番茄红素对秦川牛精液冷冻保存及鲜精品质影响

doi:10.11843/j.issn.0366-6964.2022.12.036

Abstract

Abstract: The purpose of this experiment was to investigate the effect of lycopene on the semen cryopreservation and the fresh semen quality, and to provide a basis for lycopene as a new semen diluent additive and dietary additive for breeding males.Fresh semen from 3 healthy Qinchuan bulls aged 3 to 5 years old was collected and cryopreserved with diluents containing lycopene of 0, 1, 2, 3 and 4 μmol·L^-1, respectively. The motility, acrosome integrity, membrane integrity, mitochondrial activity and antioxidant capacity of sperms were measured by computer-aided analyzer, hypo-osmotic swelling test, peanut lectin fluorescent labeling assay, rhodamine fluorescence assay, and so on. Qinchuan bulls were fed with 15 mg·kg^-1 BW lycopene, fresh semen quality and serum antioxidant indexes were detected before and after feeding.The results showed that the supplementation of the semen extender with lycopene produced a higher sperm motility, acrosome integrity, membrane integrity, mitochondrial activity, CAT and GSH-Px enzyme activities, resulted in a lower content of MDA in sperm than that of the control, the optimal concentration of lycopene was 2 μmol·L^-1 (P<0.05). After 15 mg·kg^-1 BW lycopene supplementation in diet, compared with before feeding, the sperm motility of Qinchuan bulls had a significant elevation (P<0.05), the sperm deformity rate significantly decreased (P<0.05), and the activities of antioxidant enzymes such as SOD, CAT and GSH-Px significantly elevated (P<0.05), with the content of MDA significantly decreased in serum (P<0.05). In conclusion, a certain concentration of lycopene had a good protective effect on sperm damage caused by semen cryopreservation and the reproductive performance reduction caused by stress in Qinchuan bull, which might be illustrated by the potential function of lycopene on reducing the oxidative damage of sperm during the freezing-thawing process and improving the antioxidant capacity of the animal body.

Key words: lycopene, bull semen cryopreservation, semen quality, serum antioxidant index, antioxidation

CLC Number:

S823.3

WANG Meng, YANG Chaoqun, WU Silin, TAN Jianbing, DU Xinze, LI Zhenxing, ZAN Linsen, YANG Wucai. Impact of Lycopene on Semen Cryopreservation and Fresh Semen Quality of Qinchuan Bull[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4507-4517.

