奶牛早期妊娠诊断蛋白的研究

doi:10.11843/j.issn.0366-6964.2024.02.004

Abstract

Abstract: Pregnancy failure caused by early embryonic death in dairy cows can result in significant economic losses to the dairy farm. Early and accurate pregnancy diagnosis in non-pregnant cows will improve the efficiency of pregnancy and shorten the number of days to nulliparity. This paper reviews recent domestic and international studies on pregnancy-associated proteins, body fluid proteins such as blood, urine, and milk of cows in early pregnancy stages, and proteomics to identify potential proteins in early non-pregnancy for accurate pregnancy diagnosis in early pregnancy and to improve dairy production efficiency.

Key words: dairy cows, proteomics, early pregnancy diagnosis, biomarkers, pregnancy-related substances

CLC Number:

S823.91

ZHANG Xinrui, FU Yu, YANG Zhuo, SHEN Wenjuan, TAO Jinzhong. Study of Early Pregnancy Diagnostic Proteins in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 451-460.

References

[1] 冯肖艺,郝海生,杜卫华,等.能量负平衡导致奶牛繁殖力下降的机制进展[J].畜牧兽医学报,2023,54(10):4050-4060.
FENG X Y,HAO H S,DU W H,et al.Progress in mechanism of reduced fertility in dairy cows due to negative energy balance[J].Acta Veterinaria et Zootechnica Sinica,2023,54(10):4050-4060.(in Chinese)
[2] SMITH M F,GEISERT R D,PARRISH J J.Reproduction in domestic ruminants during the past 50 yr:discovery to application[J].J Anim Sci,2018,96(7):2952-2970.
[3] FORDE N,LONERGAN P.Interferon-tau and fertility in ruminants[J].Reproduction,2017,154(5):F33-F43.
[4] SPECKHART S L,OLIVER M A,EALY A D.Developmental hurdles that can compromise pregnancy during the first month of gestation in cattle[J].Animals (Basel),2023,13(11):1760.
[5] 罗芳,张萌,李亚超,等.影响奶牛早期胚胎丢失的因素[J].畜牧兽医学报,2020,51(5):907-913.
LUO F,ZHANG M,LI Y C,et al.Factors associated with early embryo loss in dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2020,51(5):907-913.(in Chinese)
[6] 赵璐.山羊早期胚胎基因组激活过程中高表达lncRNA的筛选和功能研究[D].杨凌:西北农林科技大学,2020.
ZHAO L.Screening and functional study of highly expressed lncRNA during embryonic genome activation in goat[D].Yangling:Northwest A&F University,2020.(in Chinese)
[7] BERG D K,LEDGARD A,DONNISON M,et al.The first week following insemination is the period of major pregnancy failure in pasture-grazed dairy cows[J].J Dairy Sci,2022,105(11):9253-9270.
[8] ADHIKARI B,LEE C N,KHADKA V S,et al.RNA-Sequencing based analysis of bovine endometrium during the maternal recognition of pregnancy[J].BMC Genomics,2022,23(1):494.
[9] GARCIA-ISPIERTO I,LÓPEZ-HELGUERA I,SERRANO-PÉREZ B,et al.Progesterone supplementation during the time of pregnancy recognition after artificial insemination improves conception rates in high-producing dairy cows[J].Theriogenology,2016,85(7):1343-1347.
[10] 魏士昊,戴天姝,王锋,等.奶牛早孕诊断技术研究进展[J].畜牧兽医学报,2022,53(7):2047-2056.
WEI S H,DAI T S,WANG F,et al.Research progress of early pregnancy diagnosis in dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2022,53(7):2047-2056.(in Chinese)
[11] EALY A D,SEEKFORD Z K.Symposium review:predicting pregnancy loss in dairy cattle[J].J Dairy Sci,2019,102(12):11798-11804.
