Fig. 1

Identification of recombinant plasmid pPG-T7g10-S1 a. PCR identification results of pPG-T7g10-S1 plasmid (1. PCR products of the S1 gene; 2. Empty vector; 3.Negative control); b. Double enzyme digestion results of pPG-T7g10-S1 plasmid (1. Double digestion using SacⅠ and ApaⅠ enzymes; 2. Empty vector)"

Fig. 2

Identification of PCR and Western blot of recombinant bacterium pPG-T7g10-S1/L. reuteri J31 a. Identification of PCR of recombinant bacterium pPG-T7g10-S1/L. reuteri J 31 (1. pPG-T7g10-S1/L.reuteri J 31; 2. pPG-T7g10-PPT/L. reuteri J 31); b. Identification of Western blot of recombinant bacterium pPG-T7g10-S1/L. reuteri J 31 (1. Precipitation of recombinant bacterium pPG-T7g10-S1/L.reuteri J 31; 2. pPG-T7g10-PPT/L. reuteri J 31 cell precipitation)"

Fig. 3

Identification of IFA of S1 protein expressed in recombinant bacteria"

Fig. 4

Analysis of growth characteristics and genetic stability of recombinant bacteria a. The determination of growth curve; b. Analysis of genetic stability of recombinant plasmid"

Table 1

Adhesion rates Lactobacilli to IPEC-J2 cells (${\bar x}$±s, n=3)"

Fig. 5

Colonization ability of recombinant Limosilactobacillus reuteri in pig jejunal a. The number of recombinant Limosilactobacillus reuteri in the jejunal mucosa samples; b. Recombinant Limosilactobacillus reuteri copy number in the jejunal mucosa. Different letters indicate significant differences (P < 0.05) at the same time point"

Fig. 6

Detection of anti-PEDV mucosa-specific SIgA antibody levels in immunized piglets Different letters indicate significant differences (P < 0.05) at the same time point"

Fig. 7

Detection of anti-PEDV serum-specific IgG antibody levels in immunized piglets Different letters indicate significant differences (P < 0.05) at the same time point"

Table 2

The neutralizing antibody titers of sera and intestinal mucus from immunized piglets"

Fig. 8

Gene transcription levels of cytokines in serum and intestinal mucus of immunized piglets Different letters indicate significant differences (P < 0.05) at the same time point"