References 41

[1]	CONTRERAS M J,TREULEN F,ARIAS M E,et al.Cryopreservation of stallion semen:effect of adding antioxidants to the freezing medium on sperm physiology[J].Reprod Domest Anim,2020,55(2):229-239.
[2]	JANG H,KWON H J,SUN W S,et al.Effects of leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation[J].Vet Med Sci,2020,6(3):447-453.
[3]	汪俊跃,戴建军,张树山,等.高压均质鸡蛋卵黄对猪冷冻精子凋亡的影响[J].畜牧兽医学报,2021,52(8):2190-2199.WANG J Y,DAI J J,ZHANG S S,et al.Effects of high pressure homogeneous egg yolk on apoptosis of boar sperm cryopreservation[J].Acta Veterinaria et Zootechnica Sinica,2021,52(8):2190-2199.(in Chinese)
[4]	于广忠.影响种公牛精液品质因素分析[J].养殖与饲料,2022,21(1):19-21.YU G Z.Analysis of factors affecting the semen quality of breeding bulls[J].Animals Breeding and Feed,2022,21(1):19-21.(in Chinese)
[5]	SUN Y,GE X,LI X,et al.High-fat diet promotes renal injury by inducing oxidative stress and mitochondrial dysfunction[J].Cell Death Dis,2020,11(10):914.
[6]	吕松洁,付丽,范文华,等.线粒体靶向抗氧化剂Mitoquinone对湖羊冻精损伤的保护作用[J].畜牧兽医学报, 2019, 50(12): 2554-2559.LÜ S J,FU L,FAN W H,et al.Protective effect of mitochondria-targeted antioxidant Mitoquinone on the damage of hu sheep frozen sperm[J].Acta Veterinaria et Zootechnica Sinica,2019,50(12):2554-2559.(in Chinese)
[7]	储蓄,张军霞,王晶.动物氧化应激及其营养调控措施研究进展[J].畜牧兽医学报,2021,52(12):3346-3356.CHU X,ZHANG J X,WANG J.Research progress of animal oxidative stress and its nutritional regulation[J].Acta Veterinaria et Zootechnica Sinica,2021,52(12):3346-3356.(in Chinese)
[8]	樊懿萱,邓凯平,澹台文静,等.多不饱和脂肪酸日粮中添加酵母硒对湖羊脂肪酸组成和抗氧化的影响[J].畜牧兽医学报, 2018, 49(8):1661-1673.FAN Y X,DENG K P,TANTAI W J,et al.Effect of selenium yeast in containing PUFAs dietary on fatty acid composition and antioxidant capacity of Hu lambs[J].Acta Veterinaria et Zootechnica Sinica,2018,49(8):1661-1673.(in Chinese)
[9]	LI N,WU X T,ZHUANG W,et al.Tomato and lycopene and multiple health outcomes:umbrella review[J].Food Chem,2021, 343:128396.
[10]	左兆云.番茄红素的生理功能及其在动物生产中的应用进展[J].中国畜牧杂志,2021,57(2):40-45.ZUO Z Y.Advances in physiological functions of lycopene and its application in animal production[J].Chinese Journal of Animal Science, 2021,57(2):40-45.(in Chinese)
[11]	KHAN U M,SEVINDIK M,ZARRABI A,et al.Lycopene:food sources,biological activities,and human health benefits[J].Oxid Med Cell Longev,2021,2021:2713511.
[12]	杨少青,邵春艳,周彬,等.番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究[J].畜牧兽医学报,2022, 53(2): 637-645.YANG S Q,SHAO C Y,ZHOU B,et al.Protective effect and mechanism of lycopene on spleen Injury induced by nonylphenol in mice[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):637-645.(in Chinese)
[13]	MIRAHMADI M,AZIMI-HASHEMI S,SABURI E,et al.Potential inhibitory effect of lycopene on prostate cancer[J].Biomed Pharmacother,2020,129:110459.
[14]	NOURI M,AMANI R,NASR-ESFAHANI M,et al.The effects of lycopene supplement on the spermatogram and seminal oxidative stress in infertile men:a randomized,double-blind,placebo-controlled clinical trial[J].Phytother Res,2019,33(12): 3203-3211.
