纳米硒对氟化钠诱导小鼠肝组织细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2021.05.028

Abstract

Abstract: In order to explore the effect of nano-selenium on the apoptosis of hepatocytes induced by fluoride, A total of 40 Kunming mice were divided into 4 groups (each group contents 10 mices) after 7 days of adaptive feeding, which were administered with normal saline, 24 mg·kg^-1 NaF, 1 mg·kg^-1 nano-selenium and the same dose of NaF (24 mg·kg^-1 NaF) + nano-selenium (1 mg·kg^-1). The entire experiment lasted for 28 days, during which all the mice had free drinking and eating. The pathological damage of hepatocyte was evaluated by HE staining, while Tunel staining was used to evaluate the apoptosis level of hepatocytes, and the expression level of apoptosis-related genes and proteins were detected by immunofluorescence, Western blot, and RT-qPCR. The results showed that nano-selenium effectively alleviates the swelling of liver cells, vacuolar degeneration and nuclear fragmentation caused by fluoride treatment, and down-regulates the expression of genes related to apoptosis, such as Bax, Caspase3/9 and protein P53, Caspase3, elevated Bcl-2/Bax expression level. Tunel staining results showed that fluoride treatment significantly increased the rate of cell apoptosis (P<0.01), while nano-selenium can effectively reduce the incidence of cell apoptosis(P<0.05). The expression level of Cyt-C in the fluoride group was significantly higher than that in the control group (P<0.05), while the expression in the nano-selenium treatment group showed a downward trend. In conclusion, nano-selenium can effectively alleviate fluoride induced hepatocyte apoptosis by regulating the expression of apoptosis-related factors.

Key words: fluorosis, nano-Se, apoptosis, hepatotoxicity, cytokines

CLC Number:

S856.9

LIU Bingxian, ZENG Qiwen, CHEN Hanming, PANG Congying, LIU Yingwei, YANG Bijing, YI Jiangnan, LI Yuanliang, LI Yangwei, TANG Zhaoxin, LI Ying, ZHANG Hui. Effect of Nano-selenium on the Apoptosis Induced by Fluoride in Hepatocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1424-1431.

