Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (9): 4593-4603.doi: 10.11843/j.issn.0366-6964.2025.09.037
• Preventive Veterinary Medicine • Previous Articles Next Articles
WANG Ziyi1,2,3(
), RU Yi1,2, LU Bingzhou1,2, YANG Yang1,2, ZHAO Longhe1,2, LI Yajun1,2, LI Jianbin1,2, LI Minggui1,2, MA Kun1,2, LENG Feifan3, HAO Rongzeng1,2,*(), ZHENG Haixue1,2,3,*()
Received:2024-12-12
Online:2025-09-23
Published:2025-09-30
Contact:
HAO Rongzeng, ZHENG Haixue
E-mail:215391193@qq.com;haorongzeng@163.com;zhenghaixue@caas.cn
CLC Number:
WANG Ziyi, RU Yi, LU Bingzhou, YANG Yang, ZHAO Longhe, LI Yajun, LI Jianbin, LI Minggui, MA Kun, LENG Feifan, HAO Rongzeng, ZHENG Haixue. Prolyl Oligopeptidase Promotes the Replication of Foot and Mouth Disease Virus in PK15 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4593-4603.
Table 1
Sequences of primer and siRNA used in this study"
| 引物名称 Primer names | 正向序列(5′→3′) Sense | 反向序列(5′→3′) Antisense |
| POP | GAATTCTACCCATACGATGTTCCAGATTACGCT CTGTCCTTCCAGTACCCCGA | GGTACCACATCGACTGGATTCCGTAA |
| FMDV-SYBR | TGGGACCATACAGGAGAAGT | GTAGCTTGGAATCTCGAAGAGG |
| SusGAPDH | ACTGAGGACCAGGTTGTGT | AGGAAATGAGCTTGACGAAGTG |
| POPsiRNA-112 | GGAUUAUCAUGGACAUAAATT | UUUAUGUCCAUGAUAAUCCTT |
| POPsiRNA-563 | CUUGAAAGAGUCAAGUUCATT | UGAACUUGACUCUUUCAAGTT |
| POPsiRNA-1404 | CAAAGAUCCCCAUGUUCAUTT | AUGAACAUGGGGAUCUUUGTT |
| NC-siRNA | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Fig. 1
Impact of FMDV infection on POP expression in PK15 Cells A. Detection of POP protein expression in PK15 cells after FMDV infection by Western blot; B. Quantification of POP mRNA expression in PK15 cells after FMDV infection using RT-qPCR; C. Quantification of FMDV mRNA level in PK15 cells after FMDV infection using RT-qPCR. ns. P>0.05; **. P < 0.01; ***. P < 0.001"
Fig. 2
Identification of POP expressing post transfected in PK15 cells A. Detection of POP protein expression in PK15 cells after transfection by Western blot; B. Quantification of POP mRNA expression in PK15 cells using RT-qPCR. ***. P < 0.001"
Fig. 3
Enhancement of FMDV replication in PK15 cells by overexpression of POP protein A. Dose-dependent effect of POP overexpression on the expression level of FMDV VP1 protein detected by Western blot; B. Detection of viral titers infected with FMDV after POP protein overexpression in PK15 cells; C. Quantification of FMDV mRNA level after overexpression of POP protein in PK15 cells using RT-qPCR. ns. P>0.05; *.P < 0.05; ***. P < 0.001"
Fig. 4
The effect of POP overexpression on FMDV replication at different time points A. Detection of FMDV VP1 protein expression levels by Western blot after POP overexpression in PK15 cells; B. Detection of viral titers infected with FMDV after POP protein overexpression in PK15 cells in different timepoint; C. Quantification of FMDV mRNA level after overexpression of POP protein in PK15 cells using RT-qPCR. ns. P>0.05; ***. P < 0.001"
Fig. 5
Screening for siRNA targeting POP gene A. Detection of POP protein expression by Western blot after transfected siRNA in PK15 cells; B. Quantification of POP mRNA level after transfected siRNA in PK15 cells using RT-qPCR. ns. P>0.05; *. P < 0.05; **. P < 0.01; ***. P < 0.001"
Fig. 6
Effect of interfering with POP endogenous expression on the replication of FMDV in PK15 cells A. Detection of FMDV VP1 protein expression by Western blot after transfected siRNA in PK15 cells; B. Detection of viral titers after POP knockdown expression in PK15 cells; C. Quantification of FMDV mRNA level after transfected siRNA in PK15 cells using RT-qPCR. ***. P < 0.001"
| 1 | 刘湘涛,张强,郭建宏.口蹄疫[M].北京:中国农业出版社,2015. |
| LIUX T,ZHANGQ,GUOJ H.Foot-and-mouth disease[M].Beijing:China Agriculture Press,2015. | |
| 2 |
JAMALS M,BELSHAMG J.Foot-and-mouth disease: past, present and future[J].Vet Res,2013,44(1):116.
