖羊瘤胃组织形态有改善作用，能够提高瘤胃抗氧化能力，改变瘤胃菌群结构，有效改善围产期湖羊瘤胃发酵功能。

关键词: 芦丁, 围产期湖羊, 瘤胃组织, 抗氧化性能, 瘤胃发酵, 瘤胃菌群结构

Abstract: The aim of this experiment was to study the effect of rutin addition to the diet on rumen fermentation, rumen flora structure and antioxidant properties in perinatal Hu sheep. Thirty 24-month-old perinatal Hu sheep with similar perinatal period and similar body weight[(62.90±2.80) kg] were selected and randomly divided into three groups with 10 replicates in each group. The rutin additive dose was 0 mg·kg^-1 BW (Control group), 50 mg·kg^-1 BW (Group Ⅰ) and 100 mg·kg^-1 BW (Group II) to the basal diet, respectively. The pre-test period was 7 d and the formal test period was 56 d. At 28 days postpartum before morning feeding, five sheep in each group were randomly selected for slaughter, and rumen tissue morphology, rumen fermentation parameters, antioxidant properties and rumen flora structure were examined. The results showed that:1) The differences in daily roughage intake, daily dry matter intake and daily neutral detergent fiber intake of perinatal Hu sheep were not significant (P>0.05) among the groups; Postpartum body weight, FBW and ADG were higher in the 2 experimental groups of perinatal Hu sheep than in the Control group, but the differences were not significant (P>0.05), there was no significant change in the number of lambing and the weight of lambs in each group (P>0.05); 2) The rumen papilla length of perinatal Hu sheep in Group II was significantly increased compared with that in Control group (P<0.05); 3) GSH-Px and CAT activities in rumen fluid of Group I were significantly higher than those in Control group (P<0.05); 4) Compared with the Control Group, the concentration of bacterial protein, protozoan protein, acetic acid, propionic acid, valeric acid and total volatile fatty acids (TVFA) in the rumen fluid of perinatal Hu sheep in Group II were significantly higher (P<0.05), and the concentration of microbial protein was extremely significantly higher (P<0.01); 5) The first dominant bacterial phylum in the rumen of perinatal Hu sheep in the Control group and Group I was the Frimicutes, and the second dominant bacterial phylum was the Bacteroidetes; The first dominant bacterial phylum in the Group II was the Bacteroidetes, and the second bacterial dominant phylum was the Frimicutes. Compared with the Control group, the relative abundance of both the Frimicutes and the Spirochaetes were significantly lower in Group II (P<0.05), and the relative abundance of the Bacteroidetes was significantly higher in Group II (P<0.05). The dominant genus of rumen bacteria in all groups of perinatal Hu sheep was Prevotella. Compared with the Control group, the relative abundance of Prevotella was significantly higher in Group I (P<0.05); The relative abundance of Prevotella was highly significantly higher in Group II (P<0.01), and the relative abundance of Treponema was significantly lower in Group II (P<0.05). In summary, rutin has improved the morphology of rumen tissue in perinatal Hu sheep, and can improve the antioxidant capacity of rumen, change the structure of rumen flora, and effectively improve the fermentation function of rumen in perinatal Hu sheep.

Key words: rutin, perinatal Hu sheep, rumen tissue, antioxidant performance, rumen fermentation, rumen flora structure

中图分类号:

S826.4

熊程坤, 张道亮, 杨悦, 丁红研, 赵杰, 李玉, 王希春, 冯士彬, 赵畅, 汤继顺, 吴金节. 芦丁对围产期湖羊瘤胃发酵、瘤胃菌群结构及抗氧化性能的影响[J]. 畜牧兽医学报, 2023, 54(7): 2898-2909.

XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.

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