[15]	MENDIOLA J,TORRES-CANTERO A M,VIOQUE J,et al.A low intake of antioxidant nutrients is associated with poor semen quality in patients attending fertility clinics[J].Fertil Steril,2010,93(4):1128-1133.
[16]	ZHAO Y,LIN J,TALUKDER M,et al.Aryl hydrocarbon receptor as a target for lycopene preventing DEHP-induced spermatogenic disorders[J].J Agric Food Chem,2020,68(15):4355-4366.
[17]	MCPHERSON N O,SHEHADEH H,FULLSTON T,et al.Dietary micronutrient supplementation for 12 days in obese male mice restores sperm oxidative stress[J].Nutrients,2019,11(9):2196.
[18]	曲扬华,罗海玲,刘策,等.饲粮中添加番茄红素对绵羊生长发育、屠宰性能和血清抗氧化指标的影响[J].动物营养学报, 2017,29(4):1257-1264.QU Y H,LUO H L,LIU C,et al.Effects of lycopene supplementation on growth development,slaughter performance and serum antioxidant indices of sheep[J].Chinese Journal of Animal Nutrition,2017,29(4):1257-1264.(in Chinese)
[19]	王捷.番茄红素、芝麻酚和黄芩素对猪精子冷冻保存效果的影响[D].杨凌:西北农林科技大学,2017.WANG J.Effects of lycopene,sesamol and baicalein on cryopreservation of boar spematozoa[D].Yangling:Northwest A&F University.(in Chinese)
[20]	王艳华.四种抗氧化剂对山羊精液冷冻保存效果的影响[D].杨凌:西北农林科技大学,2016.WANG Y H.The Effects of four antioxidants on goat semen cryopreservation[D].Yangling:Northwest A&F University.(in Chinese)
[21]	梁作文,郭凯敏,戴小凡,等.番茄红素对人精子冷冻损伤的保护作用及机制研究[J].中华男科学杂志,2015,21(6):521-526.LIANG Z W,GUO K M,DAI X F,et al.Protective effect of lycopene on human spermatozoa during cryopreservation and its mechanism[J].National Journal of Andrology,2015,21(6):521-526.(in Chinese)
[22]	ZHANG P P,WANG J J,LI C Y,et al.Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress[J].Reprod Domest Anim,2020,55(9):1103-1114.
[23]	崔艳颖,史秀杰,张美佳,等.牛冷冻精液保存时间对精液品质及其受精能力的影响[J].中国畜牧杂志,2020,56(9):108-112.CUI Y Y,SHI X J,ZHANG M J,et al.Effects of storage time of bovine frozen semen on semen quality and fertilization ability[J].Chinese Journal of Animal Science,2020,56(9):108-112.(in Chinese)
[24]	WANG H T,LU P,LI Z Q,et al.Oligomeric proanthocyanidins and bamboo leaf flavonoids improve the quality of bull semen cryopreservation[J].Molecules,2022,27(3):1144.
[25]	吕春荣,权国波.白藜芦醇对绵羊冷冻精液质量的影响[J].中国畜牧兽医,2020,47(11):3611-3617.LÜ C R,QUAN G B.The effects of resveratrol on the quality of frozen sheep semen[J].China Animal Husbandry & Veterinary Medicine,2020,47(11):3611-3617.(in Chinese)
[26]	王艳华,朱海鲸,韩聪,等.番茄红素对山羊精液冷冻保存效果的影响[J].西北农业学报,2017,26(3):336-341.WANG Y H,ZHU H J,HAN C,et al.Effects of lycopene on cryopreservation of goat semen[J].Acta Agriculturae Boreali-Occidentalis Sinica,2017,26(3):336-341.(in Chinese)
[27]	姚怡心,刘萱,李志刚,等.天然抗氧化剂对亚麻籽甘油二酯油的抗氧化性能研究[J].中国油脂, 2022:1-11,doi:10.19902/ j.cnki.zgyz.1003-7969.210799.YAO Y X,LIU X,LI Z G,et al.Study on antioxidant properties of natural antioxidants on flaxseed-based diacylglycerol Oil[J]. China Oils and Fats,2022:1-11,doi:10.19902/j.cnki.zgyz.1003-7969.210799.(in Chinese)
[28]	ZHAO Z L,LIU Z,MAO X Z.Biotechnological advances in lycopene β-cyclases[J].J Agric Food Chem,2020,68(43): 11895-11907.