doi: 10.1186/1297-9716-44-116 |
| 3 |
ALEXANDERSENS,KITCHINGR P,MANSLEYL M,et al.Clinical and laboratory investigations of five outbreaks of foot-and-mouth disease during the 2001 epidemic in the United Kingdom[J].Vet Rec,2003,152(16):489-496.
doi: 10.1136/vr.152.16.489 |
| 4 |
PERRYB D,RICHK M.Poverty impacts of foot-and-mouth disease and the poverty reduction implications of its control[J].Vet Rec,2007,160(7):238-241.
doi: 10.1136/vr.160.7.238 |
| 5 |
KNIGHT-JONEST J,RUSHTONJ.The economic impacts of foot and mouth disease-what are they, how big are they and where do they occur?[J].Prev Vet Med,2013,112(3-4):161-173.
doi: 10.1016/j.prevetmed.2013.07.013 |
| 6 | WOAH. Foot and mouth disease[Z/OL]. 2024. [2025-07-02] https://www.woah.org/en/disease/foot-and-mouth-disease/. |
| 7 |
LIK,WANGC,YANGF,et al.Virus-host interactions in foot-and-mouth disease virus infection[J].Front Immunol,2021,12,571509.
doi: 10.3389/fimmu.2021.571509 |
| 8 | LOGANG,NEWMANJ,WRIGHTC F,et al.Deep Sequencing of foot-and-mouth disease virus reveals RNA sequences involved in genome packaging[J].J Virol,2017,92(1):e01159-17. |
| 9 |
VILLALON-LETELIERF,BROOKSA G,SAUNDERSP M,et al.Host cell restriction factors that limit influenza A infection[J].Viruses,2017,9(12):376.
doi: 10.3390/v9120376 |
| 10 |
WALTERR,SHLANKH,GLASSJ D,et al.Leucylglycinamide released from oxytocin by human uterine enzyme[J].Science,1971,173(3999):827-829.
doi: 10.1126/science.173.3999.827 |
| 11 |
TARASZKIEWICZA,SINKIEWICZI,SOMMERA,et al.The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins[J].Crit Rev Food Sci Nutr,2024,64(19):6567-6580.
doi: 10.1080/10408398.2023.2170973 |
| 12 |
DUNAEVSKYY E,TERESHCHENKOVAV F,OPPERTB,et al.Human proline specific peptidases: A comprehensive analysis[J].Biochim Biophys Acta Gen Subj,2020,1864(9):129636.
doi: 10.1016/j.bbagen.2020.129636 |
| 13 | GOOSSENSF,DE MEESTERI,VANHOOFG,et al.Distribution of prolyl oligopeptidase in human peripheral tissues and body fluids[J].Eur J Clin Chem Clin Biochem,1996,34(1):17-22. |
| 14 | READ,HAZELLC,ANDREWSN W,et al.Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei[J].Acta Crystallogr Sect F Struct Biol Cryst Commun,2006,62(Pt 8):808-810. |
| 15 |
FÜLÖPV,BÖCSKEIZ,POLGÁRL.Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis[J].Cell,1998,94(2):161-170.
doi: 10.1016/S0092-8674(00)81416-6 |
| 16 |
FÜLÖPV,SZELTNERZ,POLGÁRL.Catalysis of serine oligopeptidases is controlled by a gating filter mechanism[J].EMBO Rep,2000,1(3):277-281.
doi: 10.1093/embo-reports/kvd048 |
| 17 |
SZELTNERZ,POLGÁRL.Structure, function and biological relevance of prolyl oligopeptidase[J].Curr Protein Pept Sci,2008,9(1):96-107.
doi: 10.2174/138920308783565723 |
| 18 |
SVARCBAHSR,JULKUU,KILPELAINENT,et al.New tricks of prolyl oligopeptidase inhibitors-A common drug therapy for several neurodegenerative diseases[J].Biochem Pharmacol,2019,161,113-120.