[12] JAŚKOWSKI J M,KACZMAROWSKI M,KULUS J,et al.Rectal palpation for pregnancy checks in cows-the past or an alternative to modern diagnostic methods[J].Med Weter,2019,75(1):6156-2019.
[13] SAMIR H,KANDIEL M M M.Accuracy of subjective evaluation of luteal blood flow by color Doppler ultrasonography for early diagnosis of pregnancy in Egyptian buffalo[J].Anim Reprod Sci,2019,208:106129.
[14] 李福慧.规模化奶牛场B超早期妊娠诊断规程的研究[D].呼和浩特:内蒙古农业大学,2021.
LI F H.Research on Early pregnancy diagnosis procedure by ultrasonography in scaled dairy farm[D].Hohhot:Inner Mongolia Agricultural University,2021.(in Chinese)
[15] FRICKE P M,RICCI A,GIORDANO J O,et al.Methods for and implementation of pregnancy diagnosis in dairy cows[J].Vet Clin North Am Food Anim Pract,2016,32(1):165-180.
[16] 苏妮,孙振师,张化恒.奶牛三种早孕诊断方法的比较[J].山东畜牧兽医,2012,33(4):17-18.
SU N,SUN Z S,ZHANG H H.Comparison of three methods for diagnosing early pregnancy in dairy cows[J].Shandong Journal of Animal Science and Veterinary Medicine,2012,33(4):17-18.(in Chinese)
[17] 李助南,唐登华,郭良辉.血小板计数法对奶牛超早期妊娠的诊断研究[J].黑龙江畜牧兽医,2005(9):15-16.
LI Z N,TANG D H,GUO L H.A diagnostic study of platelet count in ultra-early pregnancy in dairy cows[J].Heilongjiang Animal Science and Veterinary Medicine,2005(9):15-16.(in Chinese)
[18] 罗芳,陶金忠.基于UHPLC-QTOF-MS代谢组学方法筛选奶牛不同妊娠状态时的候选生物标志物[J].畜牧兽医学报,2021,52(11):3118-3125.
LUO F,TAO J Z.Screen of candidate biomarkers for different pregnancy status of cows based on UHPLC-QTOF-MS metabolomics[J].Acta Veterinaria et Zootechnica Sinica,2021,52(11):3118-3125.(in Chinese)
[19] FORDE N,SIMINTIRAS C A,STURMEY R,et al.Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle[J].PLoS One,2014,9(6):e100010.
[20] 原开敏,时玉新,董智豪,等.母牛早期妊娠诊断技术研究进展[J].畜牧兽医学报,2023,54(6):2223-2230.
YUAN K M,SHI Y X,DONG Z H,et al.Research progress on early pregnancy diagnosis technologies in cows[J].Acta Veterinaria et Zootechnica Sinica,2023,54(6):2223-2230.(in Chinese)
[21] FAN X G,ZHENG Z Q,FAN X G,et al.A study of early pregnancy factor activity in preimplantation[J].Am J Reprod Immunol,1997,37(5):359-364.
[22] 白云.奶牛四种妊娠诊断方法与结果的比较[D].石河子:石河子大学,2013.
BAI Y.The diagnostic results comparison of four pregnancy diagnostic methods in cows[D].Shihezi:Shihezi University,2013.(in Chinese)
[23] ROMERO J J,ANTONIAZZI A Q,NETT T M,et al.Temporal release,paracrine and endocrine actions of ovine conceptus-derived interferon-tau during early pregnancy[J].Biol Reprod,2015,93(6):146.
[24] GREEN J A,PARKS T E,AVALLE M P,et al.The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers[J].Theriogenology, 2005,63(5):1481-1503.
[25] 赵生贤,樊江峰,余四九,等.早孕因子(EPF)在雌性牦牛主要生殖器官中的表达[J].农业生物技术学报,2020,28(5):876-883.
