共轭亚油酸对奶牛乳脂肪球粒径及分布的影响

doi:10.11843/j.issn.0366-6964.2021.05.029

Abstract

Abstract: The aim of this study was to evaluate the effect of conjugated linoleic acid (CLA) on the size and distribution of milk fat globule(MFG) of dairy cows. Twenty-four Holstein cows with similar body condition in mid-lactation(body weight (583±34.6) kg，milk yield (27.2±2.4) kg·d^-1) were randomly assigned to 4 groups, 6 replicate in each group, and one cow in each replicate. The individuals in control group (C group) were fed with basal diet, the individuals in low dose CLA group (L group) were fed with basal diet + 150 g·d^-1 CLA, individuals in medium dose CLA group (M group) were fed with basal diet + 300 g·d^-1 CLA, individuals in high dose CLA group (H group) were fed with basal diet + 400 g·d^-1 CLA. Through 5 d treatment, dry matter intake(DMI) and milk yield were recorded and milk components were analyzed. The average diameter and particle size distribution of MFG were measured by Mastersizer 3000 laser particle size analyzer. The results showed that CLA supplement did not significantly affect the DMI, milk yield, content of milk protein and milk lactose(P>0.05), but extremely significantly reduced milk fat content (P<0.01). The mean particle size D_[4,3]and D_[3,2] of MFG in CLA group cows were significantly lower than those in the control group cows(P<0.05). Furthermore, CLA supplementation decreased the distribution percentage of large globule particles, and increased the distribution percentage of small globule particles. In this experiment, it can be concluded that dietary CLA levels affect size and distribution of MFG, which provides a basis for clarifying the mechanism of milk fat depression caused by CLA.

Key words: dairy cow, lactation, conjugated linoleic acid, milk fat globule size

CLC Number:

HUANG Qixue, ZHANG Menglu, ZHANG Zhijin, XING Zhiyang, ZHU Heshui, WANG Yueying, FU Tong, GAO Tengyun, HAN Liqiang. Effect of Conjugated Linoleic Acid on the Size and Distribution of Milk Fat Globule in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1432-1438.