doi: 10.1016/j.bcp.2019.01.013 |
| 19 |
GRUBMANM J,BAXTB.Foot-and-mouth disease[J].Clin Microbiol Rev,2004,17(2):465-493.
doi: 10.1128/CMR.17.2.465-493.2004 |
| 20 |
WANGQ,ZHANGQ,ZHENGM,et al.Viral-host interactome analysis reveals chicken STAU2 interacts with non-structural protein 1 and promotes the replication of H5N1 avian influenza virus[J].Front Immunol,2021,12,590679.
doi: 10.3389/fimmu.2021.590679 |
| 21 |
MAS,SHIS,XUB,et al.Host serine protease ACOT2 assists DENV proliferation by hydrolyzing viral polyproteins[J].mSystems,2024,9(1):e0097323.
doi: 10.1128/msystems.00973-23 |
| 22 |
ZHANGH,WANGX,QUM,et al.Foot-and-mouth disease virus structural protein VP3 interacts with HDAC8 and promotes its autophagic degradation to facilitate viral replication[J].Autophagy,2023,19(11):2869-2883.
doi: 10.1080/15548627.2023.2233847 |
| 23 | 曾宗波,马旭升,罗志宽,等.程序性细胞死亡因子10抑制Ⅰ型干扰素表达并促进FMDV复制[J].畜牧兽医学报,2021,52(2):450-459. |
| ZENGJ B,MAX S,LUOZ K,et al.Programmed cell death factor 10 inhibits type Ⅰ interferon expression and promotes the replication of foot-and-mouth disease virus[J].Acta Veterinaria et Zootechnica Sinica,2021,52(2):450-459. | |
| 24 | ZHUZ,LIW,ZHANGX,et al.Foot-and-mouth disease virus capsid protein VP1 interacts with host ribosomal protein SA to maintain activation of the MAPK signal pathway and promote virus replication[J].J Virol,2020,94(3):e01350-19. |
| 25 |
JIANGD X,ZHANGJ B,LIM T,et al.Prolyl endopeptidase gene disruption attenuates high fat diet-induced nonalcoholic fatty liver disease in mice by improving hepatic steatosis and inflammation[J].Ann Transl Med,2020,8(5):218.
doi: 10.21037/atm.2020.01.14 |
| 26 |
HARWOODA J.Prolyl oligopeptidase, inositol phosphate signalling and lithium sensitivity[J].CNS Neurol Disord Drug Targets,2011,10(3):333-339.
doi: 10.2174/187152711794653779 |
| 27 |
SCHULZI,GERHARTZB,NEUBAUERA,et al.Modulation of inositol 1, 4, 5-triphosphate concentration by prolyl endopeptidase inhibition[J].Eur J Biochem,2002,269(23):5813-5820.
doi: 10.1046/j.1432-1033.2002.03297.x |
| 28 |
LINS Z,WUW J,CHENGY Q,et al.Prolyl endopeptidase remodels macrophage function as a novel transcriptional coregulator and inhibits fibrosis[J].Exp Mol Med,2023,55(7):1437-1450.
doi: 10.1038/s12276-023-01027-8 |
| 29 |
SAKAGUCHIM,MATSUDAT,MATSUMURAE,et al.Prolyl oligopeptidase participates in cell cycle progression in a human neuroblastoma cell line[J].Biochem Biophys Res Commun,2011,409(4):693-698.
doi: 10.1016/j.bbrc.2011.05.066 |
| 30 |
SUZUKIK,SAKAGUCHIM,TANAKAS,et al.Prolyl oligopeptidase inhibition-induced growth arrest of human gastric cancer cells[J].Biochem Biophys Res Commun,2014,443(1):91-96.
doi: 10.1016/j.bbrc.2013.11.051 |
| 31 |
TANAKAM,DOHGUS,KOMABAYASHIG,et al.Brain-transportable dipeptides across the blood-brain barrier in mice[J].Sci Rep,2019,9(1):5769.
doi: 10.1038/s41598-019-42099-9 |
| 32 |
SVARCBAHSR,JANTTIM,KILPELAINENT,et al.Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A[J].Pharmacol Res,2020,151,104558.