References 29

[1]	GUTH S, HÜSER S, ROTH A, et al. Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses[J]. Arch Toxicol, 2020, 94(5):1375-1415.
[2]	SRIVASTAVA S, FLORA S J S. Fluoride in drinking water and skeletal fluorosis:a review of the global impact[J]. Curr Envir Health Rpt, 2020, 7(2):140-146.
[3]	YANG S Y, ZHANG L, MIAO K K, et al. Effects of selenium intervention on chronic fluorosis-induced renal cell apoptosis in rats[J]. Biol Trace Elem Res, 2013, 153(1-3):237-242.
[4]	BUCCELLATO L J, TSO M, AKINCI O I, et al. Reactive oxygen species are required for hyperoxia-induced Bax activation and cell death in alveolar epithelial cells[J]. J Biol Chem, 2004, 279(8):6753-6760.
[5]	WANG Y X, XIAO X, ZHAN X A. Antagonistic effects of different selenium sources on growth inhibition, oxidative damage, and apoptosis induced by fluorine in broilers[J]. Poult Sci, 2018, 97(9):3207-3217.
[6]	TIAN X X, ZHANG H, ZHAO Y L, et al. Transcriptome analysis reveals the molecular mechanism of hepatic metabolism disorder caused by chromium poisoning in chickens[J]. Environ Sci Pollut Res, 2018, 25(16):15411-15421.
[7]	ANURADHA C D, KANNO S, HIRANO S. Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells[J]. Free Radic Biol Med, 2001, 31(3):367-373.
[8]	李素娟, 邢艳刚, 薛星晨, 等. 氟对小鼠睾丸谷胱甘肽抗氧化系统的影响[J]. 畜牧兽医学报, 2017, 48(5): 954-962.LI S J, XING Y G, XUE X C, et al. Effect of fluoride on glutathione antioxidant system in mice testis [J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(5): 954-962. (in Chinese)
[9]	GAO J P, WANG Y, XU G Q, et al. Selenium attenuates apoptosis and p-AMPK expressions in fluoride-induced NRK-52E cells[J]. Environ Sci Pollut Res, 2019, 26(15):15685-15697.
[10]	裴若男, 杨帆, 廖建昭, 等. 高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响[J]. 畜牧兽医学报, 2019, 50(9): 1912-1919.PEI R N, YANG F, LIAO J Z, et al. The effect of high dietary copper on oxidative damage and expression of Nrf2 signaling pathway related genes in the kidneys of broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(9): 1912-1919. (in Chinese)
[11]	ZHAO W P, WANG H W, LIU J, et al. JNK/STAT signalling pathway is involved in fluoride-induced follicular developmental dysplasia in female mice[J]. Chemosphere (Oxford), 2018, 209:88-95.
[12]	KHANDARE A L, GOURINENI S R, VALIDANDI V. Dental fluorosis, nutritional status, kidney damage, and thyroid function along with bone metabolic indicators in school-going children living in fluoride-affected hilly areas of Doda district, Jammu and Kashmir, India[J]. Environ Monit Assess, 2017, 189(11):579.
[13]	ZHAN X A, WANG M, XU Z R, et al. Evaluation of caspase-dependent apoptosis during fluoride-induced liver lesion in pigs[J]. Arch Toxicol, 2006, 80(2):74-80.
[14]	WANG H W, LIU J, WEI S S, et al. Mitochondrial respiratory chain damage and mitochondrial fusion disorder are involved in liver dysfunction of fluoride-induced mice[J]. Chemosphere (Oxford), 2020, 241:125099.
[15]	GUNEY M, ORAL B, TAKE G, et al. Effect of fluoride intoxication on endometrial apoptosis and lipid peroxidation in rats:role of vitamins E and C[J]. Toxicology, 2007, 231(2-3):215-223.
[16]	HAN B, YOON S, SU J L, et al. Effects of selenium, copper and magnesium on antioxidant enzymes and lipid peroxidation in bovine fluorosis[J]. Asian-Australas J Anim Sci, 2004, 17(12):1695-1699.
[17]	ZHOU B H, ZHAO J, LIU J, et al. Fluoride-induced oxidative stress is involved in the morphological damage and dysfunction of liver in female mice[J]. Chemosphere (Oxford), 2015, 139:504-511.
[18]	GAIGNARD P, LIERE P, THÉROND P, et al. Role of sex hormones on brain mitochondrial function, with special reference to aging and neurodegenerative diseases[J]. Front Aging Neurosci, 2017, 9:406.
[19]	CHERAGHI G, HAJIABEDI E, NIAGHI B, et al. High doses of sodium tungstate can promote mitochondrial dysfunction and oxidative stress in isolated mitochondria[J]. J Biochem Mol Toxicol, 2019, 33(4):e22266.
[20]	NI J, ZHONG Z, ZHANG W, et al. Fluoride resistance in fibroblasts is conferred via reduced susceptibility to oxidative stress and apoptosis[J]. FEBS Open Bio, 2020, 10(3):362-370.
[21]	宋霞, 陈涛, 张炜, 等. 高三尖杉酯碱对HL60细胞程序性凋亡的影响及机制研究[J]. 国际检验医学杂志, 2018, 39(20):2471-2474.SONG X, CHEN T, ZHANG W, et al. Mechanism and influences of Homoharringtonine on apoptosis of HL 60 human acute myeloid leukemia cell line[J]. International Journal of Laboratory Medicine, 2018, 39(20):2471-2474. (in Chinese)
[22]	郭英. 黄芪甲苷通过抑制AKT和NF-κB通路诱导胃癌MGC-803细胞凋亡[J]. 国际检验医学杂志, 2018, 39(19):2341-2344.GUO Y. Astragaloside induces gastric MGC-803 cells apoptosis by inhibiting AKT and NF-κB pathway[J]. International Journal of Laboratory Medicine, 2018, 39(19):2341-2344. (in Chinese)
[23]	王卫东, 陈正堂. Bcl-2/Bax比率与细胞“命运”[J]. 中国肿瘤生物治疗杂志, 2007, 14(4):393-396.WANG W D, CHEN Z T. Bcl-2/Bax ratio and the “life or death fate” of cells[J]. Chinese Journal of Cancer Biotherapy, 2007, 14(4):393-396. (in Chinese)
[24]	KANDA T, MATSUOKA S, YAMAZAKI M, et al. Apoptosis and non-alcoholic fatty liver diseases[J]. World J Gastroenterol, 2018, 24(25):2661-2672.
[25]	BAKER P R, FRIEDMAN J E. Mitochondrial role in the neonatal predisposition to developing nonalcoholic fatty liver disease[J]. J Clin Invest, 2018, 128(9):3692-3703.
[26]	MIAO K K, ZHANG L, YANG S Y, et al. Intervention of selenium on apoptosis and Fas/FasL expressions in the liver of fluoride-exposed rats[J]. Environ Toxicol Pharmacol, 2013, 36(3):913-920.
[27]	YOON I, WERNER T M, BUTLER J M. Effect of source and concentration of selenium on growth performance and selenium retention in broiler chickens[J]. Poult Sci, 2007, 86(4):727-730.
[28]	林长光，林金玉，刘东霞，等. 不同硒源对断奶仔猪生长性能、血清抗氧化能力和血浆硒含量的影响[J]. 畜牧兽医学报, 2013, 44(11): 1790-1796.LIN C G, LIN J Y, LIU D X, et al. Effect of different selenium sources on growth performance, plasma selenium content and anti-oxidative capacity in weaned piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(11): 1790-1796. (in Chinese)
[29]	FENG P, WEI J R, ZHANG Z G. Intervention of selenium on chronic fluorosis-induced injury of blood antioxidant capacity in rats[J]. Biol Trace Elem Res, 2011, 144(1-3):1024-1031.

