单细胞转录组测序技术在家养动物中的应用

doi:10.11843/j.issn.0366-6964.2024.08.002

Abstract

Abstract:

Single-cell transcriptome sequencing technology(scRNA-seq) can identify individual cell types and cell states with high-precision resolution, breaking the dilemma that ordinary transcriptome sequencing unable to probe the specific expression characteristics of target cells, and playing an important role in the exploration of various fields, which is now widely used in many fields, such as human and mouse developmental biology, oncology, immunology, complex diseases, intestinal microbiome and clinical applications. In recent years, scRNA-seq has also carried out some pioneering research in the field of animal husbandry, mainly focusing on animal reproductive performance, embryonic development, analysis of key traits, etc. However, the application of scRNA-seq in other fields remains to be in-depth compared to its application in humans. In this paper, workflow of scRNA-seq and its application in domesticated animals is reviewed, intending to provide a reference for improving the efficiency of scRNA-seq analysis and its innovative application in domesticated animals.

Key words: single-cell, transcriptome sequencing, domesticated animals

CLC Number:

S813.1

Xiaoxu ZHANG, Hao LI, Pingjie FENG, Hao YANG, Xinyue LI, Ran LÜ, Zhangyuan PAN, Mingxing CHU. Application of Single-Cell Transcriptome Sequencing Technology in Domesticated Animals[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3276-3287.

Figures/Tables 1

References 85

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技术/工具 Technology/tools	应用 Application	参考文献 References
scRNA-seq+细胞示踪技术 scRNA-seq+cellular tracer technology	肝细胞移植后状态的变化和分区特征研究	[77]
RIBOmap	小鼠脑组织中细胞类型特异性和脑区特异性翻译调控研究	[78]
scRNA-seq+成像质谱分析scRNA-seq+IMS	活化的恒定自然杀伤T细胞在胰腺癌肝转移中的保护功能研究	[79]
autoCell	高维稀疏scRNA-seq数据集的分布推断	[4]
scGCL	scRNA-seq数据估算	[82]
MCGL	scRNA-seq数据集的细胞聚类性能提高	[83]
CAME	跨物种细胞类型分配和基因模块提取	[7]
scRNA-tools	分析和处理scRNA-seq数据	[84]

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Application of Single-Cell Transcriptome Sequencing Technology in Domesticated Animals

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