doi: 10.1016/j.phrs.2019.104558 |
| 33 | 王姿逸,茹毅,卢炳州,等.脯氨酰寡肽酶的研究进展[J].中国兽医科学,2025,55(2):240-246. |
| WANGJ Y,RUY,LUB Z,et al.Research progress of prolyl endopeptidase[J].Chinese Veterinary Science,2025,55(2):240-246. |
| [1] | Huancheng LIAO, Zhengwang SHI, Juncong LUO, Wanying WANG, Lu FENG, Jing ZHOU, Fan ZHANG, Xintai SHI, Hong TIAN. Preparation of Monoclonal Antibody against Cathay Topotype of FMDV Type O and Development of Double Antibody Sandwich ELISA for Cathay Topotype of FMDV Type O [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4012-4020. |
| [2] | Mengdi WANG, Yumin WANG, Zhen ZHANG, Xiuxiang LU, Heng WANG, Wenjie FAN, Chen YAO, Pengxiang LIU, Yanjie MA, Beibei CHU, Jiang WANG, Guoyu YANG. Effect of TSG101 Gene Knockdown on Proliferation of Pseudorabies Virus in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4110-4120. |
| [3] | WANG Jiali, YANG Fan, SHAO Wenhua, HUANG Mengyao, CAO Weijun, PU Xiuying, ZHANG Wei, ZHENG Haixue. Construction of Tollip Knockout Pig Kidney Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1810-1818. |
| [4] | ZHANG Zixuan, ZHANG Ying, LI Zhijun, YANG Jingling, JIANG Zihao, HUANG Huamin, QI Xuefeng. The Effects of Bovine Viral Diarrhoea Virus (BVDV)-induced Ferroptosis on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5716-5724. |
| [5] | LIU Weijiao, HE Qing, JIANG Yifan, CAO Siyu, ZHANG Shirui, LÜ Jialu, LONG Ping, YANG Lingchen, ZHOU Chuan, WANG Naidong. The Role of Porcine Lck Protein in PCV2 Replication in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5751-5761. |
| [6] | Kaiheng DONG, Shulun HUANG, Fengjuan LI, Kun LI, Guo LIU, Qiang ZHANG, Huifang BAO, Hongxuan LI, Zengjun LU, Xiaoli ZHANG. Screening and Identification of the Antigenic Epitope of Pig Neutralizing Antibodies against Foot-and-mouth Disease Virus Serotype Asia 1 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5211-5221. |
| [7] | Pinghua LI, Shulun HUANG, Keqiang ZHANG, Feng LIU, Pu SUN, Dong LI, Huifang BAO, Yimei CAO, Xingwen BAI, Xueqing MA, Kun LI, Hong YUAN, Zaixin LIU, Zengjun LU. Rescue and Immunogenicity Analysis of Recombinant Foot-and-Mouth Disease Virus with the Chimera of G-H Loop Gene of the Epidemic Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5222-5229. |
| [8] | SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270. |
| [9] | ZHOU Guangqing, LIU Xiaoqing, SHI Xijuan, YANG Dapeng, YUAN Ligang, CHANG Huiyun. Establishment of a Rapid Detection SA-ELISA Method for Anti Foot-and-Mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2020-2029. |
| [10] | FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207. |
| [11] | LI Shuo, ZHANG Yun, BAI Manyuan, ZHAO Ruichong, SONG Hetao, MU Suyu, TENG Zhidong, DONG Hu, MA E'ning, SUN Shiqi, GUO Huichen, YIN Shuanghui. Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1598-1607. |
| [12] | GUO Zijing, CHEN Fei, ZHANG Zhixiong, BAI Ling, ZHANG Zhidong, LI Yanmin. Effects of Interleukin-10 on T Cell Proliferation and Its Expression of TNF-α, IFN-γ and IL-2 in Mice Infected with Foot-and-Mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 694-705. |
| [13] | CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361. |
| [14] | HAN Weijian, ZHANG Junjuan, ZHANG Yiming, WANG Jiaxin, LI Limin. Cytokine Responses of Bone Marrow-derived Mast Cells to FMDV-VLPs via Mannose Receptors [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3917-3926. |
| [15] | DUAN Zhiqiang, XIE Lingling, CHEN Jiaqi, TANG Hong, WANG Yanbi, ZHAO Caiqin, ZHAO Jiafu. Research Progress on the Interactions of Newcastle Disease Virus M Protein with Host Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 32-42. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