References 34

[1]	BERNARD L,LEROUX C,CHILLIARD Y.Expression and nutritional regulation of lipogenic genes in the ruminant lactating mammary gland[J].Adv Exp Med Biol,2008,606(1):67-108.
[2]	BAUMAN D E,GRIINARI J M.Regulation and nutritional manipulation of milk fat:low-fat milk syndrome[J].Livest Prod Sci,2001,70(1-2):15-29.
[3]	LOOR J J,HERBEIN J H.Exogenous conjugated linoleic acid isomers reduce bovine milk fat concentration and yield by inhibiting de novo fatty acid synthesis[J].J Nutr,1998,128(12):2411-2419.
[4]	HUANG Y,SCHOONMAKER J P,BRADFORD B J,et al.Response of milk fatty acid composition to dietary supplementation of soy oil,conjugated linoleic acid,or both[J].J Dairy Sci,2008,91(1):260-270.
[5]	LOOR J J,HERBEIN J H.Reduced fatty acid synthesis and desaturation due to Exogenus trans10,cis12-CLA in cows fed oleic or linoleic oil[J].J Dairy Sci,2003,86(4):1354-1369.
[6]	HARVATINE K J,BAUMAN D E.SREBP1 and thyroid hormone responsive spot 14(S14) are involved in the regulation of bovine mammary lipid synthesis during diet-induced milk fat depression and treatment with CLA[J].J Nutr,2006,136(10):2468-2474.
[7]	LEE H,PADHI E,HASEGAWA Y,et al.Compositional dynamics of the milk fat globule and its role in infant development[J].Front Pediatr,2018,6:313.
[8]	MICHALSKI M C,BRIARD V,MICHEL F,et al.Size distribution of fat globules in human colostrum,breast milk,and infant formula[J].J Dairy Sci,2005,88(6):1927-1940.
[9]	ARGOV-ARGAMAN N,MIDA K,COHEN B C,et al.Milk fat content and DGAT1 genotype determine lipid composition of the milk fat globule membrane[J].PLoS One,2013,8(7):e68707.
[10]	MESILATI-STAHY R,ARGOV-ARGAMAN N.The relationship between size and lipid composition of the bovine milk fat globule is modulated by lactation stage[J].Food Chem,2014,145:562-570.
[11]	LOPEZ C,BRIARD-BION V,MENARD O,et al.Phospholipid,sphingolipid,and fatty acid compositions of the milk fat globule membrane are modified by diet[J].J Agric Food Chem,2008,56(13):5226-5236.
[12]	MESILATI-STAHY R,MIDA K,ARGOV-ARGAMAN N.Size-dependent lipid content of bovine milk fat globule and membrane phospholipids[J].J Agric Food Chem,2011,59(13):7427-7435.
[13]	LIANG L,QI C,WANG X G,et al.Influence of homogenization and thermal processing on the gastrointestinal fate of bovine milk fat:in vitro digestion study[J].J Agric Food Chem,2017,65(50):11109-11117.
[14]	ARGOV-ARGAMAN N,MIDA K,COHEN B C,et al.Milk fat content and DGAT1 genotype determine lipid composition of the milk fat globule membrane[J].PLoS One,2013,8(7):e68707.
[15]	LU J,ARGOV-ARGAMAN N,ANGGREK J,et al.The protein and lipid composition of the membrane of milk fat globules depends on their size[J].J Dairy Sci,2016,99(6):4726-4738.
[16]	LOPEZ C,MÉNARD O.Human milk fat globules:polar lipid composition and in situ structural investigations revealing the heterogeneous distribution of proteins and the lateral segregation of sphingomyelin in the biological membrane[J].Colloids Surf B Biointerfaces,2011,83(1):29-41.
[17]	MICHALSKI M C,GASSI J Y,FAMELART M H,et al.The size of native milk fat globules affects physic-chemical and sensory properties of Camembert cheese[J].Dairy Sci Technol,2003,83(2):131-143.
[18]	AVRAMIS C A,WANG H,MCBRIDE B W,et al.Physical and processing properties of milk,butter,and Cheddar cheese from cows fed supplemental fish meal[J].J Dairy Sci,2003,86(8):2568-2576.
[19]	MICHALSKI M C,OLLIVON M,BRIARD V,et al.Native fat globules of different sizes selected from raw milk:thermal and structural behavior[J].Chem Phys Lipids,2004,132(2):247-261.
[20]	ARGOV N,LEMAY D G,GERMAN J B.Milk fat globule structure and function:nanoscience comes to milk production[J].Trends Food Sci Tech,2008,19(12):617-623.
[21]	PERFIELD II J W,BERNAL-SANTOS G,OVERTON T R,et al.Effects of dietary supplementation of rumen-protected conjugated linoleic acid in dairy cows during established lactation[J].J Dairy Sci,2002,85(10): 2609-2617.
[22]	VISWANADHA S,GIESY J G,HANSON T W,et al.Dose response of milk fat to intravenous administration of the trans-10,cis-12 isomer of conjugated linoleic acid[J].J Dairy Sci,2003,86(10):3229-3236.
[23]	MCMANAMAN J L,RUSSELL T D,SCHAACK J,et al.Molecular determinants of milk lipid secretion[J].J Mammary Gland Biol Neop,2007,12(4):259-268.
[24]	WALTHER T C,FARESE JR R V.Lipid droplets and cellular lipid metabolism[J].Annu Rev Biochem,2012, 81(1): 687-714.
[25]	ARGOV-ARGAMAN N.Symposium review:Milk fat globule size:Practical implications and metabolic regulation[J].J Dairy Sci,2019,102(3):2783-2795.
[26]	ALTENHOFER C,HOLZMÜLLER W,WOLFERTSTETTER F,et al.Temporal variation of milk fat globule diameter,fat and cholesterol content and milk epithelial cell gene expression in dairy cows[J].Int J Dairy Technol, 2015,68(4):519-526.
[27]	GERVAIS R,MCFADDEN J W,LENGI A J,et al.Effects of intravenous infusion of trans-10,cis-1218:2 on mammary lipid metabolism in lactating dairy cows[J].J Dairy Sci,2009,92(10):5167-5177.
[28]	HAN L Q,PANG K,LI H J,et al.Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands[J].Genet Mol Res,2012,11(4):4754-4764.
[29]	HARVATINE K J,BOISCLAIR Y R,BAUMAN D E.Time-dependent effect of trans-10,cis-12 conjugated linoleic acid on gene expression of lipogenic enzymes and regulators in mammary tissue of dairy cows[J].J Dairy Sci, 2018,101(8):7585-7592.
[30]	WALTHER T C,CHUNG J,FARESE JR R V.Lipid droplet biogenesis[J].Annu Rev Cell Dev Biol,2017,33: 491-510.
[31]	COHEN B C,RAZ C,SHAMAY A,et al.Lipid droplet fusion in mammary epithelial cells is regulated by phosphatidylethanolamine metabolism[J].J Mammary Gland Biol Neop,2017,22(4):235-249.
[32]	ARGOV-ARGAMAN N,MESILATI-STAHY R,MAGEN Y,et al.Elevated concentrate-to-forage ratio in dairy cow rations is associated with a shift in the diameter of milk fat globules and remodeling of their membranes[J].J Dairy Sci,2014,97(10):6286-6295.
[33]	张海波.日粮补充棕榈酸对泌乳早期荷斯坦奶牛泌乳性能、血液生化指标和激素水平的影响[J].畜牧兽医学报,2019,50(1):203-210.ZHANG H B.Effects of dietary supplementation of palmitic acid on lactation performance,blood biochemical indexes and hormone concentration in early lactation holstein dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2019,50(1):203-210. (in Chinese)
[34]	邢智洋,张梦璐,张菡,等.沉默SCAP基因对奶牛乳腺上皮细胞脂滴的影响[J].畜牧兽医学报,2019,50(3): 507-516.XING Z Y,ZHANG M L,ZHANG H,et al.Effect of silencing SCAP gene on lipid droplets in dairy mammary epithelial cells[J].Acta Veterinaria et Zootechnica Sinica,2019,50(3):507-516. (in Chinese)