ZHAO S X,FAN J F,YU S J,et al.Expression of early pregnancy factors (EPF) in the main reproductive organs of female yak (Bos grunnien)[J].Journal of Agricultural Biotechnology,2020,28(5):876-883.(in Chinese)
[26] NAHHAS F,BARNEA E.Human embryonic origin early pregnancy factor before and after implantation[J]. Am J Reprod Immunol,1990,22(3-4):105-108.
[27] GROSSO M C,BELLINGERI R V,MOTTA C E,et al.Immunohistochemical distribution of early pregnancy factor in ovary,oviduct and placenta of pregnant gilts[J].Biotech Histochem,2015,90(1):14-24.
[28] OTT T L.Immunological detection of pregnancy:evidence for systemic immune modulation during early pregnancy in ruminants[J].Theriogenology,2020,150:498-503.
[29] 李雯乔,李发弟,王新基,等.家畜早期妊娠诊断的研究进展[J].畜牧兽医学报,2023,54(5):1782-1791.
LI W Q,LI F D,WANG X J,et al.Advance of early pregnancy diagnosis in livestock[J].Acta Veterinaria et Zootechnica Sinica,2023,54(5):1782-1791.(in Chinese)
[30] LALEH V G,LALEH R G,PIRANY N,et al.Measurement of EPF for detection of cow pregnancy using rosette inhibition test[J].Theriogenology,2008,70(1):105-107.
[31] 张宏刚,陈树林,王晓珊,等.孕牛血清早孕因子的生物活性检测及妊娠率分析[J].动物医学进展,2010,31(8):30-33.
ZHANG H G,CHEN S L,WANG X S,et al.Detection of biological activity of EPF from sera of pregnant cows and analysis of the pregnant rate[J].Progress in Veterinary Medicine,2010,31(8):30-33.(in Chinese)
[32] 刘水喜.奶牛早期妊娠诊断几种方法的比较[J].畜禽业,2016,27(12):35-36.
LIU S X.Comparison of several methods for early pregnancy diagnosis in dairy cows[J].Livestock and Poultry Industry,2016,27(12):35-36.(in Chinese)
[33] HAN X,WANG S Y,REN Y,et al.Selection of early pregnancy specific proteins and development a rapid immunochromatographic test strip in cows[J].Theriogenology,2022,187:127-134.
[34] LIU J F,WU Z M,GUO S,et al.IFN-τ attenuates LPS-induced endometritis by restraining HMGB1/NF-κB activation in bEECs[J].Inflammation,2021,44(4):1478-1489.
[35] HIRAYAMA H,MORIYASU S,KAGEYAMA S,et al.Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer[J].Theriogenology,2014,81(8):1108-1115.
[36] TOJI N,SHIGENO S,KIZAKI K,et al.Evaluation of interferon-stimulated genes in peripheral blood granulocytes as sensitive responders to bovine early conceptus signals[J].Vet J,2017,229:37-44.
[37] SUN M,HAO F,LI W L,et al.Temporal dynamics of the ruminant type I IFN-induced antiviral state against homologous parainfluenza virus 3 challenge in vitro[J].Viruses,2022,14(5):1025.
[38] HARA K,SHIRASUNA K,USUI F,et al.Interferon-tau attenuates uptake of nanoparticles and secretion of interleukin-1β in macrophages[J].PLoS One,2014,9(12):e113974.
[39] 朱喆.干扰素-τ调节妊娠早期奶牛子宫内膜BoLA-Ⅰ机制研究[D].武汉:华中农业大学,2017.
ZHU Z.The mechanism of interferon-τ regulate endometrial BoLA-Ⅰ during early pregnancy in dairy cows[D].Wuhan:Huazhong Agricultural University,2017.(in Chinese)
[40] 程蕾,王定发,刘晓华,等.ISG15和OAS1在奶牛早期妊娠阶段外周血中的表达规律[J].畜牧兽医学报,2015,46(1):77-84.