Effect of Nano-selenium on the Apoptosis Induced by Fluoride in Hepatocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 29

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DONG Shucan, MAO Shuaixiang, WU Cuiying, LI Yaokun, SUN Baoli, GUO Yongqing, DENG Ming, LIU Dewu, LIU Guangbin. The Effect of the Androgen Receptor Inhibitor Enzalutamide on Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2022-2031.
[2]	WANG Jiying, YIN Ruiru, XIE Xing, WANG Haiyan, LIU Hudong, HU Hui, XIONG Qiyan, FENG Zhixin, SHAO Guoqing, YU Yanfei. Effects of LDH in Mesomycoplasma (Mycoplasma) hyopneumoniae on Apoptosis of Porcine Bronchial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2195-2205.
[3]	LI Qiuyun, TIAN Xinyuan, LIAO Wensheng, ZHANG Huanrong, REN Yupeng, YANG Falong, ZHU Jiangjiang, XIANG Hua. Effects of SOCS2 on Proliferation, Cycle and Apoptosis of Turbinate Bone Cells in Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2226-2240.
[4]	LUO Tongwang, WU Ya, WANG Shujie, SONG Houhui, SHAO Chunyan. Research Progress on the Mechanism of Cadmium Induced Liver Damage and Selenium Antagonizing Cadmium Hepatotoxicity [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1456-1466.
[5]	LAN Xinrui, ZHAO Baobao, ZHANG Bihan, LIN Xiaoyu, MA Huiming, WANG Yongsheng. Effects of β-sitosterol on Porcine Oocyte Maturation and Embryonic Development in Vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1629-1637.
[6]	HU Qiaoyan, ZHAI Xiangqin, LI Yidan, HAN Jiale, LEI Chuzhao, DANG Ruihua. Effects of bta-miR-101 on Proliferation, Apoptosis and Secretion of Bovine Testicular Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1040-1051.
[7]	LUO Chenghui, GAO Jiangrui, CHEN Junwei, WEI Chunjie, WEI Shuangshuang, PEI Yechun. Construction of Mouse Model of Dust Mite Induced Atopic Dermatitis and Asthma [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1257-1267.
[8]	HUO Yuannan, QIU Meijia, ZHANG Jiaojiao, YANG Weirong, WANG Xianzhong. Arginine and Its Metabolites Attenuate Heat Stress-induced Apoptosis of Immature Boar Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 587-597.
[9]	QIU Wenyue, SU Yiman, YE Jiali, ZHANG Xinting, PANG Xiaoyue, WANG Rongmei, XIE Zimao, ZHANG Hui, TANG Zhaoxin, SU Rongsheng. Study on Asiatic Acid Alleviates LPS-induced Acute Kidney Injury by Regulating Apoptosis and Autophagy of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 809-821.
[10]	CHEN Songbiao, LIU Feifei, SHANG Ke, YU Zuhua, HE Lei, WEI Ying, CHEN Jian, ZHANG Chunjie, CHENG Xiangchao, DING Ke. Molecular Mechanism of the “Battle” between Virus Infection and Host Antiviral Immunity-Apoptosis, Necroptosis and Pyroptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 59-70.
[11]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[12]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[13]	XU Xi, YANG Baigao, ZHANG Hang, FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3348-3357.
[14]	WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.
[15]	SHEN Tong, WANG Mengjie, WU Hua, LI Jinming, WANG Shuo, WU Xiaoqing, CHEN Xinlei, XING Qianwen, LIU Bo. Effect of Lycium ruthenicum Murray Anthocyanin on Hypoxia-induced Apoptosis in H9c2 Rat Cardiomyocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3490-3499.