Effect of Conjugated Linoleic Acid on the Size and Distribution of Milk Fat Globule in Dairy Cows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 34

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CAO Jianhua, YANG Baigao, ZHANG Peipei, FENG Xiaoyi, ZHANG Hang, YU Zhou, NIU Yifan, HAO Haisheng, DU Weihua, ZHU Huabin, YANG Ling, ZHAO Xueming. Mechanisms of Negative Energy Balance Affects Follicular Development in Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 22-30.
[2]	MENG Lu, HU Haiyan, DONG Lei, ZHENG Nan, WANG Jiaqi. Influence of Dairy Farm Environment on Mastitis Milk Microbiota via SourceTracker [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3872-3883.
[3]	ZHANG Hang, YANG Baigao, XU Xi, FENG Xiaoyi, DU Weihua, HAO Haisheng, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Research Progress on the Mechanism of Heat Stress Affecting the Development of Dairy Cow Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2692-2700.
[4]	ZHAO Wanli, CAO Qiqi, YANG Yue, DENG Zhaoju, XU Chuang. The Interaction between Gastrointestinal Microbiota and Mucosal Immunity in Health of Perinatal Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2751-2760.
[5]	HUANG Shangzhen, MA Longgang, LOU Wenqi, NING Jingyang, ZHANG Hailiang, HU Lirong, ZHA Qiong, LI Bin, XU Qing, BASANG Luobu, WANG Yachun. Analysis of Influencing Factors on Blood Indicators of Dairy Cows at High-altitude Area [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1964-1978.
[6]	FENG Xiaoyi, YANG Baigao, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Mechanism and Solution of Heat Stress Induced Embryo Quality Decline in Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 868-876.
[7]	YU Shiqiang, LI Liuxue, ZHAO Xiaobo, ZHAO Huiying, TU Yan, ZHAO Yuchao, JIANG Linshu. Differences and Correlations of Lactation Performance in Chinese Holstein Dairy Cows at Different Lactation Stages and Somatic Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1003-1014.
[8]	PAN Chanyuan, ZHAO Zixuan, DUAN Mingjie, JIANG Linshu, TONG Jinjin. The Mechanism of Artemisia carvifolia Alleviating Dairy Cow Oxidative Stress Predicted by Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1071-1084.
[9]	FAN Lei, SHEN Yu, YOU Liuchao, TIAN Xinyu, LUO Hao, WANG Xin, ZHANG Tingting, SHEN Liuhong. Research Progress on Abnormal Glucose and Lipid Metabolism in Dairy Cows Induced by Lipopolysaccharide (LPS) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 484-493.
[10]	LIU Aiyu, FENG Man, LI Yongliang, ZHAO Xinnian, GUO Jianjun, WANG Yanan, GAO Yuhong. Correlation Analysis between Environmental Parameters of Rolling Shed and Lactation Performance and Behavior of Dairy Cow [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 617-629.
[11]	DU Haidong, NA Renhua. Research Progress on Physiological Metabolism and Microbial Changes of Ruminants During Gestation and Lactation and Their Effects on Offspring Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4458-4467.
[12]	SHAN Qiang, WANG Xue, ZHU Yaohong, WANG Jiufeng. Application Prospect of Anti-inflammatory Mechanism of Lactobacillus rhamnosus and Its Prevention and Treatment in Livestock Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4537-4550.
[13]	SHI Rui, SU Guosheng, CHEN Ziwei, LI Xiang, LUO Hanpeng, LIU Lin, GUO Gang, ZHANG Yi, WANG Yachun, ZHANG Shengli, ZHANG Qin. Comparisons of Genomic Predictions for Fertility Traits in Chinese Holstein Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2944-2954.
[14]	ZHOU Fuzhen, ZHOU Bu, DAI Xu, WANG Haiyang, GUO Mengling, LIANG Yan, YANG Zhangping, MAO Yongjiang. Analysis on the Change of BCS in Early Lactation and Its Effects on Production Performance and Productive Lifespan in Holstein Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2955-2969.
[15]	HU Liping, SHEN Ziliang, WANG Quan, YU Zitong, ZHANG Qiqi, MAO Yongjiang, YANG Zhangping, ZHANG Huimin. Changes of Rumen Microbe and Milk Fatty Acid Composition during Early Lactation [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3018-3028.