2011-2015年环洞庭湖区H3亚型禽流感病毒的分离鉴定与遗传演化

doi:10.11843/j.issn.0366-6964.2019.02.016

Abstract

Abstract:

To better understand the situation and evolution of poultry infected with H3 subtype avian influenza viruses around Dongting Lake, active monitoring at live-poultry markets and Free-range duck farms at this region were implemented in 2011-2015, and a total of 31 strains of H3 subtype AIV were isolated. Gene sequencing and evolutionary analysis of these strains were conducted in our study. Sequencing results showed that all the viruses were located in the Eurasian lineage except for two H3N8s in North America lineage, all isolates were low pathogenic avian influenza virus. Phylogenetic tree showed that PA and PB2 genes of some viruses were clustered with H5, while NP, PA and PB1 were clustered with H7, suggesting that complicated gene exchange were occurred. Internal genes of some viruses isolated from free-range poultry show very high homology with wild birds origin H3, H7, H11 from neighboring countries such as Vietnam and South Korea, may share the same evolutionary origin. Further genome sequence alignment can divided all viruses into 21 different genotypes. The source of H3 subtype avian influenza virus in the Dongting Lake area is complicated, showing a clear genetic diversity.

CLC Number:

S852.659.5

HE Shicheng, PENG Zhi, WANG Weiguo, TANG Xiaoming, LU Xinghua, LIN Yuan, DENG Guohua, WANG Changjian. Isolation, Identification and Genetic Evolution of H3 Subtype Avian Influenza Virus in Dongting Lake Region from 2011 to 2015[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 382-389.

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References

[1] TAUBENBERGER J K, REID A H, KRAFFT A E, et al. Initial genetic characterization of the 1918"Spanish" influenza virus[J]. Science, 1997, 275(5307):1793-1796.
[2] NEUMANN G, NODA T, KAWAOKA Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus[J]. Nature, 2009, 459(7249):931-939.
[3] SCHOLTISSEK C, ROHDE W, VON HOYNINGEN V, et al. On the origin of the human influenza virus subtypes H2N2 and H3N2[J]. Virology, 1978, 87(1):13-20.
[4] PAPPAS C, VISWANATHAN K, CHANDRASEKARAN A, et al. Receptor specificity and transmission of H2N2 subtype viruses isolated from the pandemic of 1957[J]. PLoS One, 2010, 5(6):e11158.
[5] SONG M S, OH T K, MOON H J, et al. Ecology of H3 avian influenza viruses in Korea and assessment of their pathogenic potentials[J]. J Gen Virol, 2008, 89:949-957.
[6] ZHOU H B, ZHANG A D, CHEN H C, et al. Emergence of novel reassortant H3N2 influenza viruses among ducks in China[J]. Arch Virol, 2011, 156(6):1045-1048.
[7] SONG D, KANG B, LEE C, et al. Transmission of avian influenza virus (H3N2) to dogs[J]. Emerg Infect Dis, 2008, 14(5):741-746.
[8] ANTHONY S J, ST LEGER J A, PUGLIARES K, et al. Emergence of fatal avian influenza in New England harbor seals[J]. mBio, 2012, 3(4):e00166-12.
[9] LI C, YU M, LIU L T, et al. Characterization of a novel H3N2 influenza virus isolated from domestic ducks in China[J]. Virus Genes, 2016, 52(4):568-572.
[10] YANG D Q, LIU J, JU H B, et al. Genetic analysis of H3N2 avian influenza viruses isolated from live poultry markets and poultry slaughterhouses in Shanghai, China in 2013[J]. Virus Genes, 2015, 51(1):25-32.
[11] 崔鹏飞,彭志,张芳,等. 四株鸭源H3N8亚型禽流感病毒对SPF鸡的感染性分析[J]. 畜牧兽医学报, 2016, 47(6):1209-1214.
CUI P F, PENG Z, ZHANG F, et al. Infectivity of four H3N8 avian influenza viruses of duck origin in SPF chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(6):1209-1214. (in Chinese)
[12] 钟福生,王焰新,邓学建,等. 洞庭湖湿地珍稀濒危鸟类群落组成及多样性[J]. 生态环境, 2007, 16(5):1485-1491.
ZHONG F S, WANG Y X, DENG X J, et al. Species diversity of rare, endangered and national key protected waterfowls in Dongting Lake wetlands[J]. Ecology and Environment, 2007, 16(5):1485-1491. (in Chinese)
[13] MUNSTER V J, BAAS C, LEXMOND P, et al. Spatial, temporal, and species variation in prevalence of influenza A viruses in wild migratory birds[J]. PLoS Pathog, 2007, 3(5):e61.
[14] HOFFMANN E, STECH J, GUAN Y, et al. Universal primer set for the full-length amplification of all influenza A viruses[J]. Arch Virol, 2001, 146(12):2275-2289.
[15] KANG H M, LEE E K, SONG B M, et al. Novel reassortant influenza A(H5N8) viruses among inoculated domestic and wild ducks, South Korea, 2014[J]. Emerg Infect Dis, 2015, 21(2):298-304.
[16] POHLMANN A, STARICK E, GRUND C, et al. Swarm incursions of reassortants of highly pathogenic avian influenza virus strains H5N8 and H5N5, clade 2. 3. 4. 4b, Germany, winter 2016/17[J]. Sci Rep, 2018, 8(1):15.
[17] 彭志. 2014~2015年环洞庭湖与鄱阳湖散养鸭群禽流感病毒感染情况调查[D]. 长沙:湖南农业大学, 2016.
PENG Z. Investigation of infection of Avian Influenza in domestic duck in Dongting Lake and Poyang Lake from 2014 to 2015[D]. Changsha:Hunan Agricultural University, 2016. (in Chinese)
[18] 关立峥. 中国H3N2(2009-2014年)和H5(2015-2016年)亚型禽流感病毒的生物学特性研究[D]. 兰州:甘肃农业大学, 2017.
GUAN L Z. Biological characterization of H3N2(from 2009 to 2014) and H5(from 2015 to 2016) avian influenza viruses in China[D]. Lanzhou:Gansu Agricultural University, 2017. (in Chinese)

Isolation, Identification and Genetic Evolution of H3 Subtype Avian Influenza Virus in Dongting Lake Region from 2011 to 2015

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