助产对奶牛产后子宫菌群的影响

doi:10.11843/j.issn.0366-6964.2022.12.035

Abstract

Abstract: Dystocia is a common clinical disease of dairy cows, and assisted calving is a common method to deal with it. The assisted calving can lead to the reduction of conception rate, the increase of frozen sperm use, the increase of empty pregnancy days and the delay of the first mating time. This study aims to analyze the effect of assisted calving on postpartum uterine microbiota of dairy cow, and to provide data reference for improving the conception rate in pasture. In a large dairy farm in Ningxia, a total of 7 samples of lavage fluid were collected from both unassisted and assisted calving cows at 45 days after delivery. Bacteria were isolated, cultured, and sequenced by 16S rRNA sequencing. A total of 10 bacterial genera and 12 kinds of bacteria were isolated, among which 5 genera of bacteria including Streptomyces, Streptococcus, Enterobacter, Staphylococcus and Aerococcus were isolated from the uterine washing fluid of non-assisted calving cows. Nine genera of bacteria such as Shigella, Acinetobacter, Pseudomonas, Corynebacterium, Bacillus, Aerococcus, Enterobacter, Staphylococcus and Streptococcus were isolated from the uterine washing fluid of assisted calving cows. Our results showed that the uterine microbiota of assisted calving and non-assisted calving cows were significantly different. Assisted calving resulted in increased diversity of uterine microflora in cows.

Key words: cow, assisted calving, uterine lavage fluid, microbiome

CLC Number:

S857.2

NING Xinnuan, LI Jie, FANG Hui, YU Siyuan, LIANG Jiaxi, ZHANG Lu, JIN Yaping, ZHOU Dong. Effect of Assisted Calving on Postpartum Uterine Flora of Dairy Cow[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4501-4506.

References 25

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Effect of Assisted Calving on Postpartum Uterine Flora of Dairy Cow

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