CHENG L,WANG D F,LIU X H,et al.Study on ISG15 and OAS1 transcriptions in peripheral blood of dairy cows during early pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2015,46(1):77-84.(in Chinese)
[41] CASANO A B,BARILE V L,MENCHETTI L,et al.Interferon tau (IFNt) and interferon-stimulated genes (ISGs) expression in peripheral blood leukocytes and correlation with circulating pregnancy-associated glycoproteins (PAGs) during peri-implantation and early pregnancy in buffalo cows[J].Animals (Basel), 2022, 12(22):3068.
[42] PUGLIESI G,MIAGAWA B T,PAIVA Y N,et al.Conceptus-induced changes in the gene expression of blood immune cells and the ultrasound-accessed luteal function in beef cattle:how early can we detect pregnancy?[J].Biol Reprod,2014,91(4):95.
[43] DE MELO G D,MELLO B P,FERREIRA C A,et al.Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle[J]. Theriogenology, 2020,152:94-105.
[44] YOSHINO H,TOJI N,SASAKI K,et al.A predictive threshold value for the diagnosis of early pregnancy in cows using interferon-stimulated genes in granulocytes[J].Theriogenology,2018,107:188-193.
[45] POHLER K G,REESE S T,FRANCO G A,et al.New approaches to diagnose and target reproductive failure in cattle[J].Anim Reprod,2020,17(3):e20200057.
[46] 骆金红,陈祥,尚以顺,等.转录组测序筛选山羊妊娠早期胚胎附植相关基因[J].畜牧兽医学报,2022,53(5):1465-1474.
LUO J H,CHEN X,SHANG Y S,et al.Transcriptome sequencing screening the genes related to goat embryo attachment in early pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2022,53(5):1465-1474.(in Chinese)
[47] GIORDANO J O,GUENTHER J N,LOPES G,et al.Changes in serum pregnancy-associated glycoprotein,pregnancy-specific protein B,and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows[J].J Dairy Sci,2012,95(2):683-697.
[48] REESE S T,PEREIRA M H C,EDWARDS J L,et al.Pregnancy diagnosis in cattle using pregnancy associated glycoprotein concentration in circulation at day 24 of gestation[J].Theriogenology,2018,106:178-185.
[49] 钟旭,薛立群,田见晖.妊娠相关糖蛋白(PAG)在奶牛早期妊娠诊断上的应用现状[J].中国畜牧兽医,2011,38(11):140-143.
ZHONG X,XUE L Q,TIAN J H.Early pregnancy diagnosis by pregnancy associated glycoprotein in dairy cattle[J].China Animal Husbandry & Veterinary Medicine,2011,38(11):140-143.(in Chinese)
[50] FRIEDRICH M,HOLTZ W.Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection[J].Reprod Domest Anim,2010,45(1):142-146.
[51] DUROCHER J,MOORE R K,CASTONGUAY M H,et al.Bayesian estimation of sensitivity and specificity of a milk pregnancy-associated glycoprotein ELISA test for pregnancy diagnosis between 23 and 27 days after insemination in Holstein dairy cows[J].J Dairy Sci,2022,105(8):6985-6996.
[52] POHLER K G,PEREIRA M H C,LOPES F R,et al.Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds[J].J Dairy Sci,2016,99(2):1584-1594.
[53] FRISÉE V,RIGAUX G,DUFOUR P,et al.American Bison (Bison bison) reproductive endocrinology:serum Pregnancy Associated Glycoproteins (PAG),Progesterone,Estrone and Estrone-Sulfate in non pregnant animals and during gestation[J].Domest Anim Endocrinol,2022,78:106684.
[54] VELEK K,MICHAUD S,BOUCHER K,等.爱德士牛怀孕检测[C]//中国奶业协会第26次繁殖学术年会暨国家肉牛牦牛/奶牛产业技术体系第3届全国牛病防治学术研讨会论文集.兰州,2011:140-143.
