畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展

doi:10.11843/j.issn.0366-6964.2024.12.001

Abstract

Abstract:

Muscle cells (also known as muscle fibers) are the basic unit of skeletal muscle, accounting for about 50% of the body of livestock and poultry, and are closely related to meat production performance and bone protection of livestock and poultry. They play an important role in regulating the metabolism and endocrine of livestock and poultry, and also provide power for the movement of livestock and poultry. Skeletal muscle is a relatively complex tissue, and the basic unit of these tissues are muscle cells. Skeletal muscle requires a variety of types of mononuclear and multinucleated cells to interact during development, which is the key to the formation of high-quality meat. In recent years, with the rapid development of science and technology, there are more and more comprehensive techniques for identifying skeletal muscle development-related cells, and single cell sequencing technology is most widely used. Its application provides technical support for the study of cell types and related cell development heterogeneity of skeletal muscle, and is of great significance for the study of meat quality. This paper reviews the identification of cells related to skeletal muscle development by cell morphology observation, molecular markers, immunohistochemistry, immunofluorescence, in situ hybridization, and single cell sequencing, and summarizes the marker genes of cells related to skeletal muscle development, in order to understand the structure and function of skeletal muscle. It provides a reference for exploring the mechanism of skeletal muscle development, provides a theoretical basis for the study of meat quality, and also has important significance and practical value in individual breeding of different varieties.

Key words: skeletal muscle, skeletal muscle development, cell identification

CLC Number:

S852.2

WU Danni, XIE Yuchun, QIN Qing, ZHANG Chongyan, XU Xiaolong, ZHAO Dan, LAN Mingxi, YANG Ji, XU Songsong, LIU Zhihong. Research Progress on Cell Types and Identification Methods Related to Muscle Fiber Development in Livestock and Poultry[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5325-5339.

Figures/Tables 5

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动物 Animal	肌肉部位 Muscle types	时期 Period	不同发育时期细胞类型变化情况 Changes of cell types at different developmental stages	采用的技术 Used technology	主要细胞类型 Main cell types	参考文献 Reference
牛 Cattle	背最长肌	胚胎期(怀孕后60 d)、哺乳期(出生后4月龄)和成年期(出生后24月龄)	胚胎期成纤维细胞占比最多；哺乳期内皮细胞占比最多，其次是IIX型肌纤维；成年期IIX型肌纤维占比最多	10×Genomics单细胞RNA测序	18个主要细胞类型：脂肪细胞、内皮细胞、红系细胞、成纤维细胞、淋巴细胞、单核细胞、成肌细胞、肌细胞、肌源性祖细胞、中性粒细胞、周细胞、雪旺氏细胞、腱细胞、Ⅰ型纤维1、Ⅰ型纤维2、IIA型纤维1、IA型纤维2和IIX型纤维	[102]
猪 Pig	背最长肌	1日龄新生猪	/	10×Genomics单细胞RNA测序	11种不同的细胞类群，包括内皮细胞(24.39%)、肌管(18.82%)、纤维成脂祖细胞(18.11%)、卫星细胞(16.74%)、成肌细胞(3.99%)、肌细胞(5.74%)、雪旺细胞(3.81%)、平滑肌细胞(3.22%)、树突状细胞(2.99%)、周细胞(1.86%)和中性粒细胞(0.33%)	[66]
鸡 Chicken	胸肌	生长早期(孵化后第5天)和快速生长期(第100天)	在生长早期鉴定到了5个成肌细胞群和2个脂肪细胞群，在快速生长期鉴定到了1个成肌细胞群和脂肪细胞群	10×Genomics单细胞RNA测序	生长早期(孵化后第5天)鉴定出4种细胞类型：成肌细胞、脂肪细胞、红细胞和其他细胞。快速生长期(第100天)鉴定出6种细胞类型：成肌细胞、脂肪细胞、干细胞、红细胞、内皮细胞和其他细胞	[103]

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Research Progress on Cell Types and Identification Methods Related to Muscle Fiber Development in Livestock and Poultry

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