基于SourceTracker分析牧场环境对乳房炎乳菌群的影响

doi:10.11843/j.issn.0366-6964.2023.09.026

Abstract

Abstract: Bovine mastitis is the most economically important disease of dairy cattle, which is most commonly caused by pathogens. The pathogens that influenced dairy cows might be from the farm environment. Therefore, the flow of microbes through environments to cow mastitis should be investigated. In this study, mastitis milk, pan barn samples (air, drinking water, feces, feed, bedding material, new bedding material, spray water), and parlour samples (pre teat medicine, post teat medicine, pre medicine cup, post medicine cup, teat dip cup, teat skin) have been collected in a dairy farm in Tianjin, China. Total DNA of culture-based and nonculture-based samples were extracted and then performed 16S rDNA amplicon sequencing and SourceTracker. OTU profiles were assigned to 50 families among all the 74 samples. Beta diversity and ANOSIM analysis showed that the similarity of bacteria between the mastitis milk and environment samples was low. In general, the microbial community structure was similar between mastitis milk and teat skin and, to a lesser extent, bedding samples. Moreover, SourceTracker identified the teat skin as the most important source of pollution, followed by air, milk cups and feces, which highlighted the possible effects of farm management practices on bovine mastitis. Therefore, control measures should be introduced to reduce or eliminate the risk of transmission in the dairy farms.

Key words: dairy cow mastitis, pan barn, parlour, 16S rDNA sequencing, SourceTracker

CLC Number:

Q938.11

MENG Lu, HU Haiyan, DONG Lei, ZHENG Nan, WANG Jiaqi. Influence of Dairy Farm Environment on Mastitis Milk Microbiota via SourceTracker[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3872-3883.

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Influence of Dairy Farm Environment on Mastitis Milk Microbiota via SourceTracker

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