[29]	戴小凡.番茄红素对精子冷冻损伤的保护作用及机制的初步研究[D].长春:吉林大学,2014.DAI X F.A preliminary study about protective effects and mechanism by adding lycopene on human spermatozoa during cryopreservation[D].Changchun:Jilin University,2014.(in Chinese)
[30]	KANG H G,LEE S,JEONG P S,et al.Lycopene improves in vitro development of porcine embryos by reducing oxidative stress and apoptosis[J].Antioxidants (Basel),2021,10(2):230.
[31]	TVRDÁ E,KOVÁČIK A,TUŠIMOVÁ E,et al.Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa[J].J Anim Sci Biotechnol,2016,7(1):50.
[32]	TRIPATHY A,ARA F,GHOSH P,et al.Effect of lycopene on testicular androgenic and anti-oxidative enzymes in cyproterone acetate-induced male infertile Wistar strain albino rats:an in vitro study[J].Andrologia,2020,52(3):e13494.
[33]	张心壮,曹迪,格日乐其木格,等.畜禽生产中氧化应激分子调控机理及营养策略研究进展[J].畜牧与饲料科学, 2021, 42(4): 29-36.ZHANG X Z,CAO D,GERELCHIMEG,et al.Research advances on molecular regulatory mechanisms of oxidative stress and nutritional strategies in livestock and poultry production[J].Animal Husbandry and Feed Science,2021,42(4):29-36.(in Chinese)
[34]	车大璐,程素彩,张伟涛,等.热应激条件下藿朴蒲苓散对育肥羔羊生长性能、消化性能和血清生化指标的影响[J].畜牧兽医学报,2022,53(6):1829-1840.CHE D L,CHENG S C,ZHANG W T,et al.Effects of huopopuling powder on growth performance,digestibility and serum biochemical indexes in fattening lambs under heat stress[J].Acta Veterinaria et Zootechnica Sinica,2022,53(6):1829-1840.(in Chinese)
[35]	黄权,苏琳.动物体内氧化应激与抗氧化剂应用研究进展[J].中国兽药杂志,2013,47(5):66-69.HUANG Q,SU L.Progress of applied research on oxidative stress and antioxidants in animals[J].Chinese Journal of Veterinary Drug,2013,47(5):66-69.(in Chinese)
[36]	张金宝,唐姝,殷斌,等.维生素C抗畜禽热应激作用机理研究进展[J].畜牧与兽医,2019,51(1):136-140.ZHANG J B,TANG S,YIN B,et al.Progressin research on the mechanism of vitamin C resistance to heat stress in livestock and poultry[J].Animal Husbandry & Veterinary Medicine,2019,51(1):136-140.(in Chinese)
[37]	张玉.不同抗氧化剂的抗氧化特性及蛋鸡日粮应用效果比较研究[D].杨凌:西北农林科技大学,2016.ZHANG Y.Comparative study of antioxidant properties and the application effect on old laying hens about different kinds of antioxidant[D].Yangling:Northwest A&F University.(in Chinese)
[38]	MANGIAGALLI M G,MARTINO P A,SMAJLOVIC T,et al.Effect of lycopene on semen quality,fertility and native immunity of broiler breeder[J].Br Poult Sci,2010,51(1):152-157.
[39]	ATEŞŞAHIN A,KARAHAN İ,TÜRK G,et al.Protective role of lycopene on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats[J].Reprod Toxicol,2006,21(1):42-47.
[40]	靳天宇,牛宏泽,胡宇,等.日粮中添加亚硒酸钠对绵羊精液品质、线粒体活性和葡萄糖转运蛋白的影响[J].饲料工业, 2021, 42(9):41-46.JIN T Y,NIU H Z,HU Y,et al.Effects of dietary sodium selenite on semen quality,mitochondrial activity and glucose transporters in rams[J].Feed Industry,2021,42(9):41-46.(in Chinese)
[41]	SARKER M T,WAN X L,YANG H M,et al.Dietary lycopene supplementation could alleviate aflatoxin B1 induced intestinal damage through improving immune function and anti-oxidant capacity in broilers[J].Animals (Basel),2021,11(11):3165.

