2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

doi:10.11843/j.issn.0366-6964.2018.08.025

Abstract

Abstract:

Domestic poultry in live poultry markets (LPMs), especially waterfowl, play an important role in the evolution, maintenance and spread of the avian influenza viruses (AIVs). Therefore, it is important to conduct a long-term, regular epidemiological surveillance in the LPMs. From October 2016 to September 2017, we collected oropharyngeal and cloacal swabs from the poultry traded in the LPMs in the eastern China monthly. The samples were inoculated into SPF embryonated chicken eggs. Viruses in the allantoic fluid were confirmed by HA test and were divided to different subtypes by HI test with subtype-specific antibodies. Then eight segments of AIVs' genome were amplified by RT-PCR and these segments were sequenced. Sequence alignment and phylogenetic analysis were performed by software such as DNASTAR and MEGA6.0. A total of 1 178 samples from 127 flocks were collected, and 100 H9N2 AIV strains were isolated, among which 7 strains came from waterfowl. Genetic analysis of eight segments showed that all 7 strains belonged to the S genotype H9N2 AIVs. The genotype of H9N2 viruses from waterfowl traded in LPMs was consistent with that of predominant strains which were circulating in chickens in China. Our study highlights the need to constantly monitor the H9N2 viruses in poultry in LPMs to understand the emergence and evolution of strains that may become epidemic.

CLC Number:

S852.659.5

JIANG Da-xiu, MA Jing, WANG Xiao-quan, HU Shun-lin, LIU Xiao-wen, LIU Xiu-fan. Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1789-1796.

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Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017

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