VELEK K,MICHAUD S,BOUCHER K,et al.IDEXX cattle pregnancy test[C]//Proceedings of the 26th Annual Conference of Breeding of China Dairy Association and the 3rd National Symposium on Cattle Disease Prevention and Control of National Beef Cattle,Yak/Dairy Cattle Industry Technology System.Lanzhou,2011:140-143.(in Chinese)
[55] BAHUGUNA C,SHARMA M.Proteomic analysis of serum protein during early pregnancy[J].J Proteins Proteomics,2022,13(1):1-15.
[56] PANDA B S K,MOHAPATRA S K,CHAUDHARY D,et al.Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows[J].J Reprod Immunol,2020, 140:103148.
[57] JOHNSTON D,MALO ESTEPA I,EBHARDT H A,et al.Differences in the bovine milk whey proteome between early pregnancy and the estrous cycle[J].Theriogenology,2018,114:301-307.
[58] RAWAT P,BATHLA S,BAITHALU R,et al.Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation[J].Clin Proteomics,2016,13:15.
[59] ARANCIAGA N,MORTON J D,MAES E,et al.Proteomic determinants of uterine receptivity for pregnancy in early and mid-postpartum dairy cows[J].Biol Reprod,2021,105(6):1458-1473.
[60] MORAES J G N,BEHURA S K,BISHOP J V,et al.Analysis of the uterine lumen in fertility-classified heifers:II.Proteins and metabolites[J].Biol Reprod,2020,102(3):571-587.
[61] KIZAKI K,SHICHIJO-KIZAKI A,FURUSAWA T,et al.Differential neutrophil gene expression in early bovine pregnancy[J].Reprod Biol Endocrinol,2013,11:6.
[62] 余婕,向敏,刘辰晖,等.干扰素-τ调控牛母体妊娠识别分子机制的研究进展[J].中国奶牛,2021(8):28-32.
YU J,XIANG M,LIU C H,et al.Research progress on molecular mechanism of maternal recognition of pregnancy regulated by interferon-tau in cows[J].China Dairy Cattle,2021(8):28-32.(in Chinese)
[63] SZELÉNYI Z,SZENCI O,BODÓ S,et al.Noninfectious causes of pregnancy loss at the late embryonic/early fetal stage in dairy cattle[J].Animals (Basel),2023,13(21):3390.
[64] HAN R X,KIM H R,DIAO Y F,et al.Detection of early pregnancy-specific proteins in Holstein milk[J].J Proteomics,2012,75(11):3221-3229.
[65] KUMAR R,ALI S A,SINGH S K,et al.Peptide profiling in cow urine reveals molecular signature of physiology-driven pathways and in-silico predicted bioactive properties[J].Sci Rep,2021,11(1):12427.
[66] SPENCER T E,FORDE N,LONERGAN P.Insights into conceptus elongation and establishment of pregnancy in ruminants[J].Reprod Fertil Dev,2017,29(1):84-100.
[67] LAMY J,LABAS V,HARICHAUX G,et al.Regulation[LL]of the bovine oviductal fluid proteome[J]. Reproduction, 2016,152(6):629-644.
[68] MYERS T D,PALLADINO M J.Newly discovered roles of triosephosphate isomerase including functions within the nucleus[J].Mol Med,2023,29(1):18.
[69] BELTMAN M E,MULLEN M P,ELIA G,et al.Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos[J].Domest Anim Endocrinol, 2014, 46:49-57.
[70] ARTUS J,HUE I,ACLOQUE H.Preimplantation development in ungulates:a 'ménage à quatre' scenario[J]. Reproduction,2020,159(3):R151-R172.
[71] 张航,杨柏高,徐茜,等.热应激影响奶牛胚胎发育作用机制的研究进展[J].畜牧兽医学报,2023, 54(7):2692-2700.
ZHANG H,YANG B G,XU X,et al.Research progress on the mechanism of heat stress affecting the development of dairy cow embryos[J].Acta Veterinaria et Zootechnica Sinica,2023,54(7):2692-2700.(in Chinese)
[72] MUÑOZ M,CORRALES F J,CAAMANÑO J N,et al.Proteome of the early embryo-maternal dialogue in the cattle uterus[J].J Proteome Res,2012,11(2):751-766.

