Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (9): 4110-4120.doi: 10.11843/j.issn.0366-6964.2024.09.035
• Basic Veterinary Medicine • Previous Articles Next Articles
Mengdi WANG2(), Yumin WANG1(
), Zhen ZHANG2, Xiuxiang LU1, Heng WANG1, Wenjie FAN1, Chen YAO1, Pengxiang LIU1, Yanjie MA1, Beibei CHU1, Jiang WANG1,*(
), Guoyu YANG1,2,*(
)
Received:
2023-12-12
Online:
2024-09-23
Published:
2024-09-27
Contact:
Jiang WANG, Guoyu YANG
E-mail:mengdi.ok@163.com;2622364663@qq.com;wangjiang@henau.edu.cn;haubiochem@163.com
CLC Number:
Mengdi WANG, Yumin WANG, Zhen ZHANG, Xiuxiang LU, Heng WANG, Wenjie FAN, Chen YAO, Pengxiang LIU, Yanjie MA, Beibei CHU, Jiang WANG, Guoyu YANG. Effect of TSG101 Gene Knockdown on Proliferation of Pseudorabies Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4110-4120.
Table 1
Primer information"
基因 Genes | 引物序列(5′→3′) Primer sequence | 序列大小/bp Sequence size | 用途 Purpose |
shRNA1 | F: CCGGGGAACAATTCCTGTGCCTTATCTCGAGATAAGGCACAGGAATTGTTCCTTTTTG R: AATTCAAAAAGGAACAATTCCTGTGCCTTATCTCGAGATAAGGCACAGGAATTGTTCC | 基因敲低 | |
shRNA2 | F: CCGGGCTGGACACATACCCATATAACTCGAGTTATATGGGTATGTGTCCAGCTTTTTG R: AATTCAAAAAGCTGGACACATACCCATATAACTCGAGTTATATGGGTATGTGTCCAGC | 基因敲低 | |
shRNA3 | F: CCGGGCAACAGGGCCACCAAATACTCTCGAGAGTATTTGGTGGCCCTGTTGCTTTTTG R: AATTCAAAAAGGAACAATTCCTGTGCCTTATCTCGAGATAAGGCACAGGAATTGTTCC | 基因敲低 | |
TSG101 | F: CAGGGCCACCAAATACTTCCT R: GGAGGGTATCCGGATGGGTA | 实时荧光定量 PCR | |
β-actin | F: CTGAACCCCAAAGCCAACCGT R: TTCTCCTTGATGTCCCGCACG | 317 | 实时荧光定量 PCR |
PRV gB | F: GGCATCGCCAACTTCTTCC R: CCTCGTCCACGTCGTCCTC | 220 | 实时荧光定量 PCR |
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