Impact of Lycopene on Semen Cryopreservation and Fresh Semen Quality of Qinchuan Bull

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 41

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Yating, XU Zejun, CHEN Hongyu, WANG Xianwei, CHEN Qixin, LIU Shenhe. Research Progress of Exogenous Vitamin E and Selenium Regulating Semen Quality in Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1389-1400.
[2]	ZHAO Zhixian, CHANG Xuerui, GUO Yong, LONG Cheng, SHENG Xihui, WANG Xiangguo, XING Kai, XIAO Longfei, LIN Zili, NI Hemin, QI Xiaolong. Research Progress on Nutritional Regulation of Semen Quality in Breeder Roosters [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2435-2443.
[3]	LIU Weiwei, ZHANG Yuxin, LI Xiumei, WANG Xiumin, ZHOU Weiwei, DAI Xiaofeng. Mechanism of Mori Folium in Improving Antioxidative Function of Chicken Based on Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1958-1970.
[4]	YANG Shaoqing, SHAO Chunyan, ZHOU Bin, SONG Quanjiang, WANG Xiaodu, SONG Houhui, JIANG Sheng. Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 637-645.
[5]	WANG Qi, WANG Changjian, WEI Zongyou, LU Hanxi, YAO Xiaolei, YANG Hua, WANG Feng, ZHANG Yanli. Study on the Role of AMPK Activator in Cryopreservation of Sheep Semen [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3033-3045.
[6]	LI Xiaobin, ZANG Changjiang, MA Jun, ZHAO Guodong, CHEN Hui, MENG Jun. Effect of Different Training Sites on Sports Performance, Antioxidant Index of Plasma and Glucose Metabolism of Yili Horse [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 517-523.
[7]	ZHAO Jun-xing, LIU Xiang-dong, JIN Ya-qian, LIU Wen-zhong, REN You-she, ZHANG Chun-xiang, ZHANG Jian-xin. Effect of Dietary Wine Grape Pomace Supplementation on the Antioxidant Ability and Meat Quality of Lambs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1481-1490.
[8]	GENG Hong-hong，ZHANG Jing-yang，LI Lian，WANG Gen-lin. Semen Quality Analysis of Betelnut-jiang Buffalo in Different Seasons by RNA-seq [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1373-1380.
[9]	ZHANG Chun-xiang，ZHAO Hui，GUO Li-na，ZHENG Ya-lin，ZHANG Cai-xia，LIU Wen-zhong，QIAO Li-ying，REN You-she. Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1354-1362.
[10]	HUANG Li-na，JIANG Hui-hui，WANG Jie，GAO Ting，ZHANG Ye-fei，WU Bao-cheng，WU Yi-jian，HUANG Yi-fan. Effects of Hericium erinaceus Polysaccharide on Intestinal Mucosal Immune and Antioxidant Capacity of Muscovy Ducks Infected with Reovirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1671-1677.
[11]	HAO Hai-sheng，REN Jing-jing，ZHAO Xue-ming，WANG Ling-ling，DU Wei-hua，WANG Chang-fa，ZHU Hua-bin. Effects of the Degree of Apoptosis in Sexed Sperms on Bovine Fertilization in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 893-900.
[12]	LI Qiu-feng，LI Jian-guo，WU Yun-hai，MA Ya-bin，LIU Xing-hua，GAO Yan-xia，CAO Yu-feng，LIU Ting-yu. The Effects of Vitamin E，Beta-carotene and Zinc on Semen Quality，Antioxidant Indicators in Hot Weather [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 587-595.
[13]	XIE Xiangxue;MENG Qingxiang;REN Liping;ZHANG Xinzhuang;LIU Ping;. Study on the Antioxidant Ability in Longissimus doris of Different Breeds of Beef Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(4): 534-552.
[14]	SUN Tao;DAI La;TANG Feijiang;XIE Peng;ZOU Xiaoting. Effects of Dietary Cadmium on Production Performance，Antioxidative Capacity and the Retention of Laying Hens [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(2): 232-241.
[15]	ZOU Xiaoting;HU Jiacheng; CAO Derui;DONG Jinge. Effect of γ-Aminobutyric Acid on Growth Performance, Antioxidation and Hormones of HPA, HPT Axis in Growthfinishing Swine in Hot Summer [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(8): 1196-1201.