Study of Early Pregnancy Diagnostic Proteins in Dairy Cows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[2]	ZHAO Wanli, CAO Qiqi, YANG Yue, DENG Zhaoju, XU Chuang. The Interaction between Gastrointestinal Microbiota and Mucosal Immunity in Health of Perinatal Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2751-2760.
[3]	YUAN Kaimin, SHI Yuxin, DONG Zhihao, ZHAO Lingjun, XU Shiyuan, WU Kaihui, WANG Dong. Research Progress on Early Pregnancy Diagnosis Technologies in Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2223-2230.
[4]	HUANG Shangzhen, MA Longgang, LOU Wenqi, NING Jingyang, ZHANG Hailiang, HU Lirong, ZHA Qiong, LI Bin, XU Qing, BASANG Luobu, WANG Yachun. Analysis of Influencing Factors on Blood Indicators of Dairy Cows at High-altitude Area [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1964-1978.
[5]	FENG Xiaoyi, YANG Baigao, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Mechanism and Solution of Heat Stress Induced Embryo Quality Decline in Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 868-876.
[6]	PAN Chanyuan, ZHAO Zixuan, DUAN Mingjie, JIANG Linshu, TONG Jinjin. The Mechanism of Artemisia carvifolia Alleviating Dairy Cow Oxidative Stress Predicted by Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1071-1084.
[7]	HE Wenfeng, LI Chen, CHANG Hongtao, LI Longxi, CHEN Jing, YANG Guoqing, LIU Huimin. Screening and Identifying of Host Proteins that Inhibit Pseudorabies Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1177-1186.
[8]	FAN Lei, SHEN Yu, YOU Liuchao, TIAN Xinyu, LUO Hao, WANG Xin, ZHANG Tingting, SHEN Liuhong. Research Progress on Abnormal Glucose and Lipid Metabolism in Dairy Cows Induced by Lipopolysaccharide (LPS) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 484-493.
[9]	SHI Rui, SU Guosheng, CHEN Ziwei, LI Xiang, LUO Hanpeng, LIU Lin, GUO Gang, ZHANG Yi, WANG Yachun, ZHANG Shengli, ZHANG Qin. Comparisons of Genomic Predictions for Fertility Traits in Chinese Holstein Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2944-2954.
[10]	YAN Shuo, ZHAO Shanshan, ZHU Zhendong, PAN Qingjie, DONG Huansheng. Study on Nuclear-plasma Transporter KPNA4 of Sheep Sperm Cell [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2194-2201.
[11]	QI Mengfan, XIE Su, GAO Ruonan, SUN Yishan, SUN Xiaomei, HE Junfei, LU Huiwen, LU Shihao, CHEN Xin, LI Qingchun, HUANG Tao. Identification of Differentially Expressed Proteins in Blood of Sows at Early Pregnancy [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1109-1121.
[12]	DOU Mengying, ZHANG Cai, LI Yuanxiao, SHAO Qi, ZHU Jiali, LI Wang, CAO Zhijun. Effects of L-Arginine on Proliferation and Apoptosis of Primary Intestinal Epithelial Cells of Dairy Cows Treated with Heat Stress in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 493-504.
[13]	FU Ming, HE Junjun, ZHU Xingquan, CONG Wei. Proteomic Analysis of Changes in the Mouse Brain Tissue Infected with Toxoplasma gondii Oocysts during the Acute and Chronic Stage [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 556-566.
[14]	ZHANG Anrong, WU Zhengke, CHEN Zhimin, CHANG Wenhuan, CAI Huiyi, LIU Guohua, ZHENG Aijuan. Unraveling Molecular Mechanism of Acute Immunological Stress Affecting Meat Quality of Broiler Chickens by Proteomics Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2138-2150.
[15]	LUO Fang, TAO Jinzhong. Screen of Candidate Biomarkers for Different Pregnancy Status of Cows Based on UHPLC-QTOF-MS Metabolomics [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3118-3125.