一株羊冠状病毒的分离鉴定及全基因组序列分析

doi:10.11843/j.issn.0366-6964.2025.09.038

Abstract

Abstract:

Coronavirus is one of the most important pathogen causing respiratory and digestive diseases in humans and animals. In order to enrich the epidemiological data of coronaviruses in China, we conducted this study on the isolation of caprine coronavirus (cpCoV) from diarrheal goat samples in Xinjiang, and further identified the biological characteristics systemly. Rectal swab samples of goats suffering diarrhea from Xinjiang were tested positive by RT-PCR for epidemiological investigation, and the positive samples were treated and inoculated with HRT-18G cells. A novel cpCoV, named as cpCoV/XJCJ2301G, was isolated and identified with obvious cytopathogenic effect. The virus was detected positively by IFA, the purified virus was observed as the diameter of 100 nm with a crown-like surrounding by transmission electron microscopy. According to the genetic evolution analysis, the XJCJ2301G strain was belong to Embecovirus of β-CoVs, which formed an independent evolutionary cluster together with the present cpCoVs. The NS4a and NS4b gene sequences of XJCJ2301G strain have a 75 nt deletion, resulting in truncation or elongation of non-structural proteins. Through sequence alignment analysis of the S gene, it was found that the differences between cpCoV and BCoV mainly present in the CTD region. CpCoV was isolated from Xinjiang for the first time, and this strain enriched the epidemiological data of animal coronavirus. Notably, there was a stepwise pattern deletion between NS4a and NS4b among bovine coronavirus, caprine coronavirus, cpCoV/XJCJ2301G and rabbit coronavirus. Strain XJCJ2301G should be a key node and play a vital role in the evolution of herbivore coronaviruses.

Key words: caprine coronavirus, isolation and identification, whole genome sequencing, evolutionary analysis

CLC Number:

S852.659.6

YU Qiurong, CAI Xuhang, HE Yi, LI Jizong, MAO Li, XU Xingang, LI Bin. Identification and Isolation of a Caprine Coronavirus and Analysis of the Complete Genome Sequence[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4604-4614.

Figures/Tables 8

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引物名称 Primer name	引物序列(5′→3′) Primer sequence	扩增产物大小/bp Product size
cpCoV-S1-F	TGCTGTTATAGGAGATTTAAAGT	1 403
cpCoV-S1-R	GCTTTAAAACAATGTTGTGCATA	1 403
cpCoV-S2-F	GCTGCTAATGTTTCTGTTAGC	1 517
cpCoV-S2-R	TCCAGTGCAATTATTATAAGCC	1 517
cpCoV-S3-F	GATCTGCTATAGAGGATTTAC	1 330
cpCoV-S3-R	TAATTACTAACTCCTGGTGTC	1 330
cpCoV-NS4ab-F	TCTTCATATGCTGTTGTACAGG	1 038
cpCoV-NS4ab-R	TCCACATCAAGAACTGGTGGT	1 038

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Identification and Isolation of a Caprine Coronavirus and Analysis of the Complete Genome Sequence

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